The invention relates to a PCB double-sided tri-proof paint coating quality detecting system and method, which belongs to the field of coating quality detection. The system comprises an upper computer, a controller, a light source module, an image acquisition module, a carrier, a position detecting module, a position fixing module and a carrier turnover module, wherein the upper computer is connected with the light source module, the image acquisition module, the position detecting module, the position fixing module, the carrier turnover module through the controller; and one or more to-be-detected PCBs are carried on the carrier. The system applies a color index method to divide a coating area, and compares with an MASK image to detect tri-proof paint coating quality. The system is simplein structure, can realize double-sided detection through a plate turnover device, reduces the cost, is high in detecting speed and is high in precision.