12 results about "Phenol oxidase activity" patented technology

The invention discloses a synergetic color protection method for hot-processed eggplants. The eggplants are inhibited from browning through combination of multiple technologies. The method comprises the following steps: pretreatment, preparation of a color protection solution (compounding of ascorbic acid, tea polyphenol and calcium chloride, ultrasonic high and low temperature circulating blanching, pulse vacuum treatment and gradient temperature control secondary enzyme deactivation. After being treated by the method disclosed by the invention, the polyphenoloxidase (PPO) activity can be synergistically inhibited, the cell permeability and the anti-oxidation effect can be regulated and controlled, and the color difference delta E value (less than 5.0) can be obviously reduced. The method is suitable for color protection pretreatment of eggplant processing forms such as hot drying, hot frying and hot steaming, the color protection efficiency is greatly improved, no synthetic preservative needs to be added, and a possible way is provided for deep development of eggplants in the field of prefabricated vegetables.

The invention provides a sweet potato leaf powder processing technology with a high nutrition retention rate, which comprises the following steps: S1, putting sweet potato leaves into a dynamic fixation roller, and mixing nitrogen and carbon dioxide by adopting a nitrogen and carbon dioxide mixing generator to form nitrogen and carbon dioxide mixed gas, the nitrogen and carbon dioxide mixed gas is heated to 95 DEG C through a heat exchanger and sprayed out, the oxygen concentration in the dynamic fixation roller is maintained to be smaller than or equal to 0.5%, and the chlorophyll degradation rate of the sweet potato leaves is monitored through a near-infrared sensor by inhibiting the activity of polyphenol oxidase of the sweet potato leaves; according to the method, a weak acid environment is formed through CO2 dissolution to inhibit the activity of polyphenol oxidase and reduce the requirement for fixation temperature, active ingredients are reserved through three-area gradient variable-temperature drying and rapid dehydration and freeze-drying sublimation, energy consumption is reduced by 37%, heat stability is improved through polyphenol glycosylation, the bioavailability of beta-carotene is improved to 80% through nano embedding, and therefore nutrient loss is avoided, and the method is suitable for industrial production. The drying efficiency, color and flavor are improved.

The invention belongs to the technical field of intelligent food packaging films, and particularly relates to a preparation method and application of a biomass-based active packaging film. According to the film, a double-network skeleton is constructed by using wood nano cellulose / sodium alginate, so that the film is endowed with excellent flexibility, ultraviolet shielding property and antibacterial activity; furthermore, zein nanoparticles loaded with curcumin are uniformly embedded into the matrix, so that ammonia-sensitive color development and curcumin slow release synergism are realized. Due to the introduction of the curcumin-zein nanoparticles, the mechanical strength, the water-oxygen barrier property, the oxidation resistance and the antibacterial property of the film are further remarkably improved, and reversible color change occurs in the presence of NH3, so that the meat product decay visual detection is realized. Fresh shrimps are packaged with the film and stored at 4 DEG C, the activity of polyphenol oxidase can be remarkably inhibited, blackening and microbial proliferation are delayed, and a green, visible and controllable multifunctional packaging strategy is provided for a meat supply chain.

The invention discloses an efficient and universal tissue culture anti-browning method for sugarcane plants, and belongs to the technical field of tissue culture. According to the invention, the potassium cinnamate is used as an anti-browning active substance for the first time and is used in plant tissue culture pretreatment and induction culture treatment, and a synergistic strategy of low-temperature pretreatment, explant potassium cinnamate soaking and culture medium addition is introduced, so that a remarkable beneficial effect is achieved in sugarcane plant tissue culture anti-browning. Through synergistic application of low-temperature pretreatment and potassium cinnamate, the activity of polyphenol oxidase is inhibited in a targeted manner, and browning is effectively blocked. According to the method, the browning rate of different germplasm sugarcane plants is remarkably reduced, the regeneration efficiency is synchronously improved, the genotype dependence of a traditional browning resisting technology is successfully overcome, and an efficient and universal solution is provided for development and utilization of sugarcane germplasm resources.

The invention provides a method capable of fermenting dendrocalamus latiflorus to produce natural sweet fragrance, and relates to the technical field of dendrocalamus latiflorus fermentation. The method capable of fermenting dendrocalamus latiflorus to produce natural sweet fragrance comprises the following specific steps: S1, firstly, peeling and cleaning dendrocalamus latiflorus raw materials, and after cleaning is completed, performing dicing treatment on dendrocalamus latiflorus; s2, soaking the dendrocalamus latiflorus raw materials subjected to dicing treatment in 0.1%-0.5% of vitamin C; and S3, heating water to 95 DEG C, keeping the constant temperature, then cleaning the dendrocalamus latiflorus raw materials soaked in the step S2 with water again, putting the dendrocalamus latiflorus raw materials into the heated water to be uniformly heated, and performing blanching enzyme deactivation. The dendrocalamus latiflorus is firstly soaked in a vitamin C solution before fermentation so as to temporarily inhibit the activity of polyphenol oxidase, then the polyphenol oxidase is inactivated by adopting a blanching enzyme deactivation mode so as to avoid browning and blackening, and then the dendrocalamus latiflorus is soaked in a calcium chloride solution so as to enhance the strength of cell walls, so that the survival rate of the dendrocalamus latiflorus is increased. And the dendrocalamus latiflorus is prevented from being soft and rotten.

The present application provides a wheat grain polyphenol oxidase activity major site and SNP marker and application, the SNP molecular marker Marker149631 is located on the 3A chromosome of wheat, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1 or SEQ ID NO.2; the SNP molecular marker Marker149773 is located on the 3A chromosome of wheat, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3 or SEQ ID NO.4; the SNP site provided by the present application is of great significance for detecting wheat grain low PPO activity variety, strain, breeding material, and molecular markers can be developed according to it, and the efficiency of wheat quality breeding is accelerated.

The invention discloses a nano antibody combined with polyphenol oxidase and application of the nano antibody. The nucleotide sequence of the nano antibody is as shown in SEQ ID NO.1, and the amino acid sequence corresponding to the nano antibody is as shown in SEQ ID NO.2. The nano antibody prepared by the invention has good stability, high specificity and high affinity, and can accurately inhibit the activity of polyphenol oxidase, thereby fundamentally preventing enzymatic browning; the prepared nano antibody is mainly used as a natural biological preservative and a processing aid, can be widely applied to quality maintenance of aquatic products and various easily browning foods, effectively blocks the enzymatic browning process by specifically inhibiting the activity of polyphenol oxidase, remarkably prolongs the shelf life and maintains the sensory quality of the product.

The present invention discloses the application of a cationic surfactant and mesoporous silica in synergistically inhibiting the activity of polyphenol oxidase. In the present invention, the cationic surfactant DTAB or [C 12 mim]Br is incubated with mesoporous silica nanoparticles together with polyphenol oxidase, which can cause more than 90% loss of the activity of polyphenol oxidase, and the usage amounts of the cationic surfactant DTAB and [C 12 mim]Br are significantly lower than the usage amounts when DTAB or [C 12 mim]Br is used alone.

This invention provides the major active site of wheat grain polyphenol oxidase activity and the SNP marker Marker 149773, as well as their applications. The SNP molecular marker Marker 149631 is located on wheat chromosome 3A, and its nucleotide sequence is shown in SEQ ID NO. 1 or SEQ ID NO. 2. The SNP molecular marker Marker 149773 is located on wheat chromosome 3A, and its nucleotide sequence is shown in SEQ ID NO. 3 or SEQ ID NO. 4. The SNP sites provided by this invention are of great significance for detecting wheat varieties, lines, and breeding materials with low PPO activity. Molecular markers can be developed based on these sites to accelerate the efficiency of wheat quality breeding.

The invention belongs to the technical field of wheat molecular breeding, and particularly relates to an SNP site associated with a wheat polyphenol oxidase active major gene. The SNP site has a polymorphic site of A / G allele mutation at the 36th basic group. In the application, the inventor tries to excavate and obtain key genetic loci and genes which can stably exist under multiple environment conditions and control the PPO activity of wheat on the basis of analyzing PPO activity characters of 207 representative wheat materials planted in different years by utilizing a whole genome association analysis method and combining a wheat 660K SNP chip; and the Whaas77577 molecular marker which is closely linked with the major gene qPPO3A.1 related to the PPO activity of the wheat is obtained. Based on the molecular marker, excellent allele variation type identification can be performed on breeding early generation materials, so that a scientific basis can be provided for wheat breeding progeny material selection.

To provide hexaploid wheat with low polyphenol oxidase activity and preserving quantitative trait locus Q.Ymym for wheat yellow mosaic disease resistance, and to provide a primer set that enables determination of whether or not a Triticum plant possesses a loss-of-function Ppo-D1 gene.SOLUTION: Provided is a hexaploid wheat having a homozygous type gene which is a Ppo-D1 gene with defective or reduced function of polyphenol oxidase activity and a quantitative trait locus Q.Ymym for resistance to wheat yellow mosaic disease, on chromosome 2D.SELECTED DRAWING: None

According to the invention, a cDNA sequence of the hunting orius venom serine protease inhibitor ScSPI5 is cloned, the cDNA sequence is as shown in SEQ ID No.1, and the coded amino acid is as shown in SEQ ID No.2. The recombinant protein obtained through insect baculovirus in-vitro expression can effectively inhibit the hemolymph phenol oxidase activity of agriculture and forestry pests such as tenebrio molitor, alunanensis and spodoptera frugiperda, and has the advantages that the activity of the hemolymph phenol oxidase can be effectively inhibited, the activity of the hemolymph phenol oxidase can be effectively inhibited, the activity of the hemolymph phenol oxidase can be effectively inhibited, the activity of the hemolymph phenol oxidase can be effectively inhibited, and the activity of the hemolymph phenol oxidase can be effectively inhibited. Therefore, the immunoreaction is inhibited, and the immunity is reduced. Meanwhile, the weight of larvae and pupae of spodoptera frugiperda can be reduced by orally taking the recombinant protein; tenebrio molitor pupae can be killed by injecting the recombinant protein, so that an important reference value is provided for developing a novel biological control insecticide.