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Chicken infectious bursal disease inactivated vaccine and preparation method thereof

A chicken infectious bursal disease technology, applied in the field of chicken infectious bursal disease inactivated vaccine and its preparation, to achieve high immune level, stable efficacy and high protection rate

Active Publication Date: 2020-06-23
广州渔跃生物技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Schizophyllum polysaccharide (SPG) is a water-soluble polysaccharide extracted from the fruiting body, mycelium and fermentation broth of Schizophyllum fungus. Its structure is β-(1-3) glycoside as the main chain, with β-(1-6 ) β-D-glucan on the glycosidic side chain; canna flower is the flower of canna, a perennial herbaceous plant in the family Cannaceae; indomethacin is an anti-inflammatory drug for nonzizia. enhanced reporting

Method used

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  • Chicken infectious bursal disease inactivated vaccine and preparation method thereof
  • Chicken infectious bursal disease inactivated vaccine and preparation method thereof
  • Chicken infectious bursal disease inactivated vaccine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, the preparation of fresh canna flower extract

[0030] Take canna flowers or flower buds, take their petals, grind them, add distilled water, put them in a triangular flask, seal them, take a constant temperature water bath at 95°C for 30 minutes, cool them down rapidly, centrifuge them at room temperature for 10 minutes, and take the supernatant to obtain the final product.

[0031] Embodiment 2~4 of the present invention and comparative example 1~3 immunopotentiator formula and dosage

[0032]

Embodiment 5

[0033] Embodiment 5 The preparation of chicken infectious bursal disease inactivated vaccine of the present invention

[0034] Include the following steps:

[0035] A) Passage and culture of LMH cells: take LMH passage cell line and digest and passage with EDTA-trypsin cell dispersion solution, continue to culture in the culture flask with cell growth solution, when a good monolayer is formed, it is used for passaging or virus inoculation;

[0036] B) Virus inoculation: take chicken infectious bursal virus B87 strain, inoculate it into the prepared LMH passaged cells according to the final volume of 50:500, absorb the virus liquid at 37°C for 45 minutes, add maintenance solution, and place At 37°C, with 5% CO 2 Harvest the cell fluid when the cell lesion rate reaches more than 75%;

[0037] C) Propagation of seedling venom: take the cell line culture bottle that has formed a good monolayer in step A), discard the cell growth solution, inoculate the maintenance solution con...

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Abstract

The invention belongs to the field of biological products and in particular relates to an infectious bursal disease inactivated vaccine and a preparation method thereof. The method comprises the following steps: A) performing passage and culture on LMH cells; B) performing virus inoculation; C) propagating vaccine virus solution; and D) preparing the finished product. The vaccine prepared by the method is stable in potency, high in immunizing potency and high in safety, and the production process is simple and easy to operate.

Description

technical field [0001] The invention belongs to the field of biological products, and in particular relates to an inactivated vaccine for chicken infectious bursal disease and a preparation method thereof. Background technique [0002] Chicken infectious bursal disease (Infectious bursal disease IBD) is caused by infectious bursal disease virus (IBDV), an acute, highly contagious infectious disease that harms young chickens. Chicken infectious bursal disease is one of the important infectious diseases that seriously threaten the chicken industry in my country. On the one hand, it has a high mortality rate and a high elimination rate; The level drops, resulting in immune failure, which increases the susceptibility of chickens to pathogens and causes huge economic losses. [0003] At present, domestic vaccines used to prevent and treat IBD are mainly divided into live vaccines and inactivated vaccines. Live vaccines are highly virulent, but they are likely to cause serious da...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61P31/14A61K39/39
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/552A61K2039/55511A61K2039/55583A61K2039/55588C12N2720/10034A61K2300/00
Inventor 张毓金谢秉超严悌昆
Owner 广州渔跃生物技术有限公司
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