A kind of ips cell preservation solution and preparation method thereof

A preservation solution and cell technology, applied in the field of IPS cell preservation solution and its preparation, can solve the problems of vascular toxicity, liver and kidney toxicity, protein denaturation, cytotoxicity, etc., and achieve the effects of clear composition, simple operation and high safety.

Active Publication Date: 2020-10-02
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cryopreservation effect of this invention is better, DMSO has cytotoxicity and interacts with protein hydrophobic groups, leading to protein denaturation, vascular toxicity and liver and kidney toxicity. It is toxic, and this traditional cryopreservation solution requires storage at ultra-low temperature and resuscitation before transplantation, which brings a lot of inconvenience to the application

Method used

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  • A kind of ips cell preservation solution and preparation method thereof
  • A kind of ips cell preservation solution and preparation method thereof
  • A kind of ips cell preservation solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 A kind of IPS cell preservation solution

[0026] The IPS cell preservation solution includes water and the following components per 100mL:

[0027] Albumin 0.1g, low molecular weight heparin sodium 0.1g, astragaloside IV 0.05g, glucose 0.1g, sodium chloride 0.5g, vitamin C 1.0g, glycine 0.2g, sodium acetate 0.4g, acetic acid 0.03g and glycerin 1.0g.

[0028] Preparation:

[0029] S1 Add sodium chloride and sodium acetate into 40mL water, stir and dissolve to obtain solution A;

[0030] S2 Add glycine to 20mL water, stir and dissolve to obtain solution B;

[0031] S3 Mix the solution A obtained in step S1 with the solution B obtained in step S2 evenly, add albumin, glucose, vitamin C, low molecular weight heparin sodium, astragaloside IV and glycerin while stirring, then add acetic acid, stir evenly, and dilute the water to 100mL, ready to serve.

Embodiment 2

[0032] Example 2 A kind of IPS cell preservation solution

[0033] The IPS cell preservation solution includes water and the following components per 100mL:

[0034] Albumin 0.3g, low molecular weight heparin sodium 0.2g, astragaloside IV 0.1g, glucose 0.2g, sodium chloride 1.0g, vitamin C 1.5g, glycine 0.4g, sodium acetate 0.5g, acetic acid 0.04g and glycerin 1.5g.

[0035] The preparation method is similar to Example 1.

Embodiment 3

[0036] Example 3 A kind of IPS cell preservation solution

[0037] The IPS cell preservation solution includes water and the following components per 100mL:

[0038] 0.5g of albumin, 0.3g of low molecular weight heparin sodium, 0.15g of astragaloside IV, 0.3g of glucose, 1.5g of sodium chloride, 2.0g of vitamin C, 0.6g of glycine, 0.6g of sodium acetate, 0.05g of acetic acid and 2.0g of glycerin.

[0039] The preparation method is similar to Example 1.

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Abstract

The invention belongs to the field of biomedicines and in particular relates to IPS (Induced Pluripotent Stem) cell preservation liquid and a preparation method thereof. The invention provides the IPS cell preservation liquid; each 100mL of the IPS cell preservation liquid is prepared from water and the following components: 0.1g to 0.5g of albumin, 0.1g to 0.3g of low molecular weight heparin, 0.05g to 0.15g of astragaloside, 0.1g to 0.3g of glucose, 0.5g to 1.5g of sodium chloride, 1.0g to 2.0g of vitamin C, 0.2g to 0.6g of glycine, 0.4g to 0.6g of sodium acetate, 0.03g to 0.05g of acetic acid and 1.0g to 2.0g of glycerol. After IPS cells are preserved by the IPS cell preservation liquid for 72h, the cell survival rate is more than 94 percent and the cell agglomeration rate is less than 0.4 percent; meanwhile, the IPS cell preservation liquid has definite components, is simple to operate and high in safety and has no toxic and side effects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an IPS cell preservation solution and a preparation method thereof. Background technique [0002] IPS cells (Induced Pluripotent Stem Cells) are manufactured by injecting some pluripotent genetic genes into skin and other cells, that is, to "initialize" ordinary body cells so that they have the function of stem cells. "IPS cells" are not only very similar to ES cells (embryonic stem cells) in terms of cell morphology, growth characteristics, and expression of stem cell markers, but also in terms of DNA methylation patterns, gene expression profiles, chromatin states, and formation of chimeric animals, etc. Also almost identical to ES cells. IPS cells can produce all cells except for embryos. If used in medical treatment, it can theoretically cure all diseases—all bad tissues are removed and replaced with regrown normal tissues. [0003] Chinese patent CN102365933B disclos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 车七石
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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