Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Collagen/bacterial cellulose composite membrane dressing and preparation method thereof

A technology of bacterial cellulose film and bacterial cellulose, which is applied in the field of medical biomaterials, can solve the problems of reduced biological safety, reduced film performance, and loss of biological activity of dressings, and achieves improved biological safety, improved retention stability, collagen The effect of increased import volume

Active Publication Date: 2020-08-14
WUHAN POLYTECHNIC UNIVERSITY
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the existing collagen / bacterial cellulose composite film dressing (material) manufacturing process, there are still many deficiencies, such as the introduction of toxic chemical reagents in the manufacturing process, resulting in reduced biological safety of the dressing, low collagen import or unstable combination of imported collagen It is difficult to meet the requirements of biological efficacy, extreme conditions in the manufacturing process lead to collagen denaturation and loss of its biological activity, and the manufacturing process destroys the natural network structure of the bacterial cellulose membrane, resulting in a decrease in membrane performance, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagen/bacterial cellulose composite membrane dressing and preparation method thereof
  • Collagen/bacterial cellulose composite membrane dressing and preparation method thereof
  • Collagen/bacterial cellulose composite membrane dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] One aspect of the present invention provides a kind of preparation method of collagen / bacterial cellulose composite film dressing, and this preparation method comprises:

[0030] (1) Purify the natural bacterial cellulose membrane;

[0031] (2) The purified aqueous bacterial cellulose membrane is directly vacuum freeze-dried without pre-freezing treatment to obtain a macroporous bacterial cellulose membrane;

[0032] (3) Under the condition of less than 20°C, the natural collagen is dissolved with aqueous acetic acid to obtain a collagen solution;

[0033] (4) immersing the macroporous bacterial cellulose membrane in the collagen solution, and introducing collagen into the bacterial cellulose membrane under vacuum conditions;

[0034] (5) placing the collagen-introduced bacterial cellulose membrane into a phosphate buffer solution with a pH of 6.0-8.0, and performing fibrillation treatment to obtain a collagen fibrillated composite membrane;

[0035] (6) directly vacu...

Embodiment 1

[0051] Place the bacterial cellulose membrane in a beaker and soak it in enough ultrapure water for 24 hours to make it fully absorb water and swell. Then boil with 0.1mol / L NaOH solution for 30min, wash with ultrapure water until neutral, then transfer the bacterial cellulose membrane to 0.05mol / L acetic acid solution for soaking for 24h, wash with ultrapure water until neutral for later use. The purified aqueous bacterial cellulose membrane is placed in a freeze dryer, vacuumed, and the absolute pressure of the system is reduced to 20Pa within 5 minutes, and the macroporous structure bacterial cellulose membrane (such as Figure 1a and Figure 2a ). Dissolve type I collagen extracted from pig skin with 0.1 mol / L acetic acid aqueous solution at no higher than 20°C to obtain a collagen solution with a concentration of 5 mg / mL. Immerse the bacterial cellulose membrane with macroporous structure in the collagen solution, evacuate to a vacuum degree of 0.09MP to promote the intr...

Embodiment 2

[0053] Place the bacterial cellulose membrane in a beaker and soak it in enough ultrapure water for 24 hours to make it fully absorb water and swell. Then boil with 0.1mol / L NaOH solution for 30min, wash with ultrapure water until neutral, then transfer the bacterial cellulose membrane to 0.05mol / L acetic acid solution for soaking for 24h, wash with ultrapure water until neutral for later use. The purified aqueous bacterial cellulose membrane was placed in a freeze dryer, vacuumed, and the absolute pressure of the system was reduced to 30 Pa within 5 minutes, and the bacterial cellulose membrane with a macroporous structure was obtained after continuing to freeze-dry for 48 hours. Dissolve type I collagen extracted from grass carp skin with 0.1 mol / L acetic acid aqueous solution at no higher than 20°C to obtain a collagen solution with a concentration of 8 mg / mL. Immerse the bacterial cellulose membrane with macroporous structure in the collagen solution, evacuate to a vacuum ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a collagen / bacterial cellulose composite film dressing and a preparation method thereof, comprising: purifying the natural bacterial cellulose film; directly vacuum freezing the purified water-containing bacterial cellulose film without pre-freezing treatment dry to obtain bacterial cellulose membrane with macroporous structure; under the condition of not higher than 20°C, dissolve natural collagen with acetic acid aqueous solution to obtain collagen solution; immerse bacterial cellulose membrane with macroporous structure in collagen solution, and place it under vacuum condition Introduce collagen into the bacterial cellulose membrane; place the bacterial cellulose membrane into which the collagen is pH 6.0-8.0 phosphate buffer solution, carry out fibrosis treatment, obtain the composite membrane of collagen fibrillation; the composite membrane of collagen fibrillation, no After pre-freezing treatment, direct vacuum freeze-drying to obtain collagen / bacterial cellulose composite film dressing. In the present invention, the collagen import amount, the biological stability of the collagen and the retention stability in the membrane are all significantly improved.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and more specifically relates to a collagen / bacterial cellulose composite membrane dressing and a preparation method thereof. Background technique [0002] Medical dressings are widely used in clinical surgical fields such as skin trauma and burn treatment. High-quality medical dressings require good biological safety, barrier properties, water absorption and water retention, and suitable mechanical properties. For higher-end medical dressing products, such as dressings for large area burns, in order to promote the self-healing of skin tissue and reduce the pain of patients during dressing replacement, dressings are often required to have the properties of promoting tissue healing and the dressing itself can be degraded. Therefore, more and more natural macromolecular materials with the above-mentioned biological effects are applied in the manufacture of medical dressings. Among th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/28A61L15/32A61L15/42
CPCA61L15/28A61L15/325A61L15/42C08L1/02
Inventor 汪海波张军涛未本美许玉玲何浪许承志
Owner WUHAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com