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Method for in-vitro rapid propagation of Haworthia cooperi with leaf as explant

A technology of explants and leaves, applied in botany equipment and methods, horticultural methods, plant regeneration, etc. It has market-oriented production conditions and other issues, and achieves the effects of low cost, good growth state, and short regeneration cycle

Active Publication Date: 2017-09-15
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are successful precedents in the Yulu tissue culture experiment done by the predecessors, the induction rate of callus clustered buds is low, resulting in low output efficiency, which greatly hinders the large-scale in vitro rapid propagation of Yulu tissue culture in actual production. application, does not have market production conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] N1 Yulu leaves were selected as explants for in vitro rapid propagation.

[0013] 1. Explant inoculation and callus induction: Take the young leaves of N1 Yulu plant, put them in a sterile ultra-clean bench, soak them in 75% (volume / volume) alcohol for 60 seconds, and rinse them with sterile water 2-3 times After that, quickly pour it into 3% (mass / volume) sodium hypochlorite solution and soak for 8 minutes, pour off the sodium hypochlorite solution, and then rinse it with sterile water for 4 to 5 times, and cut the treated Yulu leaves into small sections of about 1 cm; Containing 6-BA 0.5mg / L, NAA 0.1mg / L, KT 1mg / L, sucrose 3.0% (mass / volume), agar 6.0% (mass / volume), pH value 5.8 on MS solid medium, light The intensity is 2000 Lux, the light time is 12 hours a day, the culture temperature is 25°C, and the culture is subcultured once every 50 days. The differentiation can be induced when yellow-green granular callus grows.

[0014] 2. Induction of clustered buds: the ...

Embodiment 2

[0017] The leaves of OB1 Yulu were selected as explants for in vitro rapid propagation.

[0018] 1. Explant inoculation and callus induction: Take the young leaves of OB1 Yulu plant, put them in a sterile ultra-clean bench, soak them in 75% (volume / volume) alcohol for 45 seconds, and rinse them with sterile water for 2-3 times After that, quickly pour it into 1.5% (mass / volume) sodium hypochlorite solution and soak for 10 minutes, pour off the sodium hypochlorite solution, and then rinse with sterile water for 4 to 5 times, and cut the treated Yulu leaves into 1cm sections; Containing 6-BA 0.5mg / L, NAA 0.1mg / L, KT 1mg / L, sucrose 3.0% (mass / volume), agar 6.0% (mass / volume), pH 6.0 MS solid medium, light The intensity is 1500 Lux, the light time is 14 hours per day, the culture temperature is 24°C, and the culture is subcultured once every 50 days. The differentiation can be induced when yellow-green granular callus grows.

[0019] 2. Induction of clustered buds: transfer callu...

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PUM

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Abstract

The objective of the invention is to overcome the disadvantages of slow propagation speed, instable properties, low survival rate and the like of traditional Haworthia cooperi propagation methods and the disadvantages of low induced differentiation rate of cluster buds, long regeneration period, unsuitability for industrial production and the like of conventional tissue culture tests of Haworthia cooperi. The invention provides a method for in-vitro rapid propagation of Haworthia cooperi. With the method, a great number of regenerated plantlets with stable genetic background and consistent plant types can be generated from Haworthia cooperi in a short period of time; large-scale propagation of Haworthia cooperi in a short period of time can be realized without influence to normal growth and propagation of mother plants; raw mother plants can be obtained without seasonal restriction; the period of regeneration is short; the inductivity of calluses is high; the differentiation rate of cluster buds is high; and the growth states of transplanted plantlets are good. The method provided by the invention is especially suitable for rapid propagation of rare varieties of Haworthia cooperi; average cost for each regenerated plantlet is low; and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to flower propagation method, in particular to the asexual rapid propagation method of flower. Background technique [0002] Yulu is a perennial herb belonging to the family Liliaceae, belonging to the genus Twelve, with fleshy, plump leaves. Native to South Africa, it is resistant to drought and cold, so avoid high temperature, humidity and sun exposure. Yulu young plants are mostly solitary, and then gradually grow in groups, and the fleshy leaves are arranged in compact rosettes. Jade dew is exquisite in shape, rich in variety, crystal clear and varied in leaf color. It is deeply loved by flower lovers at home and abroad, and has very high market demand and economic value. The traditional propagation method of Yulu mostly adopts sowing propagation, cutting propagation and division propagation. Among them, cutting propagation and division propagation are asexual reproduction, which can keep the traits of the plant stable, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 崔金腾袁成飞张克中
Owner BEIJING UNIV OF AGRI