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Device method of making artepillin c in propolis for Anti-cancer

A technology of propolis and artemisinin, which is applied in the field of preparing anticancer ingredient artemisinin C and anticancer ingredient artemisinin C in propolis, which can solve the problems of poison safety, doubts, and easy residue of organic solvents, etc.

Inactive Publication Date: 2017-08-15
ULTRA MICRORIGIN BIOMEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] And the existing technology of removing waxy or extracting flavonoids and artepillin C (artepillin C) components in propolis, such as European patent EP0976399B1, is to utilize ethanol or water to soak the pulverized propolis block to obtain propolis extract, and then anion exchange resin Treatment of flavonoids and artepillin C (artepillin C) components in the extract, this patent uses organic solvents to remove waxy components in propolis, which will cause serious losses of flavonoids and artepillin C (artepillin C) components, and organic solvents Easy to remain, causing toxicity and safety concerns
In addition, there are also supercritical CO 2 The method of extracting propolis by mixing fluid and solvent, this method belongs to a large degree of empirical work, requires a lot of time for testing, and after extraction, it needs to go through re-purification steps, and its practical value needs to be improved

Method used

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  • Device method of making artepillin c in propolis for Anti-cancer
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  • Device method of making artepillin c in propolis for Anti-cancer

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention and implement it, but the examples given are not intended to limit the present invention.

[0017] First, see figure 1 As shown, the method 100 for preparing the anticancer component artepillin C (artepillin C) in propolis according to a preferred embodiment of the present invention can prepare the anticancer effect artemisinin C (artepillin C) component, and the first step is to prepare Take the propolis ethanol extract 110: mix the propolis and ethanol to a saturated state and remove impurities, and then remove the impurities in the propolis ethanol mixture by centrifugation to obtain the propolis ethanol extract.

[0018] The second step of the present invention is separation 120: under the operating conditions of operating pressure 3000-4000psi, temperature 40-60°C, ...

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Abstract

A method of making artepillin C in propolis includes the following steps: A. Mix propolis with ethanol to obtain a propolis-ethanol extract; B. Provide supercritical carbon dioxide and the propolis-ethanol extract to a chromatographic column to separate wax and artepillin C, and then remove the wax at a bottom of the chromatographic column and collect the artepillin C containing isolate at a top of the chromatographic column; and C. Test the artepillin C by a cytotoxicity test, a cell morphology analysis, a cell cycle and apoptosis test, and a cell motility metastasis test to find an anti-cancer effect of the artepillin C containing isolate.

Description

technical field [0001] The present invention is related to the active ingredient of propolis, and more specifically refers to a method for preparing the anticancer ingredient artepillin C (artepillin C) in propolis and the anticancer ingredient artepillin C (artepillin C). Background technique [0002] Propolis has various functions such as anti-oxidation and anti-cancer. Many studies and papers have found or confirmed that propolis can effectively shrink tumors, kill cancer cells, inhibit the growth and metastasis of malignant tumors, reduce adverse reactions of chemotherapy, and improve anti-cancer immunity. This is because propolis contains flavonoids, artepillin C, enzymes and organic acids, which have the function of inhibiting and killing unhealthy cancer cells. [0003] In addition, there are existing propolis patents that disclose anti-cancer effects, such as Chinese patent CN 102885854 B, which shows that Taiwanese green propolis extracts can enhance the anti-cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/52C07C51/42C07C51/47A61P35/00
CPCC07C51/42C07C51/47C07C59/52A61P35/00G01N33/5011Y02P20/54
Inventor 庄明熙彭秋莹纪正祐锺心语刘曲婷郭蕙甄
Owner ULTRA MICRORIGIN BIOMEDICAL TECH
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