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Composition containing catalase and application thereof

A catalase and composition technology, applied in the field of medicine, can solve the problems of ineffective radiation particle treatment, inability to repair DNA damage, uneven distribution and the like

Active Publication Date: 2017-09-29
北京法伯新天医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method will permanently leave the carrier particles in the patient's body, which will have a long-term impact on the patient's body. This implantation method also has problems such as uneven distribution and poor coverage, and it is difficult to achieve the desired effect.
[0003] On the other hand, due to the disordered growth of tumors and insufficient angiogenesis, most human solid tumors have oxygen deficiency problems. The ability of tumor cells in a hypoxic state to resist ionizing radiation is several times higher than that of tumor cells with normal oxygen content. The reason is that the damage of oxygen free radicals to DNA is reduced, so radiation has limited lethality to hypoxic tumor cells, resulting in the ineffective therapeutic effect of existing radiation particles
One type of solution in the prior art is to increase the dissolved oxygen concentration in the body by means of a hyperbaric oxygen chamber; another type of method usually uses hypoxic cell sensitizers to reduce the dose of radiation or enhance the effect of radiotherapy, but radiosensitization Drugs are usually highly toxic and will cause adverse effects on the patient's body
For example, a class of nitroaromatic compounds with electrophilic active structures can participate in the bioreductive metabolism of hypoxic cells, weaken the simulation effect of nitro on oxygen, and can fix DNA damage under hypoxic conditions so that DNA damage cannot be repaired. Due to its large side effects, it is greatly restricted in clinical use

Method used

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  • Composition containing catalase and application thereof
  • Composition containing catalase and application thereof
  • Composition containing catalase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare sodium alginate solution. 50 mg of sodium alginate was weighed and dissolved in 10 ml of deionized water, and the sodium alginate was fully dissolved by shaking sufficiently to obtain a sodium alginate solution of 5 mg per ml.

[0026] Prepare iodine-131-labeled catalase. Mix 50 microliters of iodine-131 stock solution and 100 microliters of 0.1 mg per milliliter chloramine-T solution and add 500 microliters of PBS or saline, then add the mixture of iodine-131 and chloramine-T to 500 In human catalase with a concentration of 5 mg / mL in microliters, shake and mix on a vortex shaker for 5 minutes, let stand at room temperature for 15 minutes, and transfer the mixture to a 15 mL ultrafiltration tube after 15 minutes at 4000 Centrifuge for 15 minutes, add dispersion medium to wash 3 times, take out the supernatant, and measure its activity with an activity meter.

[0027] Mix the sodium alginate solution with iodine-131 labeled catalase to obtain a composition con...

Embodiment 2

[0029] Prepare sodium alginate solution. 50 mg of sodium alginate was weighed and dissolved in 10 ml of deionized water, and the sodium alginate was fully dissolved by shaking sufficiently to obtain a sodium alginate solution of 5 mg per ml.

[0030] Preparation of iodine-131 labeled human serum albumin. Mix 50 microliters of iodine-131 stock solution and 100 microliters of 0.1 mg per milliliter of chloramine-T and add 500 microliters of dispersion medium, then add 500 microliters of the mixture of iodine-131 and chloramine-T to a concentration of 5 In milligrams per milliliter of human serum albumin, after shaking and mixing on a vortex shaker for 5 minutes, let it stand at room temperature for 15 minutes, and after 15 minutes, transfer the mixture to a 15 milliliter ultrafiltration tube and centrifuge at 4000 rpm for 15 minutes. After adding dispersion medium and washing for 3 times, the supernatant was taken out, and its activity was measured with an activity meter.

[00...

Embodiment 3

[0033] Prepare potassium alginate solution. 50 mg of potassium alginate was weighed and dissolved in 10 ml of deionized water, and the potassium alginate was fully dissolved by shaking sufficiently to obtain a potassium alginate solution of 5 mg per ml.

[0034] Prepare iodine-125-labeled catalase. Mix 550 microliters of iodine-125 stock solution and 100 microliters of 0.1 mg per milliliter of chloramine-T and add 500 microliters of dispersion medium, then add 500 microliters of the mixture of iodine-125 and chloramine-T to a concentration of 5 In milligrams of catalase per milliliter, after shaking and mixing on a vortex shaker for 5 minutes, let it stand at room temperature for 15 minutes, and after 15 minutes, transfer the mixed solution to a 15 milliliter ultrafiltration tube and centrifuge at 4000 rpm for 15 minutes. After adding dispersion medium and washing for 3 times, the supernatant was taken out, and its activity was measured with an activity meter.

[0035] Potas...

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PUM

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Abstract

The invention discloses a composition containing catalase and an application thereof. The composition comprises radionuclide, soluble alginate and catalase labeled on a biological macromolecule. The composition can be injected into a tumor by means of interventional therapy. When alginate ions in the composition enter the tumor to encounter calcium ions, a gel is formed, so that radionuclide and catalase are uniformly defined in the tumor. According to the composition containing catalase disclosed by the invention, the anoxic state of the tumor cell is improved by decomposing dissolved oxygen generated by hydrogen peroxide in the tumor by means of catalase, and meanwhile, the tumor cells after anoxic improvement are killed by means of radioactive rays, so that the composition has a good tumor treating application prospect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a composition for preparing antitumor agents and its application. Background technique [0002] Radionuclides have different enrichment effects in different organs and tissues. For example, thyroid cells have a special affinity for iodide. After a certain amount of iodine-131 is taken orally, it can be absorbed by the thyroid gland in large quantities. Iodine-131 decays into xenon- At 131, it can emit β-rays (99%) and γ-rays (1%). The effective range of the former is 3.63mm, and the average range is 0.48mm. It can selectively destroy the thyroid acinar epithelium without affecting adjacent tissues. But the enrichment effect also limits the application of radionuclides in other tissues. In the prior art, minimally invasive implantation therapy by radiating particles is currently one of the more ideal methods. Radioactive materials are placed in microcarriers, and radioactive see...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/08A61K51/06A61K38/44A61K47/36A61P35/00A61K101/02
CPCA61K38/443A61K47/36A61K51/06A61K51/08C12Y111/01006A61K2300/00C12N9/0065A61K31/734A61P35/00A61K9/0019A61K9/1652A61K9/1658A61K45/06
Inventor 刘庄杨凯巢宇
Owner 北京法伯新天医药科技有限公司
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