The invention discloses a preparation method of health-care
wine based on liquid-state fermented
cordyceps sinensis mycelia. The preparation method comprises the following steps: (1) activating strains and culturing a seed solution; (2) preparing a composite culture medium and performing enzymolysis pretreatment; (3) a staged synergistic
fermentation process; (4) efficiently breaking walls of mycelia and extracting
active components; and (5)
wine base compounding and stability optimization. Through the strategies of stepped carbon and
nitrogen source proportioning, composite enzymolysis and synergistic
fermentation, and dynamic regulation and control of dissolved
oxygen and material supplementation, the contents of
cordycepin (greater than or equal to 0.5 mg / g),
polysaccharide (greater than or equal to 20%) and
adenosine (greater than or equal to 0.15 mg / g) in mycelia are significantly increased; efficient wall breaking of
cell walls is realized by adopting an ultrasonic-low-temperature freeze-
drying combined technology, and the
dissolution rate of active ingredients is greater than or equal to 90%; and finally, compounding with a low-
alcohol-content
wine base, and supplementing with a natural
antioxidant to prepare a product with
flavor and health-care functions. The technical problems that traditional
cordyceps sinensis wine is low in
active ingredient content and poor in stability are solved, and the
cordyceps sinensis wine is suitable for large-scale production.