Soil heavy metal remedying biological improver and preparation method thereof

A bio-improving agent and heavy metal technology, applied in soil conditioning materials, chemical instruments and methods, fertilizer mixtures, etc., can solve the problems of ineffective remediation of heavy metal pollution, difficulty in ensuring the growth and reproduction of microorganisms, poor improvement effect of soil amendments, etc. , to achieve the effect of improving soil physical and chemical properties, suitable for large-scale production, and improving soil microbial environment

Active Publication Date: 2017-10-17
北京世纪阿姆斯生物工程有限公司 +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the remediation technologies for heavy metal-contaminated soil in related technologies mainly include: physical remediation, chemical remediation, and biological remediation. Among them, biological remediation is a research hotspot in recent years, but the bottleneck problem of "microorganisms are afraid of encountering heavy metals" has long restricted microbial remediation. The development and use of related technologies are difficult to ensure the smooth growth and reproduction of microorganisms in harsh environments polluted by heavy metals
In addition, the improvement effect of the soil conditioner used in related technologies is not good, the scope of improvement is not comprehensive, and it is easy to cause negative problems such as secondary pollution of the soil, and the repair effect on heavy metal pollution is not obvious

Method used

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  • Soil heavy metal remedying biological improver and preparation method thereof
  • Soil heavy metal remedying biological improver and preparation method thereof
  • Soil heavy metal remedying biological improver and preparation method thereof

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preparation example Construction

[0061] The preparation method of the soil heavy metal remediation bio-improvement agent of the present invention has simple process and is suitable for large-scale production.

[0062] In a preferred embodiment of the present invention, the preparation method of the microbial inoculum comprises:

[0063] Carrying out liquid culture of the strains of the microbial bacterial agent to obtain a fermentation broth;

[0064] Centrifuge to collect the sludge;

[0065] The obtained fungal slime is fully mixed with diatomaceous earth to obtain the microbial bacterial agent.

[0066] In a preferred embodiment of the present invention, the number of effective viable bacteria contained in each milliliter of the fermented liquid is 1×10 11 More than one to ensure the effect of microorganisms in the targeted passivation of heavy metal ions in the environment.

[0067] In a preferred embodiment of the present invention, the rotational speed of the centrifuge is above 3000 rpm, preferably ...

Embodiment 1

[0092] A preparation method of a soil heavy metal remediation bio-improvement agent, comprising the steps of:

[0093] 1. The Bacillus subtilis is subjected to liquid high-density culture to obtain a fermented liquid, and it is ensured that the number of effective viable bacteria in each milliliter of fermented liquid is greater than 1×10 11 indivual;

[0094] 2. Carry out high-speed centrifugation on the above-mentioned fermented liquid through a pre-configured centrifuge to collect the sludge, wherein the speed of the centrifuge is 3000rpm, and the centrifugation time is 5min;

[0095] 3. Mix and absorb the collected bacteria slime and pre-configured diatomite according to the mass ratio of bacteria sludge: diatomite = 1:10, dry at 70°C for 6 hours after adsorption, pulverize, pass through a 100-mesh sieve, and make into Bacillus subtilis powder;

[0096] 4. Pyrolyze wheat straw at 350°C under anaerobic conditions for 4 hours to obtain biochar for later use;

[0097] 5. W...

Embodiment 2

[0099] A preparation method of a soil heavy metal remediation bio-improvement agent, comprising the steps of:

[0100] 1. The Bacillus subtilis and Bacillus amyloliquefaciens are respectively subjected to liquid high-density culture to obtain a fermentation broth, and ensure that the number of effective viable bacteria per milliliter of fermentation broth is greater than 1×10 11 indivual;

[0101] 2. Carry out high-speed centrifugation on the above-mentioned fermented liquid through a pre-configured centrifuge to collect the sludge, wherein the rotating speed of the centrifuge is 5000rpm, and the time of the centrifugation is 3min;

[0102] 3. Mix and absorb the collected bacteria slime and pre-configured diatomite according to the mass ratio of bacteria sludge: diatomite = 1:50, dry at 80°C for 2 hours after adsorption, pulverize, pass through a 100-mesh sieve, and make into a single-function bacterial powder;

[0103] 4. Mix Bacillus subtilis powder and Bacillus amylolique...

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Abstract

The invention provides a soil heavy metal remedying biological improver and a preparation method thereof. The soil heavy metal remedying biological improver is obtained by compounding a microbial inoculum, biomass carbon, organosilicone, a nitrogen raw material, quick-acting calcium, a phosphate ore powder and amino acid according to specific proportions. The soil heavy metal remedying biological improver can effectively remedy heavy metal pollution to soil, improve physical and chemical properties of the soil, improve the microbial environment of the soil, balance soil nutrients and improve soil fertility through biological means, and can promote crop growth, so that the grain yield is increased and the grain quality is improved. The preparation method of the soil heavy metal remedying biological improver is simple in process, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of biological soil improvers, in particular to a soil heavy metal remediation biological improver and a preparation method thereof. Background technique [0002] Heavy metal pollution is easy to accumulate in the environment and difficult to degrade. After being absorbed by crops, it inhibits crop growth, reduces yield, and ultimately endangers human health through the transmission of food chains. There are nearly 20 million hectares of heavy metal-contaminated cultivated land in my country, and 12 million tons of heavy metal-contaminated grain per year, seriously affecting the quality and safety of my country's agricultural products. [0003] At present, the remediation technologies for heavy metal-contaminated soil in related technologies mainly include: physical remediation, chemical remediation, and biological remediation. Among them, biological remediation has been a research hotspot in recent years, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C09K101/00
CPCC09K17/40C09K2101/00
Inventor 吴书凤邓祖科李艳萍李伏明周士龙孔令波袁梅张敏陈劲伊
Owner 北京世纪阿姆斯生物工程有限公司
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