A three-dimensional electrofocusing microfluidic chip for solid-phase microextraction and its manufacturing method

Abstract

The invention discloses a three-dimensional electrofocusing micro-fluidic chip for solid-phase micro extraction and a manufacturing method of the three-dimensional electrofocusing micro-fluidic chip. The chip comprises a gas-path liquid-path layer and a gas-path layer, or two identical gas-path liquid-path layers, wherein the gas-path liquid-path layer comprises a gas-path channel, a gas-path storage tank, a liquid-path channel and a liquid-path storage tank; the liquid-path channel defines a bottle neck structure for storing solid-phase extract granules; the gas-path layer is of a structure identical to that of a gas path in the gas-path liquid-path layer and comprises the gas-path channel and the gas-path storage tank connected with the gas-path channel; in the gas-path liquid-path layer, the gas-path channel is a channel from the gas-path storage tank, the channel is divided into two gas-path channels from the middle, and the two gas-path channels are symmetrically distributed on two sides of the liquid-path channel and are crossed and communicated at the liquid-path channel to form a nozzle finally; the depth of the gas-path channel is greater than that of the liquid-path channel; the outlet of the liquid-path channel is positioned in the middle of the gas-path channel in both the transverse direction and the depth direction of the channel. By adopting the chip, dual functions of solid-phase micro extraction and chip tandem mass spectrum detection are achieved.

Description

technical field [0001] The invention relates to a three-dimensional electric focusing microfluidic chip for solid-phase microextraction and a manufacturing method thereof. The microfluidic chip can perform solid-phase extraction and be used in conjunction with mass spectrometry to generate Tiny droplets. Background technique [0002] Solid-phase microextraction is a sample processing method first proposed and developed by Arthur and Pawliszy in the early 1990s to absorb and concentrate the target substances in the analytes. SPME is a solvent-free sample pretreatment technology that integrates sampling, extraction, concentration, and sample injection. It is developed on the basis of solid phase extraction technology. Because the volume of its stationary phase is much smaller than the sample volume, it is defined as microextraction. . This technique extracts the target analyte from the sample matrix based on the difference in the partition coefficient of the analyte to be me...

AI Technical Summary

Problems solved by technology

The traditional spray needle ion source needs to pre-treat the sample on other platforms, the sample consumption is large, the separation efficiency and transmission efficiency are not high, etc.

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