Method for determining viability of seeds of radix angelicae pubescentis

Abstract

The invention discloses a method for determining the viability of seeds of radix angelicae pubescentis. The method comprises the following steps: soaking the seeds of radix angelicae pubescentis into clean water for 8-12 hours, and filtering for later use; longitudinally cutting the seeds along middle lines on the backs of the seeds so as to respectively divide embryos and endosperms into two parts, putting one parts of embryos and endosperms into a TTC solution, and carrying out dyeing at a constant temperature of 25-45 DEG C for 50 minutes, wherein the TTC solution is prepared from a phosphate buffer solution, and the concentration of the TTC solution is 0.6%-1%; and after the dyeing is finished, flushing the seeds for 2-3 times with tap water, immediately observing coloring conditions of the embryos, and judging whether the seeds have viability. By virtue of the method, a rapid, simple and convenient method is provided for quality inspection of the viability of the seeds of radix angelicae pubescentis, and therefore, the rapid quality identification of the seeds of radix angelicae pubescentis in circulation and production is facilitated.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for measuring the viability of lovage seeds. Background technique [0002] Duhuo is the dried root of the Umbelliferae plant Angelica Placiformis. It is a commonly used Chinese medicinal material in my country. It has been artificially planted for more than 300 years. It is mainly produced in Hubei, Gansu and other provinces, with a planting area of ​​more than 100,000 mu. It has become a local rural area. Pillar industry. Most of the lovage seeds circulating in the market are produced by farmers scatteredly, and the quality levels are uneven. For this reason, we have used the four indicators of germination rate, thousand-grain weight, clarity, and water content to formulate quality grading standards for lovage seeds. At present, there are no lovage seeds. As for the determination technology of viability, there is only the determination method of germination rate of s...

AI Technical Summary

Problems solved by technology

However, the germination test of lovage seeds takes 1 month, which is a long period. At the same time, lovage seeds are not resistant to storage, and the quality of seeds decreases with the storage time.