A kind of preparation method of microbial carrier

A microbial carrier, a technology of preparation steps, applied in the directions of immobilization on or in inorganic carriers, chemical instruments and methods, phosphorus compounds, etc., can solve the problems of poor biocompatibility, low microbial load, low mechanical strength, etc. The effect of increasing the space for attachment, increasing the load, and improving the survival rate

Active Publication Date: 2021-01-19
上海同柏宸科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention aims at the defects of low mechanical strength, poor biocompatibility and low microbial load in common microbial immobilization carriers at present, and provides a preparation method of microbial carriers

Method used

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  • A kind of preparation method of microbial carrier

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Effect test

example 1

[0025] Weigh the oyster shells and put them in water, heat and boil for 1 hour, take out the boiled oyster shells and put them into a jet mill to grind for 30 minutes to obtain oyster shell powder, then mix the oyster shell powder with a concentration of 0.1mol / L according to the mass ratio of 1:5 After mixing the hydrochloric acid, stir and react for 1 hour, then centrifuge with a centrifuge at 2000r / min for 10 minutes to separate the supernatant; weigh the cotton stalks and cut them into small pieces with a length of 1 cm to obtain cotton stalks, and mix the cotton stalks and biogas slurry by The mass ratio is 1:3, mixed and placed under the condition of temperature of 35°C, and left to be slightly rotted for 3 days. Mix the powder and absolute ethanol at a mass ratio of 1:10, heat up to 40°C, stir and react for 1 hour to obtain a mixed suspension, and then add phosphorus pentoxide and the mixed suspension to the ultrasonic dispersion at a mass ratio of 1:15 In the instrumen...

example 2

[0027] Weigh the oyster shells and put them in water to heat and boil for 2 hours, take out the boiled oyster shells and put them into a jet mill to grind for 35 minutes to obtain oyster shell powder. After mixing the hydrochloric acid, stir and react for 2 hours, and centrifuge at 2500r / min for 13 minutes to separate the supernatant; weigh the cotton stalks and cut them into small pieces with a length of 1 cm to obtain cotton stalks. The mass ratio is 1:3, mixed and placed under the condition of temperature of 40°C, and left to be slightly rotted for 4 days. Mix the powder and absolute ethanol at a mass ratio of 1:10, heat up to 45°C, stir and react for 2 hours to obtain a mixed suspension, and then add phosphorus pentoxide and the mixed suspension to the ultrasonic dispersion at a mass ratio of 1:15 In the instrument, ultrasonic dispersion treatment was performed for 35 minutes at a frequency of 35 kHz to obtain a dispersion liquid, and then the pH of the dispersion liquid w...

example 3

[0029] Weigh the oyster shells, put them in water, heat and boil for 2 hours, take out the boiled oyster shells, put them into a jet mill and pulverize them for 40 minutes to obtain oyster shell powder, and then mix the oyster shell powder with a concentration of 0.1mol / L according to the mass ratio of 1:5 After mixing the hydrochloric acid, stir and react for 2 hours, and centrifuge at 3000r / min for 15 minutes to separate the supernatant; weigh the cotton stalks and cut them into small pieces with a length of 2cm to obtain cotton stalks. The mass ratio is 1:3, mixed and placed under the condition of temperature of 45°C, and left to be slightly rotted for 5 days. Mix the powder and absolute ethanol at a mass ratio of 1:10, heat up to 50°C, stir and react for 2 hours to obtain a mixed suspension, and then add phosphorus pentoxide and the mixed suspension to the ultrasonic dispersion at a mass ratio of 1:15 In the instrument, under the condition of frequency of 40kHz, the ultras...

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Abstract

The invention relates to the technical field of microbial immobilization, in particular to a preparation method for a microbial carrier. A porous cotton straw is used as a template, EDTA (Ethylene Diamine Tetraacetic Acid) is taken as a guiding agent to improve the purity of hydroxy calcium phosphate, a porous high-capacity hydroxy calcium phosphate carrier is prepared, mosses are loaded on the surface of the porous high-capacity hydroxy calcium phosphate carrier to provide places and nutrition for the growth of microorganisms, and good mechanical performance and excellent biocompatibility of the hydroxy calcium phosphate are used for finally preparing the microbial carrier with high biological capacity. The defects of poor mechanical performance, low biocompatibility and small biological capacity of a common microbial carrier are eliminated, and the microbial carrier with the high biological capacity has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganism immobilization, in particular to a method for preparing a microorganism carrier. Background technique [0002] Since the advent of immobilized microorganism technology, it has shown broad development prospects. Immobilized microorganisms have achieved remarkable results in the ethanol fermentation industry. Compared with traditional fermentation processes, immobilized yeasts are used to ferment alcohol with fast speed, short cycle, high production capacity, simple process equipment, and easy to achieve continuous and automatic production. It has the advantages of easy extraction, etc., and reduces the equipment investment and environmental pollution of fermentation factories to a certain extent. [0003] Selecting a suitable immobilized microbial carrier is the key to industrialization, and the quality of the carrier depends on the mechanical strength, lifespan, capacity and activity of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C01B25/32
CPCC01B25/32C12N11/14
Inventor 陈军林茂兰
Owner 上海同柏宸科生物技术有限公司
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