A kit for detecting pneumococcal serotype 10a

A pneumococcus and serotype technology, applied in the field of PCR detection, can solve problems such as inability to amplify fragments, lack of rapid detection molecular typing assay methods, and inability to amplify specific fragments, etc., to eliminate the influence of subjective factors, experiment Low-cost, easy-to-achieve effects
CN107312862BActive Publication Date: 2021-01-01AB&B BIO TECH CO LTD JS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
AB&B BIO TECH CO LTD JS
Publication Date
2021-01-01

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Abstract

The invention provides a kit for detecting pneumococcus 10A serotype, and belongs to the technical field of PCR (polymerase chain reaction) detection. The kit comprises a specific primer pair with nucleotide sequences shown in SEQ ID NO.1 to 2. The invention also provides a method for detecting the pneumococcus 10A serotype. The kit has the advantages that a to-be-detected sample is performed with PCR detection by the specific primer pair; if an amplified product is 607bp, the to-be-detected sample contains the pneumococcus 10A serotype; according to the kit, the detection is accurate, the sensitivity is high, the specificity is strong, and the detection is simple, convenient and quick; the kit is suitable for performing epidemiological investigation on the pneumococcus, and quick detection and strain identification on bacteria for production of 23-valent pneumococcal polysaccharide vaccines; the application prospect is good.
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Description

technical field

[0001] The invention relates to the technical field of PCR detection, in particular to a kit for detecting pneumococcus 10A serotype and application thereof. Background technique

[0002] Streptococcus pneumoniae is a common human pathogen. It is the most common cause of acquired pneumonia in people of all ages in developing and developed countries. It is also the main pathogen that causes diseases such as otitis media, pneumonia, meningitis and sepsis. Pneumococcus is a Gram-positive bacterium, arranged in single or double balls, without flagella, spores, and capsules, and different types of capsules have different thicknesses. The capsule of pneumococcus is a necessary condition for its virulence, and the capsular polysaccharide has group / type specificity, which is the basis of pneumococcal group / type. So far, pneumococcus can be divided into 46 groups and more than 90 serotypes. The type of pneumococcus that causes disease often varies with regions, ages ...

Claims

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