Method for preparing high-purity polyfructose through simulated moving bed chromatography

A technology for simulating moving bed and polyfructose is applied in the field of polyfructose preparation, which can solve the problem of low purity of polyfructose, and achieve the effects of realizing continuous operation, reducing production cost and improving utilization rate.

Inactive Publication Date: 2017-10-13
深圳市华利康纤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a method for preparing high-purity polyfructose by using simulated moving bed chromatography technology, so as to solve the problem of low purity of polyfructose in the prior art

Method used

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  • Method for preparing high-purity polyfructose through simulated moving bed chromatography
  • Method for preparing high-purity polyfructose through simulated moving bed chromatography
  • Method for preparing high-purity polyfructose through simulated moving bed chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: Preparation of inulin refined liquid.

[0025] Get 500g fresh chicory clean, peel, grind and crush; The chicory of crushing is added in the Tris-HCl buffer solution that contains pectinase, the concentration of described Tris-HCl buffer solution is 1mol / L, and pH is 6.8, fruit The amount of glue enzyme added is 1000U / L, stirred at 35 degrees Celsius for 3 hours, then heated to 100 degrees Celsius, and stirred for 5 hours; filtered the inulin extract to obtain a crude inulin extract;

[0026] Carry out decolorization, debittering, and desalination treatment to the inulin crude extract, pass the inulin crude extract through activated carbon, chitosan, cationic macroporous resin, and nanofiltration membrane successively, the cationic macroporous resin is H103, and the The nanofiltration membrane used is DL8040F to obtain the inulin refined solution.

Embodiment 2

[0028] A method utilizing simulated moving bed chromatography to prepare high-purity polyfructose, the method comprising the steps of:

[0029] The inulin refined liquid is added to a simulated moving bed chromatographic separation device for separation and purification, using water as the eluent. The simulated moving bed includes areas I, II, III, and IV, and each area includes 6 chromatographic columns. Among them, the chromatographic columns in zone Ⅰ and zone Ⅱ are filled with IR-120 strong acid cation exchange resin, and the chromatographic columns in zone Ⅲ and zone Ⅳ are filled with Y-type molecular sieves;

[0030] The process parameters of separation and purification are as follows:

[0031] The operating temperature is 45°C, the pressure is 0.15Mpa, the flow rate of the feed liquid is 50mL / min, and the flow rate of the eluent is 50mL / min. It is 55cm / h, and the flow velocity in IV district is 14cm / h; The obtained polyfructose effluent solution is evaporated and conce...

Embodiment 3

[0033] Other conditions were the same as in Example 2, except that the operating temperature of the simulated moving bed chromatographic separation device was adjusted to 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, and 60°C. Detect the polyfructose content and degree of polymerization at the polyfructose outflow port, and the test results are shown in the table below.

[0034] Table 1 The influence of temperature on the separation efficiency of polyfructose

[0035]

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Abstract

The invention discloses a method for preparing high-purity polyfructose through simulated moving bed chromatography. The method comprises the following steps: (1) performing decolorization, debittering and desalting treatment on an inulin crude extraction solution to obtain an inulin refined solution; (2) adding the inulin refined solution obtained in the step (1) into a simulated moving bed chromatographic separation device, and performing separation and purification by using water as an eluting agent, wherein the simulated moving bed comprises regions I-IV, each region comprises 1-6 chromatographic columns, the chromatographic columns of the region I and the region II are filled with strongly acidic cation exchange resin, and the chromatographic columns of the region III and the region IV are filled with cation molecular sieves; and (3) performing reduced-pressure evaporation and concentration on the obtained polyfructose solution to obtain the high-purity polyfructose. According to the invention, the purity of the obtained polyfructose is high, the total column efficiency is high, the consumption of a mobile phase is low, continuous operation can be realized, the utilization ratio of raw materials is improved, and the production cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of polyfructose preparation, and in particular relates to a method for preparing high-purity polyfructose by using simulated moving bed chromatography technology. Background technique [0002] Chicory is a perennial herb of the genus Chicory in the Asteraceae family, also known as European chicory, coffee grass or French endive. It is native to Europe, West Asia, Central Asia and North America, and is distributed in Northwest my country, North China and Northeast China. Juba has high nutritional value, resistance to diseases and insect pests, high yield, and strong regeneration ability. It is a high-yield and high-quality forage with great development potential. The fleshy root of chicory contains about 70% (dry matter weight) of inulin, which can be used as raw material for the production of inulin, fructooligosaccharide (FOS) and high fructose syrup. [0003] The fructosyl series products refer to the pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/18
CPCC08B37/0003C08B37/0054
Inventor 杨云黄振华赵国萍王小红
Owner 深圳市华利康纤生物科技有限公司
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