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Detection method for sesquiterpene component in radix curcumae

A detection method, the technology of Wenyujin, which is applied in the field of detecting the content of volatile oil active ingredients in Wenyujin, a traditional Chinese medicine, can solve the problems of environmental hazards for experimental operators, low polarity of sesquiterpenoids, and easy contamination of chromatographic columns, etc., to improve the environment. The effect of protection benefit, good chemical stability and thermal stability, safe and reliable experimental environment

Inactive Publication Date: 2017-10-20
ZHEJIANG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low polarity of sesquiterpenoids, reversed-phase column separation often requires long analysis time and low separation efficiency, and the chromatographic column is easily contaminated, and the pollutants deposited on the column appear in the form of impurity peaks, which affect the analysis results
In addition, a large amount of toxic organic solvents are used in the experiment process, which will cause certain harm to the experiment operators and the environment.

Method used

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  • Detection method for sesquiterpene component in radix curcumae
  • Detection method for sesquiterpene component in radix curcumae
  • Detection method for sesquiterpene component in radix curcumae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The selection of embodiment 1 adsorbent

[0057] Weigh 200 mg of 9 portions of curcuma curcuma powder in parallel and add them to 5 groups of mortars respectively, and weigh different kinds of adsorbents (molecular sieve TS-1, diatomaceous earth, neutral Al 2 o 3 , silicone, C 18 , molecular sieve MCM-48, molecular sieve SBA-15, molecular sieve SAPO-11, molecular sieve ZCM-5) each 200mg, respectively added to 9 groups of mortars and grinded with Wenyujin medicinal material powder for 150s. Take nine 3mL solid-phase extraction columns, add sieve plates to the bottom, fill the extraction columns with ground solids, and add sieve plates to the top after filling the packing. Take 1.0mL of methanol, inject it into the extraction column, use a syringe to properly pressurize the extraction column, and use a 1.5mL centrifuge tube to collect the eluate at the bottom. After the elution, evaporate the eluate in the centrifuge tube to dry methanol. Reconstitute with 250 μL elect...

Embodiment 2

[0065] The selection of embodiment 2 adsorbent consumption

[0066] Weigh in parallel 5 copies of Curcuma curcuma medicinal material powder, 200mg each, and add them to 5 groups of mortars, and weigh molecular sieve TS-1 with different masses (50mg, 100mg, 200mg, 300mg, 400mg), add them to 5 groups of mortars and mortars respectively. Powder grinding for 150s. Take 5 3mL solid-phase extraction columns, add sieve plates to the bottom, fill the extraction columns with ground solids, and add sieve plates to the top after filling the packing. Take 1.0mL of methanol, inject it into the extraction column, use a syringe to properly pressurize the extraction column, and use a 1.5mL centrifuge tube to collect the eluate at the bottom. After the elution, evaporate the eluate in the centrifuge tube to dry methanol. Redissolve with 250 μ L of electrophoresis running buffer, filter with a 0.22 μm filter membrane, and detect and analyze with a high-efficiency capillary electrophoresis inst...

Embodiment 3

[0071] Embodiment 3 grinding time selection

[0072] Weigh 4 parts of Curcuma curcuma medicinal material powder in parallel and add 200 mg each into 4 groups of mortars, and weigh 4 parts of 200 mg molecular sieve TS-1, add them into 4 groups of mortars respectively and grind them for different times (90s, 120s, 150s , 180s). Take 4 solid phase extraction columns of 3mL size, add sieve plates to the bottom, fill the extraction columns with ground solids respectively, and add sieve plates to the top after filling the packing. Take 1.0mL of methanol, inject it into the extraction column, use a syringe to properly pressurize the extraction column, and use a 1.5mL centrifuge tube to collect the eluate at the bottom. After the elution, evaporate the eluate in the centrifuge tube to dry methanol. Reconstitute with 250 μL of electrophoresis running buffer, filter through a 0.22 μm filter membrane, and detect and analyze with a high-performance capillary electrophoresis (HPCE), as in...

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Abstract

The invention discloses a detection method for a sesquiterpene component in radix curcumae. The method comprises the following steps: volatilizing and drying a solvent of a radix curcumae extracting solution and carrying out buffering solution re-dissolution by utilizing electrophoresis; filtering the solution with a 0.22mu m filter membrane and taking filtrate as a sample to be detected; detecting peak area of each effective component in the sample to be detected by adopting a micro-emulsion electrokinetic capillary chromatography; obtaining the content of curdione, curcumol, germacrone, furanodiene and beta-elemene in the sample to be detected of the radix curcumae respectively according to each reference substance standard curve of the curdione, the curcumol, the germacrone, the furanodiene and the beta-elemene. The invention innovatively provides a micro-extraction detection method of volatile oil type components in traditional Chinese medicines. According to the method disclosed by the invention, a matrix solid-phase dispersion and extraction technology taking a molecular sieve as an adsorbent is combined with a micro-emulsion electrokinetic capillary chromatography technology, so that the content of the volatile oil type effective components in the medicinal material radix curcumae can be efficiently and environmentally friendly extracted and detected.

Description

(1) Technical field [0001] The invention belongs to the technical field of extraction and analysis of natural medicines. It relates to a micro-extraction and detection method for volatile oil components in Curcuma curcuma, specifically, it involves using molecular sieve TS-1 as an adsorbent, combined with micro-matrix solid-phase extraction and dispersion technology and high-efficiency capillary electrochromatography technology, to detect traditional Chinese medicine Curcuma curcuma A new method for the content of active ingredients in volatile oils. (2) Background technology [0002] Wen Yujin (Curcuma wenyujin Y.H.Chen et C.Ling) is a plant of the genus Curcuma longa in the Zingiberaceae family. It is mainly produced in Ruian, Zhejiang Province. It is mostly cultivated and rarely wild. The 2015 edition of the Chinese Pharmacopoeia recorded three traditional Chinese medicines derived from Wen Yujin, including Wen Yujin, Wen Curcuma and Turmeric. Traditional medicine belie...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/38
CPCG01N30/02G01N30/06G01N30/38G01N2030/062G01N2030/388
Inventor 楚楚魏蒙蒙王珊李清颜继忠
Owner ZHEJIANG UNIV OF TECH
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