Application of rabdocetsin in preparing product for inhibiting proliferation of esophageal squamous carcinoma cells

A technology of fenugreek B and esophageal squamous cell carcinoma, which is applied in the field of biomedicine and can solve the problems of poor prognosis of ESCC and the like

Active Publication Date: 2017-09-29
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cancer diagnosis and treatment technology has made gre

Method used

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  • Application of rabdocetsin in preparing product for inhibiting proliferation of esophageal squamous carcinoma cells
  • Application of rabdocetsin in preparing product for inhibiting proliferation of esophageal squamous carcinoma cells
  • Application of rabdocetsin in preparing product for inhibiting proliferation of esophageal squamous carcinoma cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, preparation Rabdocoestin B (Rabdocoestin B)

[0061]

[0062] The structural formula of saponin B is shown in formula (I), and the steps for preparing saponin B are as follows:

[0063] 1. Pulverize the aerial part of 6Kg of commercially available S. chinensis (sieve mesh: 8 orders) to obtain raw materials with a particle size less than 0.25cm.

[0064] 2. After completing step 1, take the raw material, add 20L of 70% (v / v) acetone aqueous solution for soaking, and soak for three times, each soaking time is 3 days, and soaking solution 1, soaking solution 2 and soaking solution 3 are obtained respectively.

[0065] 3. After step 2 is completed, soaking solution 1, soaking solution 2 and soaking solution 3 are distilled under reduced pressure respectively (for the purpose of recovering acetone), and extracting solution 1, extracting solution 2 and extracting solution 3 are obtained in sequence.

[0066] 4. After completing step 3, add ethyl acetate to e...

Embodiment 2

[0077] Embodiment 2, Cytotoxicity of esophageal squamous cell carcinoma cells of esophageal caraway

[0078] 1. Carawayin B inhibits the proliferation of esophageal squamous cell carcinoma cells

[0079] 1. Culture cells (KYSE30 cells or KYSE450 cells) with modified RPMI-1640 medium containing 10% (v / v) fetal bovine serum, 1% (100U / mL) penicillin and 1% (100 μg / mL) streptomycin ) to the logarithmic growth phase, then digested and resuspended to obtain a concentration of 2×10 4 cells / mL of cell suspension.

[0080] 2. Take a 96-well cell culture plate, add 100 μL of the cell suspension obtained in step 1 to each well (about 2000 cells per well), and change the medium after the cells adhere to the wall to 100 μL of saponin B solution (Xiangcha Vegetable B vegetable solution 1, fine cone fragrant tea vegetable B solution 2, fine cone fragrant tea vegetable B solution 3, fine cone fragrant tea vegetable B solution 4, fine cone fragrant tea vegetable B solution 5, fine cone fragr...

Embodiment 3

[0094] Example 3, Carinadin B inhibits the DNA repair of esophageal squamous cell carcinoma cells

[0095] 1. Cyclone B affects the cycle progression of esophageal squamous cell carcinoma cells

[0096] The experiment was repeated three times to take the average value, and the steps for each repetition were as follows:

[0097] 1. Culture cells (KYSE30 cells or KYSE450 cells) with modified RPMI-1640 medium containing 10% (v / v) fetal bovine serum, 1% (100U / mL) penicillin and 1% (100 μg / mL) streptomycin ) to the logarithmic growth phase, and then digested and resuspended to obtain a concentration of 5×10 4 cells / mL of cell suspension. Take a petri dish (6 cm in size) and add 4 mL of cell suspension (that is, each petri dish is covered with 2×10 5 cells), 5% CO 2 , Cultivate at 37°C for 12h.

[0098] 2. After completing step 1, take the petri dish, discard the liquid phase, and add 4 mL of serum-free culture medium 1640, 5% CO 2 , Culture at 37°C for 12h (starvation culture...

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Abstract

The invention discloses application of rabdocetsin in preparing a product for inhibiting proliferation of esophageal squamous carcinoma cells. Proofed by experiment, the application has the advantages that the rabdocetsin can effectively inhibit the proliferation of the esophageal squamous carcinoma cells, the cloning formation of the esophageal squamous carcinoma cells, affecting the cycle progress of the esophageal squamous carcinoma cells, inducing the retardation of the cycle of the esophageal squamous carcinoma cells, inhibiting the DNA (deoxyribonucleic acid) restoration of the esophageal squamous carcinoma cells, inducing the withering of the esophageal squamous carcinoma cells, regulating up the expression amount of cleaved-caspase-9, regulating up the expression amount of cleaved-caspase-3, regulating up the expression amount of cleaved-PARP, inhibiting Akt signal pathways, inhibiting NF-kappa B signal pathways, and inhibiting the growth of tumors caused by the esophageal squamous carcinoma cells; the important application value is realized.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a new application of asparagusin, and more specifically to the application of asparagusin in the preparation of products for inhibiting the proliferation of esophageal squamous cell carcinoma cells. Background technique [0002] Esophageal squamous cell carcinoma (ESCC) is a very malignant cancer with a high incidence in Asia, and the five-year survival rate is only 15%-25%. Although the technology of cancer diagnosis and treatment has made great progress in recent years, the prognosis of ESCC is still poor. Chemotherapy is the main treatment for patients with advanced ESCC, but most patients are not sensitive to treatment. Therefore, there is an urgent need to develop new drugs with high efficiency and low toxicity. [0003] Plants of the genus Isodon in the Labiatae family are widely used Chinese herbal medicines in my country. The whole plant or root of this genus is used as medic...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00C07D493/10
CPCA61K31/352C07D493/10
Inventor 赫捷普建新陈照丽孙汉董王婧男吴海燕
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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