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Primers, kits and methods for identifying wax-free non-heading Chinese cabbage

A technology of head cabbage and kit, applied in the field of molecular biology, to achieve the effect of improving efficiency

Active Publication Date: 2020-11-03
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the molecular markers of non-heading Chinese cabbage without wax powder.

Method used

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  • Primers, kits and methods for identifying wax-free non-heading Chinese cabbage
  • Primers, kits and methods for identifying wax-free non-heading Chinese cabbage
  • Primers, kits and methods for identifying wax-free non-heading Chinese cabbage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The technical roadmap of this embodiment is as follows image 3 shown.

[0037] Primer selection

[0038] (1) Parent selection

[0039] The parental material 13S126 (without wax powder) is a green and bright multi-generation self-incompatible line with medium maturity, medium plant height, small plant development, small leaves, long petiole, low yield and good quality; The cross-incompatible parent material 13S106 (with wax powder) is medium ripening, medium plant height, small plant expansion, large leaves, long petiole, high yield, and poor quality.

[0040] (2) Parent and F 1 , F 2 Field phenotyping of offspring

[0041] From 2014 to 2016, two parents, F 1 Bagging and self-crossing were carried out, the seeds were harvested from a single plant and sown into plots, and the phenotypes of the parents and offspring were investigated at the maturity stage. f 2 The wax substitute plants were close to 3:1 with no wax powder plants. in, figure 2 It is the field tr...

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PUM

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Abstract

The invention provides a primer for distinguishing wax-powder-free and non-heading cabbage, a kit and a distinguishing method. The primer is shown as SEQ ID NO.1 and SEQ ID No.2. The invention also provides a kit comprising the above mentioned primer for distinguishing the wax-powder-free and non-heading cabbage. With the adoption of the primer, the wax-powder-free and non-heading cabbage can be effectively distinguished; the theoretical basis and the material source are provided for high-quality high-performance culture of Chinese special vegetable, namely, non-heading cabbage; and the theoretical basis is provided for the high-quality high-performance culture.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a primer, a kit and an identification method for identifying wax-free non-heading Chinese cabbage. Background technique [0002] Vegetable wax means that the layer covering the epidermal cells of plants is composed of a series of lipophilic very-long-chain fatty acids (very-long-chain fatty acids) compounds with more than 18 carbon atoms and mainly composed of 24 to 34 carbon atoms The surface of organs and tissues such as leaves, stems, flowers and fruits of plants is covered with wax. Vegetable waxes are composed of long-chain saturated fatty acids, alcohols, alkanes, aldehydes, ketones and other substances (Kunst and Samuels 2003). The waxy layer of the epidermis distributed on the surface of the plant cuticle is the first barrier for plants to resist external environmental stimuli. It has the functions of preventing non-porous water loss, resisting the invasion of germs and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 万正杰王灿洁杨庆勇夏军辉李艺潇姚培杰刘旭佳
Owner HUAZHONG AGRI UNIV