A method for separating and purifying β-cryptoxanthin

A technology for separating and purifying cryptoxanthin, applied in the field of molecular extraction, can solve the problems of restricting the development and application of beta-cryptoxanthin, unfavorable to industrialized production, complicated process, etc., and achieves good mechanical strength, convenient operation and good separation effect. Effect

Active Publication Date: 2019-01-11
云南宏绿辣素有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent 105073711A reports plant-derived β-cryptoxanthin and its preparation method. The process is complicated and cumbersome, which is not conducive to industrial production and greatly limits the development and application of β-cryptoxanthin.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for separating and purifying β-cryptoxanthin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for separating and purifying β-cryptoxanthin, the specific steps are as follows:

[0023] Take 120.3g of capsanthin, silica gel: 600g, 40-60 mesh, quality of capsanthin: silica gel mass=1:5, dissolve capsanthin with 600mL n-hexane, wet the sample on silica gel chromatography column, use n-hexane -Elute with acetone (40:1, V / V) to obtain 60.5g of crude β-cryptoxanthin; add 60.5g of 10% alcohol-alkali solution to the obtained crude β-cryptoxanthin, stir and mix, and perform saponification reaction under the protection of nitrogen and light. , The temperature is 55℃, and the time is 6h. After the saponification is completed, the alcohol solution is recovered and the saponification product is obtained; the saponification product is dissolved in 600ml ethyl acetate, and 300ml pure water is added for back extraction, standing for layering, recovering ethyl acetate, Obtained 49.5g of saponified β-cryptoxanthin ointment, with a β-cryptoxanthin content of 3.2%; put the sap...

Embodiment 2

[0025] A method for separating and purifying β-cryptoxanthin, the specific steps are as follows:

[0026] Take 1200g of capsanthin, silica gel: 6000g, 60-80 mesh, quality of capsanthin: silica gel quality=1:5, dissolve capsanthin with 6000mL n-hexane, wet the sample on silica gel chromatography column, use n-hexane- Acetone (40:1, V / V) was eluted to obtain 580.7g of crude β-cryptoxanthin; the obtained crude β-cryptoxanthin was added to 580.7g of 10% alcohol-alkali solution and stirred and mixed, and the saponification reaction was carried out under the protection of light and nitrogen. The temperature is 55°C and the time is 6h. After the saponification is completed, the alcohol solution is recovered and the saponification product is obtained; the saponification product is dissolved in 6000ml ethyl acetate, and 3000ml pure water is added for back extraction. The saponified β-cryptoxanthin ointment is 457.6g, and the β-cryptoxanthin content is 3.8%; the saponified β-cryptoxanthin ...

Embodiment 3

[0028] A method for separating and purifying β-cryptoxanthin, the specific steps are as follows:

[0029] Take 10kg of capsanthin, silica gel: 50kg, 200-300 mesh, quality of capsanthin: silica gel mass=1:5, dissolve capsanthin with 50L n-hexane, wet sample on silica gel chromatography column, use n-hexane- Acetone (40:1, V / V) was eluted to obtain 3.3kg of crude β-cryptoxanthin; the crude β-cryptoxanthin was added to 3.3kg of 12% alcohol-alkali solution, stirred and mixed, and the saponification reaction was carried out under the protection of nitrogen and protected from light. The temperature is 55℃, and the time is 6h. After the saponification is completed, the alcohol solution is recovered to obtain the saponification product; the saponification product is dissolved in 35L ethyl acetate, 15L pure water is added for back extraction, and the layers are allowed to stand for separation. After β-cryptoxanthin ointment is 2.8kg, its β-cryptoxanthin content is 3.2%; put the saponified...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for separating and purifying β-cryptoxanthin, using capsanthin as a raw material, and the specific steps are: using a silica gel chromatography column to conduct preliminary separation of β-cryptoxanthin in capsanthin; The separated β-cryptoxanthin is saponified; the saponified β-cryptoxanthin is initially concentrated by solvent extraction; the β-cryptoxanthin is further separated and purified by using a silica gel chromatography column again. The silica gel used in the present invention has high stability, good mechanical strength, convenient operation, good separation effect, solvent and silica gel can be recycled and reused, no three wastes are discharged, and is suitable for large-scale industrial production; through high-efficiency liquid phase detection, the silica gel prepared by the present invention β-Cryptoxanthin has high purity, its content can reach more than 75%, and it is a pure natural product.

Description

Technical field [0001] The invention relates to the technical field of molecular extraction, in particular to a method for separating and purifying β-cryptoxanthin. Background technique [0002] β-cryptoxanthin is a kind of natural carotenoid pigment, mainly found in higher plants, fungi, cyanobacteria, etc. It belongs to isoprene and its molecular formula is C 40 H 56 O, the molecular weight is 552.88. Its structural formula is as follows: [0003] [0004] β-cryptoxanthin is widely distributed in the human body and is detected in blood, lung, skin and oral mucosal cells. β-cryptoxanthin has good antioxidant properties and the ability to prevent chronic diseases. In terms of anti-cancer, β-cryptoxanthin has been regarded as a good anti-cancer factor, especially for lung cancer caused by smoking. Both β-carotene and β-cryptoxanthin have a synergistic effect in reducing the incidence of lung cancer. In addition, β-cryptoxanthin also has unique physiological functions in reducing...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 何志敏范明达韦勇
Owner 云南宏绿辣素有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap