6 results about "Phenol oxidase activity" patented technology

The invention provides a sweet potato leaf powder processing technology with a high nutrition retention rate, which comprises the following steps: S1, putting sweet potato leaves into a dynamic fixation roller, and mixing nitrogen and carbon dioxide by adopting a nitrogen and carbon dioxide mixing generator to form nitrogen and carbon dioxide mixed gas, the nitrogen and carbon dioxide mixed gas is heated to 95 DEG C through a heat exchanger and sprayed out, the oxygen concentration in the dynamic fixation roller is maintained to be smaller than or equal to 0.5%, and the chlorophyll degradation rate of the sweet potato leaves is monitored through a near-infrared sensor by inhibiting the activity of polyphenol oxidase of the sweet potato leaves; according to the method, a weak acid environment is formed through CO2 dissolution to inhibit the activity of polyphenol oxidase and reduce the requirement for fixation temperature, active ingredients are reserved through three-area gradient variable-temperature drying and rapid dehydration and freeze-drying sublimation, energy consumption is reduced by 37%, heat stability is improved through polyphenol glycosylation, the bioavailability of beta-carotene is improved to 80% through nano embedding, and therefore nutrient loss is avoided, and the method is suitable for industrial production. The drying efficiency, color and flavor are improved.

The invention belongs to the technical field of intelligent food packaging films, and particularly relates to a preparation method and application of a biomass-based active packaging film. According to the film, a double-network skeleton is constructed by using wood nano cellulose / sodium alginate, so that the film is endowed with excellent flexibility, ultraviolet shielding property and antibacterial activity; furthermore, zein nanoparticles loaded with curcumin are uniformly embedded into the matrix, so that ammonia-sensitive color development and curcumin slow release synergism are realized. Due to the introduction of the curcumin-zein nanoparticles, the mechanical strength, the water-oxygen barrier property, the oxidation resistance and the antibacterial property of the film are further remarkably improved, and reversible color change occurs in the presence of NH3, so that the meat product decay visual detection is realized. Fresh shrimps are packaged with the film and stored at 4 DEG C, the activity of polyphenol oxidase can be remarkably inhibited, blackening and microbial proliferation are delayed, and a green, visible and controllable multifunctional packaging strategy is provided for a meat supply chain.

The invention discloses an efficient and universal tissue culture anti-browning method for sugarcane plants, and belongs to the technical field of tissue culture. According to the invention, the potassium cinnamate is used as an anti-browning active substance for the first time and is used in plant tissue culture pretreatment and induction culture treatment, and a synergistic strategy of low-temperature pretreatment, explant potassium cinnamate soaking and culture medium addition is introduced, so that a remarkable beneficial effect is achieved in sugarcane plant tissue culture anti-browning. Through synergistic application of low-temperature pretreatment and potassium cinnamate, the activity of polyphenol oxidase is inhibited in a targeted manner, and browning is effectively blocked. According to the method, the browning rate of different germplasm sugarcane plants is remarkably reduced, the regeneration efficiency is synchronously improved, the genotype dependence of a traditional browning resisting technology is successfully overcome, and an efficient and universal solution is provided for development and utilization of sugarcane germplasm resources.

The present application provides a wheat grain polyphenol oxidase activity major site and SNP marker and application, the SNP molecular marker Marker149631 is located on the 3A chromosome of wheat, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1 or SEQ ID NO.2; the SNP molecular marker Marker149773 is located on the 3A chromosome of wheat, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3 or SEQ ID NO.4; the SNP site provided by the present application is of great significance for detecting wheat grain low PPO activity variety, strain, breeding material, and molecular markers can be developed according to it, and the efficiency of wheat quality breeding is accelerated.

The invention discloses a nano antibody combined with polyphenol oxidase and application of the nano antibody. The nucleotide sequence of the nano antibody is as shown in SEQ ID NO.1, and the amino acid sequence corresponding to the nano antibody is as shown in SEQ ID NO.2. The nano antibody prepared by the invention has good stability, high specificity and high affinity, and can accurately inhibit the activity of polyphenol oxidase, thereby fundamentally preventing enzymatic browning; the prepared nano antibody is mainly used as a natural biological preservative and a processing aid, can be widely applied to quality maintenance of aquatic products and various easily browning foods, effectively blocks the enzymatic browning process by specifically inhibiting the activity of polyphenol oxidase, remarkably prolongs the shelf life and maintains the sensory quality of the product.

This invention provides the major active site of wheat grain polyphenol oxidase activity and the SNP marker Marker 149773, as well as their applications. The SNP molecular marker Marker 149631 is located on wheat chromosome 3A, and its nucleotide sequence is shown in SEQ ID NO. 1 or SEQ ID NO. 2. The SNP molecular marker Marker 149773 is located on wheat chromosome 3A, and its nucleotide sequence is shown in SEQ ID NO. 3 or SEQ ID NO. 4. The SNP sites provided by this invention are of great significance for detecting wheat varieties, lines, and breeding materials with low PPO activity. Molecular markers can be developed based on these sites to accelerate the efficiency of wheat quality breeding.