A method for capturing acutely isolated nerve cells under a microscope
An acute separation and nerve cell technology, applied in the analysis of materials, individual particle analysis, particle and sedimentation analysis, etc., can solve problems such as rough surface, cells that do not meet the sealing conditions, and patch clamping that cannot be successfully completed
Inactive Publication Date: 2019-06-07
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology
However, the patch clamp system itself has high requirements on the cells, especially when studying acutely isolated nerve cells. The surface of the isolated nerve cells is not smooth, excessive digestion, etc. will cause these cells to fail to meet the sealing conditions, and thus cannot be successfully completed. patch clamp experiment
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[0063] The technical solution of the present invention will be described clearly and completely in conjunction with the accompanying drawings in the following.
[0064] figure 1 Shown is a schematic structural diagram of one of the embodiments of the acutely separated nerve cell capture system under the microscope of the present invention. The system includes a microscope electron eyepiece 1, a computer 2, a single-chip computer 3, a driver 4, a stepping motor, and a patch clamp system microscope carrier Table 7 in which the stepper motor includes a horizontal stepper motor 5 and a longitudinal stepper motor 6.
[0065] The patch clamp system microscope stage 7 is located under the microscope electron eyepiece 1, and is used to carry acutely separated nerve cells to be studied;
[0066] The electron eyepiece 1 of the microscope is connected with a computer 2 for obtaining cell images under the microscope and transmitting the obtained cell images to the computer 2. The electronic eye...
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The invention provides a microscopically acute separation nerve cell capturing system and a method, and relates to the field of patch clamp sealing cell capturing; the invention is especially applicable to rapidly capture acute separated nerve cell in line with sealing condition under the microscope in a patch clamp experiment. The system comprises a microscope electronic eyepiece, a computer, a single chip, a driver, a stepping motor and a patch clamp system microscope stage. The system provided by the invention can accurately capture the nerve cell according with the sealing condition, reject the cell which does not meet the sealing condition, and automatically move the microscope stage of the patch clamp system; thus the cell is moved to the middle of the microscope vision, time and spirit for looking for cells meeting the sealing condition in the patch clamp experiment can be greatly saved; moreover, the acute separation nerve cell capturing system can avoid dizziness, nausea and other bad reactions of experimenters working under the microscope for a long time; the system is mounted in the patch clamp system without influence on any other operation; therefore, the microscopically acute separation nerve cell capturing system is easy to popularize.
Description
Technical field [0001] The invention relates to the field of patch clamp sealing cell capture, in particular to a system and method for capturing acutely separated nerve cells under a microscope. Background technique [0002] The patch clamp technique is to use a special glass micropipette to be adsorbed on the cell surface to form a seal of 10 to 100 gigohms, and then voltage clamp or current clamp is performed on the patch to measure the ion channel opening. Changes in membrane current or membrane potential. Through the patch clamp system to observe the membrane current or membrane potential changes caused by the opening and closing of the channel, the relationship between them and the voltage, current, drug, etc. that induce the opening or closing of the channel can be analyzed. The patch clamp system has been widely used in physiology, pharmacology and other research work, and many universities are equipped with this equipment. However, the patch clamp system itself has hig...
Claims
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IPC IPC(8): G01N15/10
CPCG01N15/1031G01N2015/1006
Inventor 陈素成波胡星
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES