Modified medical soybean protein bone adhesive, and preparation method thereof

A soybean protein and bone adhesive technology, which is applied in surgical adhesives, applications, medical science, etc., can solve the problems of low bonding strength of soybean protein adhesives and has not been widely used, and achieve enhanced bonding strength , promote bone tissue regeneration, and avoid painful effects

Active Publication Date: 2017-07-28
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low bond strength of soybean protein binders, it has not been widely used.

Method used

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  • Modified medical soybean protein bone adhesive, and preparation method thereof
  • Modified medical soybean protein bone adhesive, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Preparation of modified soybean protein isolate bone adhesive

[0014] At room temperature, dissolve soy protein isolate in 2 mL of phosphate buffer solution (pH=7.00, 0.05 mol / L), configure it into a 2% soy protein isolate solution, add 0.88g ascorbic acid at the same time, stir at 22℃ 10min, add 250~350μL tyrosinase solution (0.1mg / mL, 570U / mg), continue to stir the reaction at 22℃ for 6~12min, centrifuge, dialyze, freeze-dry to obtain modified soybean protein isolate.

[0015] The added amount of tyrosinase and the time for continuing the reaction as described in Table 1 below were respectively used to prepare soybean protein isolate A to I.

[0016] Table 1 Conditions of tyrosinase modification of soybean protein isolate and dopa yield

[0017]

[0018] Separately sample 20uL of the above soybean protein isolate A~I for analysis, repeat three times, add 20uL HCl (2mol / L), 20uL NaOH (2mol / L), 20uL solution (15% sodium molybdate and 15% nitrous acid) to each sample....

Embodiment 2

[0020] Example 2 Modified soy protein isolate bone adhesive and determination of its bonding strength

[0021] The modified soy protein isolate E obtained in Example 1 was dissolved in a phosphate buffer with pH=7.00, and the concentration shown in Table 2 was formulated into a 2-15% modified soy protein isolate adhesive, and then coated Place it on the bone fracture, fix it and soak it in anhydrous calcium chloride for 15 minutes, and fix it in an incubator at 25°C for 48 hours, then measure its adhesive strength on a universal testing machine and compare it with unmodified soy protein isolate , The test results are shown in Table 2 below.

[0022] Table 2 Bond strength of soy protein isolate bone adhesive

[0023]

[0024] It can be seen from Table 2 that the adhesive strength of the soy protein isolate bone adhesive modified by tyrosinase is enhanced, and the adhesive strength of the 5% modified soy protein isolate bone adhesive has the most obvious increase.

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Abstract

The invention relates to a modified medical soybean protein bone adhesive, and a preparation method thereof. The preparation method comprises following steps: soybean protein isolate is dissolved in a phosphate buffered solution, ascorbic acid is added, an obtained mixture is stirred for 10min of reaction at 22 DEG C, 250 to 350<mu>L of a tyrosinase solution is added, an obtained mixed material is stirred continuously for 6 to 12min of reaction, and at last, centrifugation, dialysis, and freeze drying are carried out so as to obtain modified soybean protein isolate; the obtained improved soybean protein isolate is dissolved in the phosphate buffered solution so as to prepare the 2 to 15% modified medical soybean protein bone adhesive. In applications, the modified medical soybean protein bone adhesive is used for coating broken bone for bonding. The modified medical soybean protein bone adhesive is high in adhesive strength and biocompatibility, and is capable of promoting bone regeneration.

Description

Technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a modified medical soybean protein isolate bone adhesive with high bonding strength and good biocompatibility and a preparation method thereof. Background technique [0002] Medical adhesive refers to an auxiliary agent that can polymerize in the body to quickly bond between tissues or between tissues and non-tissues. It is mainly used for local adhesion and repair of organs or tissues, auxiliary hemostasis for traditional sutures, and bone or joint Technical fields such as integration and positioning. The fibrin adhesives that have been marketed and used in clinical applications are mainly extracted and prepared. Fibrin adhesive has the advantages of biocompatibility, good plasticity, easy absorption by tissues, no cytotoxicity, and convenient preparation. But at the same time, there are disadvantages such as too small adhesive strength, which may cause virus infection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/001A61L24/0042A61L24/108C08L89/00
Inventor 沈新春莫家露冯晨俞凌孔令艳汤晓智周建新
Owner NANJING UNIV OF FINANCE & ECONOMICS
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