Sweet strong high flavonoid cider and preparation method thereof
A technology for cider and high alcohol, applied in the preparation of alcoholic beverages, biochemical equipment and methods, methods based on microorganisms, etc.
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preparation example Construction
[0066] The preparation method of 0.5% hydrochloric acid methanol solution: mix 0.5 volume part of 35% concentrated hydrochloric acid with 99.5 volume parts methanol.
[0067] The pectinases used in the examples are all pectinases purchased from Ningxia Hersbit Biotechnology Co., Ltd. The relevant parameters are: solid powder, enzyme activity ≥ 500,000 u / g, and the recommended use condition is "pH3.2- 5.0, 10-50°C". Under the conditions of 50.0°C and pH 3.5, the amount of enzyme that catalyzes the hydrolysis of pectin to generate 1 μg of galacturonic acid in 1 minute is one pectinase activity unit (1u).
Embodiment 1
[0068] Example 1. Acquisition and Identification of Excellent Apple Germplasm 'CSR6R6-888'
[0069] The method for identifying whether an apple plant is R1R1 genotype, R6R6 genotype or R6R1 genotype is as follows: take the apple from the apple plant to be tested, extract the genomic DNA of the apple pulp, use the genomic DNA as a template, and use a primer pair composed of F3 and R3 PCR amplification, then judge the genotype according to the following standards: if the PCR amplification product is a band and is 497bp, the apple plant to be tested is R6R6 genotype; if the PCR amplification product is a band and is 386bp, the apple plant to be tested is R1R1 genotype; if the PCR amplification products are two bands and are 497bp and 386bp respectively, the apple plant to be tested is R6R1 genotype.
[0070] F3 (SEQ ID NO: 1): 5'-GGTGGTCAAAGATGTGTGTTGT-3';
[0071] R3 (SEQ ID NO: 2): 5'-TTTGCCTGCTACCCACTTCA-3'.
[0072] After testing, both 'Red Fuji' and 'Gala' apples were of R...
Embodiment 2
[0117] Embodiment 2, the acquisition of yeast strain
[0118] 1. Domestication and isolation of natural yeast
[0119] When the apples growing on the 'CSR6R6-888' plants are ripe, select good apples in the orchard and bring them back to the aseptic room, cut the peel into small pieces and put them into sterilized test tubes containing juice, and plug them with cotton plugs Place them in a constant temperature incubator at 25-28°C for 5-8 days. Yeast strains were isolated by streaking on plates, and three plates were made for each fermented mash, and cultured in an incubator at 28°C for 3 days. Observe the morphology of the colonies, pick well-separated and typical single colonies from the plate, and inoculate them on the slant of the test tube and the sterilized large test tube of apple juice, and select only 3 A single colony was numbered and marked to indicate the source of the strain. Place the slant of the test tube at 28°C and incubate for 3 days to check whether the b...
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