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Application of cell growth factor in treatment of ulcer diseases and pulmonary fibrosis diseases

A technology for hepatocyte growth factor and ulcer disease, which is applied in the application field of cell growth factor in the treatment of ulcer disease and pulmonary fibrosis disease, and can solve the problems of low efficiency, unseen and difficult application, etc.

Inactive Publication Date: 2017-08-15
北京三有利科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported (Long X, Xiong SD et al. Chin Med J, 2007, 120(16): 1432-1437.Effect of intramuscular injection of hepatocyte growth factor plasmamid DNA with electroporation on bleomycin-induced lung fibrosis in rats) application of gene recombination Plasmids have achieved certain effects in preventing and treating pulmonary fibrosis. Because it is injected intramuscularly by electric shock, it shows that the efficiency is still low, and after intramuscular injection, HGF secreted into the bloodstream can be distributed in various tissues, which may cause the risk of inducing tumors, which is difficult to implement in practice. application
In addition, there are also pulmonary fibrosis diseases caused by dust. Because of their different pathogenesis, there have been no reports on the application of gene therapy.

Method used

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  • Application of cell growth factor in treatment of ulcer diseases and pulmonary fibrosis diseases
  • Application of cell growth factor in treatment of ulcer diseases and pulmonary fibrosis diseases
  • Application of cell growth factor in treatment of ulcer diseases and pulmonary fibrosis diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0147] (1) Combination of recombinant plasmids pcKH and pUDKH carrying human hepatocyte growth factor gene and recombinant adenovirus Ad-HGF Construction and preparation

[0148] (A) Construction of pcKH plasmid

[0149] Using conventional molecular biology techniques, from a human placenta cDNA library (purchased from Clontech, the U.S.) by polymerase chain reaction (PCR) (upstream primer is 5'-ccatcgatgttaacatgtgggtgaccaaactc-3'[SEQ ID NO.01], downstream primer is 5 '-tgggatccgcggccgcctatgactgtggtacctt-3'[SEQ ID NO.02]) isolated the human HGF coding region cDNA (2184bp), sequenced and conducted a comprehensive analysis. The comparative analysis of the long cDNA sequence showed that the human HGF cDNA sequence we cloned was completely consistent with the human HGF full-length cDNA sequence published by Gene Bank. Then subclone it into the multiple cloning site (BamH I, Apa I site) of our self-reformed eukaryotic expression vector pcK to obtain the recombinant vector pc...

Embodiment 2

[0197] Embodiment 2: HGF recombinant plasmid pcKH and recombinant attenuated Salmonella SPH treat acute and chronic skin ulcers in rats Effect observation

[0198] (1) Effect of SPH on acute wound repair in Wistar rats

[0199] (1) Experimental animals and models:

[0200] Seven Wistar rats, male, weighing 220-240 grams, were provided by the Experimental Animal Center of the Academy of Military Medical Sciences. Purchased one week before the experiment, free to drink water and food, fasted on the day of the experiment, and the back hair was cleaned under anesthesia, with an area of ​​7×5cm. Make a mark at 1.5cm from each side of the spine, and use scissors to cut off the full-thickness skin along the mark to form two circular wounds, which are ready for use after hemostasis.

[0201] (2) SPH application method:

[0202] Seven Wistar rats were randomly divided into two groups and numbered. The left wounds of Nos. 1 to 7 belonged to the first group (normal saline grou...

Embodiment 3

[0266] Example 3: Observation of the Effect of Recombinant Attenuated Salmonella SPH on Rat Ulcerative Colitis

[0267] (1) Experimental animals and models:

[0268] 50 healthy Wistar rats, weighing 220-250 g, clean grade, were provided by the Animal Center of Gansu University of Traditional Chinese Medicine (SPF). 50 Wistar rats were fasted for 48 hours before the experiment, and randomly selected 40 rats on the 0th day to inhale ether for anesthesia. In the supine position, a polyethylene tube (about 2 mm in diameter) was inserted into the intestinal tract through the anus, with a depth of 8 cm, and 30 mg of (150mg / kg) equal volume solution (V / V) of TNBS and 50% ethanol was injected into the intestinal tract, and the anus was kept at a high position for 5 minutes to prevent the liquid from flowing out. same.

[0269] (2) Animal grouping and treatment:

[0270] Forty rats with TNBS enema were randomly divided into 4 groups: SP (attenuated Salmonella strain carrying e...

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Abstract

The invention relates to application of a cell growth factor in treatment of ulcer diseases and pulmonary fibrosis diseases, and in particular relates to application of a cell growth factor like a hepatocyte growth factor or a keratinocyte growth factor in preparation of a medicine used for preventing and / or treating the ulcer diseases and / or pulmonary fibrosis diseases. The invention also relates to a pharmaceutical composition containing the cell growth factor like the hepatocyte growth factor or the keratinocyte growth factor and a method for preventing and / or treating the ulcer diseases and / or pulmonary fibrosis diseases in a needed subject by using the hepatocyte growth factor or the keratinocyte growth factor. According to the invention, the ulcer diseases and pulmonary fibrosis diseases can be beneficially prevented and / or treated.

Description

[0001] This application is a divisional application of an invention application with the application number 201010000800.5, the application date being January 20, 2010, and the invention title being "Application of Cell Growth Factors for Treatment of Ulcer Disease and Pulmonary Fibrosis". technical field [0002] The present invention relates to the application of cell growth factor in the prevention and / or treatment of ulcer disease and pulmonary fibrosis, in particular to the application of hepatocyte growth factor in gene prevention and / or treatment of ulcer disease, especially chronic skin ulcer and pulmonary fibrosis It also relates to the application of keratinocyte growth factor in gene prevention and / or treatment of ulcer disease and pulmonary fibrosis. Background technique [0003] Acute skin wounds can generally heal by themselves, and measures such as application of drugs or growth factors can accelerate the healing. However, for chronic wounds caused by various ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K48/00A61P17/02A61P11/00A61P1/04
CPCA61K38/18A61K38/1833A61K48/005Y02A50/30
Inventor 哈小琴吴祖泽吕同德尹强王晓娜唐仲雄
Owner 北京三有利科技发展有限公司
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