Polypeptide with memory improving effect as well as separation preparation method and application thereof

A technology of inhibiting activity and reversed-phase high-performance liquid phase, which is applied in the preparation methods of peptides, medical preparations containing active ingredients, biochemical equipment and methods, etc. Esterase inhibitory ability, excellent activity, low price effect

Active Publication Date: 2017-10-03
广东华肽生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to overcome the existing difficulties in the preparation of pure polypeptides for improving memory, the primary purpose of the present invention is to provide a polypeptide with memory-improving functions, whose amino acid sequence is Tyr-Asn-Glu

Method used

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  • Polypeptide with memory improving effect as well as separation preparation method and application thereof
  • Polypeptide with memory improving effect as well as separation preparation method and application thereof
  • Polypeptide with memory improving effect as well as separation preparation method and application thereof

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Embodiment

[0043] The separation and preparation method of the polypeptide of the present invention comprises the following steps:

[0044](1) Remove the head and viscera of anchovies, clean them, and put them into a blender to make minced meat; add water twice the weight of the minced meat, and add protease at 0.5% of the mass of the minced meat (based on the quality of the minced fish, the amount of papain is The addition amount accounts for 0.25%, the addition amount of Alcalase 2.4L accounts for 0.25%), incubating enzymolysis at 45°C for 6h, then heating at 95°C for 15min to inactivate the enzyme, cooling to room temperature, centrifuging at 3500r / min for 10min, Collecting the supernatant to obtain an anchovy protein enzymatic hydrolyzate;

[0045] (2) Separation and purification of anchovy protein enzymatic hydrolyzate with Sephadex G-25 gel column, elution with deionized water at a flow rate of 0.5mL / min, detection wavelength is 220nm, a total of 6 components were collected and elu...

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Abstract

The invention discloses a polypeptide with a memory improving effect as well as a separation preparation method and application thereof. An amino acid sequence of the polypeptide is Tyr-Asn-Glu; the separation preparation method of the polypeptide comprises the following steps: adding a mixed enzyme into anchovy and carrying out enzymolysis to obtain enzymolysis liquid; sending the enzymolysis liquid into a Sephadex G-25 gel column and eluting; determining the inhibiting activity of a corresponding component acetylcholinesterase at each eluting peak; further purifying the component with the highest activity by adopting a reversed-phase high performance liquid chromatography to obtain the component with the highest inhibiting activity on the acetylcholinesteras at each eluting peak, namely the polypeptide disclosed by the invention. A protein peptide with the remarkable memory improving effect is prepared by adopting a biological enzymolysis technology and a chromatographic separation technology; the preparation method is simple and feasible, and a prepared product has high purity.

Description

technical field [0001] The invention belongs to the field of protein deep processing, and in particular relates to a polypeptide with memory-improving function, its separation and preparation method and application. Background technique [0002] Studies have shown that memory decline or degeneration is related to lesions in the hippocampus, neurotransmitter reduction, and synaptic lesions. In addition, oxidative stress in the central nervous system (Central nervous system, CNS) will increase with age, leading to many age-related neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, dementia, multiple sclerosis, etc. It is estimated that the number of patients will increase to 115 million by 2050. [0003] The loss of acetylcholine, a neurotransmitter in the human brain, is one of the important factors leading to memory impairment. Acetylcholine is an important neurotransmitter in human nerve cells, which can transmit nerve signals between synapses....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/087C12P21/06C07K1/20C07K1/16A23L17/20A23L33/18A61K38/06A61P25/28
CPCA23L17/20A23L33/18A23V2002/00A61K38/00C07K5/0812C12N9/18C12P21/06C12Y301/01007A23V2200/322
Inventor 赵谋明苏国万赵诩君
Owner 广东华肽生物科技有限公司
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