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A kind of apple polyphenol oxidase activity promoter and its application

A technology of polyphenol oxidase and activity promoter, applied in the field of enzyme activity, can solve the problems of insufficient regulation of enzyme activity and high dosage, and achieve the effects of low critical micelle concentration, low dosage, and low Krafft point.

Active Publication Date: 2019-10-11
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the above data, it can be seen that the amount of traditional surfactants is relatively large, and the regulation effect on enzyme activity is not obvious enough.

Method used

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  • A kind of apple polyphenol oxidase activity promoter and its application
  • A kind of apple polyphenol oxidase activity promoter and its application
  • A kind of apple polyphenol oxidase activity promoter and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Preprocessing

[0054] The Shandong Yantai Red Fuji apples were washed in clean water, drained, and placed in a -20°C freezer for 12 hours.

[0055] 2. Extraction of crude enzyme solution

[0056] Flake the frozen apple, weigh 58.82 g, add 100 mL of phosphate buffer solution (0.2 M, pH=6.5, containing 0.25% (v / v) (250 μL) TritonX-100 and 2% (w / w) ) (3.38g) PVPP), the liquid-to-material ratio is 1.7mL / g, mix and beat for 1min, filter with four layers of ice gauze, separate the filtrate, centrifuge the obtained filtrate at 10000rpm for 10min at 4°C, take the supernatant, put it in Store in a -20°C freezer for later use.

[0057] 3. Determination of polyphenol oxidase activity

[0058] Using catechol as the substrate, the PPO crude extract was diluted 25 times and added to the accelerator to test the PPO enzyme activity. In the test system, the concentration of catechol was 150 mM. The accelerator is a compound of DTAB and 16-5-16, and its concentrations in the tes...

Embodiment 2

[0061] The accelerator is a compound of DTAB and 12-5-12, and its concentrations in the test system are: DTAB is 2.0 mM, and 12-5-12 is 20 μM. The rest are the same as in Example 1.

[0062] Compared with no accelerator, the enzyme activity of PPO was increased by 75% after adding accelerator.

Embodiment 3

[0064] The accelerator is a compound of CTAB and 16-5-16, and its concentrations in the test system are: CTAB is 0.3 mM, and 16-5-16 is 10 μM. The rest are the same as in Example 1.

[0065] Compared with no accelerator, the enzyme activity of PPO increased by 100% after adding accelerator.

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PUM

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Abstract

The invention relates to the technical field of enzyme activity, in particular to an apple polyphenol oxidase activity promoter and application thereof. The apple polyphenol oxidase activity promoter of the present invention comprises lauryltrimethylammonium bromide or hexadecyltrimethylammonium bromide and Gemini surfactant in a weight ratio of 10~2000:1~4 The mixture that mixes in proportion, described Gemini surfactant is dibromide-bis(dimethyldodecyl)pentammonium or dibromide-bis(dimethylhexadecyl)pentammonium. The present invention also includes the application of the above accelerator to improve the activity of apple polyphenol oxidase, which can increase the activity of apple polyphenol oxidase to 240%, and the consumption level of the accelerator is low.

Description

technical field [0001] The invention belongs to the technical field of enzyme activity, and in particular relates to an apple polyphenol oxidase activity promoter and application thereof. Background technique [0002] Polyphenol oxidase is a metalloprotease widely distributed in nature. It is ubiquitous in plants and can oxidize phenol or polyphenol to the corresponding quinone. It is widely used in food, medicine and environmental protection industries. Studying its activity can better solve the problems of food storage, plant resistance to pathogens and viruses, and biodegradation of phenolic organic matter-containing industrial wastewater. [0003] At present, most researchers study the purified polyphenol oxidase (PPO), including exploring the optimal activity conditions of the enzyme, the stimulation of the enzyme activity by surfactant (sodium dodecyl sulfate (SDS)), etc., in order to achieve the maximum possible to increase the enzymatic activity. PPO exists in a la...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02
Inventor 郭霞郭晶晶张礼飞万舒雨
Owner YANGZHOU UNIV
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