Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SSR molecular marker primer of quercus liaotungensis koidz, method for identifying varieties of quercus liaotungensis koidz and application of method

A technology of molecular markers and varieties, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as difficult to distinguish Quercus liaotungen, and achieve efficient and reliable identification

Inactive Publication Date: 2017-12-01
山西省林业科学研究院
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After years of hard work, the Shanxi Academy of Forestry has bred 6 varieties of Quercus liaotungen with excellent performance, but it is difficult to distinguish different varieties of Quercus liaotungung according to their appearance.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SSR molecular marker primer of quercus liaotungensis koidz, method for identifying varieties of quercus liaotungensis koidz and application of method
  • SSR molecular marker primer of quercus liaotungensis koidz, method for identifying varieties of quercus liaotungensis koidz and application of method
  • SSR molecular marker primer of quercus liaotungensis koidz, method for identifying varieties of quercus liaotungensis koidz and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Specific embodiments of the present invention will be described in detail below. It should be understood that the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0026] 1. Materials

[0027] Six species of Quercus liaotungen were numbered A, B, C, D, E and F, provided by the Shanxi Academy of Forestry. The leaves of the samples to be tested were quick-frozen in liquid nitrogen and stored in a -76°C refrigerator for later use. Fluorescently labeled SSR primers were synthesized by Ruibo Xingke Biotechnology Co., Ltd. The sequence information of the primers is shown in Table 1:

[0028] Table 1. PCR primers used for SSR analysis of different species of Quercus liaotungensis

[0029]

[0030] 2. Method

[0031] 2.1 Genomic DNA extraction

[0032] Take about 10g of frozen leaves from the refrigerator, put them in a mortar pre-cooled with liquid nitrogen, add liqui...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a SSR molecular marker primer of Quercus liaotungensis, and the primer comprises ssrQrZAG 96, ssrQrZAG 7, Qden 03011 and Qden 05031. The invention also discloses a method for identifying Quercus liaotungense species by using SSR molecular marker primers and its application. After applying the method for identifying the species of Quercus liaotungen provided by the invention, different species of Quercus liaotungen can be accurately, efficiently and reliably identified.

Description

technical field [0001] The invention relates to an SSR molecular marker primer of Quercus liaotungensis, a method for identifying the species of Quercus liaotungungen and its application. Background technique [0002] Quercus liaotungensis Koidz is a plant of the genus Quercus in the family Fagaceae. It is a deciduous tree with a height of 15m; Sleepers, furniture, etc. At present, Quercus liaotungen has been widely used in afforestation of barren hills in Shanxi. Therefore, it is of great significance to select good varieties of Quercus liaotungung for seedling cultivation. After years of hard work, Shanxi Academy of Forestry has bred 6 varieties of Quercus liaotungen with excellent performance, but it is difficult to distinguish different varieties of Quercus liaotungen according to their appearance. This application proposes a method for distinguishing different varieties of Quercus liaotungensis using SSR molecular markers, and the result is accurate, efficient and rel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156
Inventor 郭斌杨欣超王建义马佳琳
Owner 山西省林业科学研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com