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In Vitro Rapid Propagation of Yulu Using Leaves as Explants

A technology of explants and leaves, applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems that hinder the large-scale application of tissue culture in vitro rapid propagation, the low induction and differentiation rate of callus clump buds, and the inconvenience. With market-oriented production conditions and other problems, it can achieve the effects of low cost, good growth status, and short regeneration cycle.

Active Publication Date: 2021-02-09
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are successful precedents in the Yulu tissue culture experiment done by the predecessors, the induction rate of callus clustered buds is low, resulting in low output efficiency, which greatly hinders the large-scale in vitro rapid propagation of Yulu tissue culture in actual production. application, does not have market production conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012]N1 Yulu leaves were selected as explants for rapid propagation in vitro.

[0013]1. Explant inoculation and callus induction: Take the young leaves of the N1 Yulu plant, put them in a sterile ultra-clean table, soak them in 75% (volume / volume) alcohol for 60s, and rinse them with sterile water for 2 to 3 times Then, quickly pour it into 3% (mass / volume) sodium hypochlorite solution and soak for 8 minutes, pour off the sodium hypochlorite solution, and rinse with sterile water for 4 to 5 times. Cut the treated Yulu leaves into approximately 1 cm sections; Containing 6-BA 0.5mg / L, NAA 0.1mg / L, KT 1mg / L, sucrose 3.0% (mass / volume), agar 6.0% (mass / volume), on MS solid medium with pH 5.8, light The intensity is 2000 Lux, the light time is 12 hours a day, the culture temperature is 25°C, and the subculture is performed once every 50 days. Differentiation can be induced when the yellow-green granular callus grows.

[0014]2. Induction of clump buds: transfer the callus to the differentiat...

Embodiment 2

[0017]Select OB1 Yulu leaves as explants for rapid propagation in vitro.

[0018]1. Explant inoculation and callus induction: Take the young leaves of OB1 Yulu plant, put them in a sterile ultra-clean table, soak in 75% (volume / volume) alcohol for 45s, and rinse with sterile water for 2 to 3 times Then, quickly pour it into 1.5% (mass / volume) sodium hypochlorite solution and soak for 10 minutes, pour off the sodium hypochlorite solution, and rinse with sterile water for 4 to 5 times. Cut the treated Yulu leaves into approximately 1 cm sections; Containing 6-BA 0.5mg / L, NAA 0.1mg / L, KT 1mg / L, sucrose 3.0% (mass / volume), agar 6.0% (mass / volume), on MS solid medium with pH 6.0, light The intensity is 1500 Lux, the light time is 14 hours a day, the culture temperature is 24 ℃, and the subculture is performed once every 50 days. Differentiation can be induced when the yellow-green granular callus grows.

[0019]2. Induction of clump buds: transfer the callus to the differentiation medium: MS+K...

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PUM

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Abstract

The purpose of the present invention is to overcome the shortcomings such as slow propagation speed, unstable character, and low survival rate in the traditional Yulu propagation method; Factory production and other shortcomings. The invention provides a rapid in vitro propagation method of Yulu, which enables Yulu to produce a large number of regenerated plants with stable genetic background and consistent plant type in a relatively short period of time. Without affecting the normal development and reproduction of the mother plant, Yulu can be reproduced on a large scale in a relatively short period of time, and the material is not limited by seasons, the regeneration cycle is short, the callus induction rate is high, and the cluster bud differentiation rate is high. The growth state is good after transplanting. It is very suitable for rapid propagation of rare varieties of Yulu, and the average cost of regenerated seedlings per plant is extremely low, which is very suitable for factory production.

Description

Technical field[0001]The invention belongs to a method for flower propagation, and specifically relates to a method for asexual and rapid propagation of flowers.Background technique[0002]Yulu is a perennial herb of the Liliaceae family, a genus of twelve jujubes, with fleshy leaves and plump and plump leaves. Native to South Africa, it is resistant to drought and cold, avoiding high temperature, humidity and sun exposure. The young plants of Yulu are mostly solitary, and gradually become groups, with fleshy leaves arranged in compact rosettes. Yulu is exquisite and small in appearance, rich in variety, crystal clear leaves, and full of changes. It is deeply loved by flower lovers at home and abroad, and has very high market demand and economic value. Yulu's traditional propagation methods mostly use sowing propagation, cutting propagation and ramification propagation. Among them, cutting propagation and ramet propagation are asexual propagation, which can keep the plant's traits sta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 崔金腾袁成飞张克中
Owner BEIJING UNIV OF AGRI