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An immobilized bacterial agent for oil pollution remediation prepared by using kelp slag

A technology of kelp slag and offshore oil, applied in the directions of fixing on or in inorganic carriers, fixing on/in organic carriers, seawater treatment, etc. Environmental conditions and other issues to achieve the effect of accelerating biodegradation

Active Publication Date: 2020-12-04
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a bioremediation immobilized bacterial agent for marine oil spill polluted sediments, which effectively solves the problem that the liquid bacterial agent applied in bioremediation is easily affected by environmental conditions such as ocean currents, temperature, and nutrient salts

Method used

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  • An immobilized bacterial agent for oil pollution remediation prepared by using kelp slag
  • An immobilized bacterial agent for oil pollution remediation prepared by using kelp slag
  • An immobilized bacterial agent for oil pollution remediation prepared by using kelp slag

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1 Screening of immobilized materials and optimization of preparation methods

[0025] Screen the density, solidity, adsorption rate and dissolution loss rate indicators of the immobilized bacterial agent, and finally determine the best immobilized granule components and preparation methods.

[0026] The instruments used are as follows: constant temperature culture shaker: THZ-100 type, Shanghai Yiheng Scientific Instrument Company; electric heating constant temperature blast drying oven: Shanghai Yiheng Scientific Instrument Company; electronic balance: BSA224S-CW, Sartorius Company; large microbial fermentation Tank: 30L, 120L, Shanghai Guoqiang; 120A two-phase 3kw granulator: Linghang Agricultural Machinery Company; gas chromatography mass spectrometer (GC / MS): 6890N-5973N type, Agilent Company;

[0027] Kelp slag is the largest proportion of solid waste produced in the kelp industry, especially in the production of sodium alginate. When using, the kelp slag ...

Embodiment 2

[0078] To fix oil-degrading bacteria with the optimal raw material ratio, first mix 700g of kelp residue and 700ml of bacterial liquid, and bake them at 30-45°C until dry; then add 300g of kaolin and stir evenly; mix 5-15g of seaweed Dissolve sodium bicarbonate in 500-700ml of water, mix with the above raw materials, stir evenly, slowly put into the granulator, collect the obtained granules, and dry them in an oven at 30-45°C to obtain immobilized bacterial agents. The finished product is as follows: Figure 5 As shown, the bacterial agent is a white-brown cylindrical particle with a diameter of 1 cm and a height of 2 to 3 cm.

[0079] Add the liquid bacterial agent and immobilized bacterial agent containing the same amount of petroleum-degrading bacteria to the petroleum medium, and after 21 days of degradation experiments, determine the oil content of the immobilized bacterial agent, liquid bacterial agent and natural weathering (negative control) by gravimetric method. Degr...

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Abstract

The invention aims to provide an immobilized microbial inoculum applicable to bioremediation of marine spilled oil-polluted sediments. A used immobilizing material comprises kelp residues, kaolin and an adhesive. The kelp residue material provided by the invention can adsorb petroleum hydrocarbon and can be used as the immobilizing material for embedding petroleum-degrading bacteria, so that on one hand, the kelp residues actively adsorb the petroleum hydrocarbon which is used by the petroleum-degrading bacteria as a carbon source, and on the other hand, the immobilized petroleum-degrading bacteria are protected in a kelp residue matrix, can fixedly grow in a polluted area for a long time and can accelerate biodegradation of petroleum pollution.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of the environment polluted by petroleum and petroleum products, and in particular relates to an immobilized bacterial agent for petroleum pollution remediation prepared by using kelp slag. Background technique [0002] In recent years, with the exploitation of offshore oil resources and the transportation of offshore oil products, marine oil spill accidents have occurred frequently, causing serious ecological environment damage and economic losses. Therefore, finding a friendly and low-cost oil spill removal strategy is an urgent problem to be solved in the current marine environmental governance. Currently, many different approaches are used to mitigate and clean up oil spill pollution. Among them, the traditional physical and chemical methods are mainly aimed at the rapid disposal of sea oil spills, but in practical applications, the cleaning is often incomplete or causes the transforma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/02C12N11/14C12N11/10C02F3/34C02F103/08
CPCC02F3/343C02F2103/08C12N11/02C12N11/10C12N11/14
Inventor 郑立张毅然高伟李倩韩彬崔志松何昌飞高详兴栾晓
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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