Method for obtaining metabolic difference between genetically modified and non-genetically modified maize based on UHPLC-MS (Ultra High Performance Liquid Chromatography-Mass Spectrometer)

A transgenic corn, non-transgenic technology, applied in the field of analytical chemistry, can solve the problems of low accuracy of liquid mass analysis platform, less compound information, easy to introduce errors, etc., to achieve efficient and reliable metabolite information identification, high detection resolution, elimination of the effect of interference

Active Publication Date: 2019-04-30
黑龙江省农业科学院农产品质量安全研究所
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Problems solved by technology

However, at present, due to the multi-step pretreatment process, it is easy to introduce errors in the sample preparation process; the liquid mass analysis platform has low accuracy, and the operation is complicated, time-consuming and laborious, and the cost is high, so that the compound information obtained at one time is less, which cannot be effective. Complete high-throughput detection of unexpected effects of transgenes

Method used

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  • Method for obtaining metabolic difference between genetically modified and non-genetically modified maize based on UHPLC-MS (Ultra High Performance Liquid Chromatography-Mass Spectrometer)
  • Method for obtaining metabolic difference between genetically modified and non-genetically modified maize based on UHPLC-MS (Ultra High Performance Liquid Chromatography-Mass Spectrometer)
  • Method for obtaining metabolic difference between genetically modified and non-genetically modified maize based on UHPLC-MS (Ultra High Performance Liquid Chromatography-Mass Spectrometer)

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Embodiment 1

[0086] This example provides a UHPLC-MS-based method for obtaining metabolic differences between transgenic and non-transgenic maize, specifically:

[0087] 1) Preparation of samples to be tested:

[0088] 1) The young leaves of the transgenic MhSnf7 corn (TJ806) and TJ806 non-transgenic corn that grew to the 5-6 leaf stage were collected, quickly frozen in liquid nitrogen, and stored in a -80°C refrigerator for later use;

[0089] 2) Quickly place the leaves taken out of the cryogenic refrigerator into a liquid nitrogen pre-cooled mortar; pour a little liquid nitrogen, and grind with a pre-cooled pestle until the leaves are in a powder state;

[0090] 3) Weigh 20 ± 1 mg of crushed sample, place it in a 2 mL centrifuge tube, place the centrifuge tube containing the sample on ice; add two grinding beads, one large and one small, into the sample centrifuge tube. The diameter of the large magnetic bead is 5mm, and the diameter of the small magnetic bead is 3mm;

[0091] 4) Add ...

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Abstract

The invention relates to a method for obtaining the metabolic difference between genetically modified and non-genetically modified maize based on UHPLC-MS (Ultra High Performance Liquid Chromatography-Mass Spectrometer). According to the method, the extract of maize leaves is analyzed by adopting an ultra high performance liquid chromatography-high resolution mass spectrometry analysis platform toobtain metabolic profiles of the maize leaves, and then the data of the genetically modified and non-genetically modified maize is differentiated according to a multivariate statistical method to obtain compounds having important contribution to the phenotypic difference in metabolism. The method is simple to operate, capable of carrying out high throughput detection and high in detection resolution in particular, can eliminate interference, realizes efficient and reliable metabolite information identification and is more authentic and reliable in obtained result. The method can provide a basis for further study on the safety of the genetically modified maize.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for obtaining metabolic differences between transgenic and non-transgenic maize based on UHPLC-MS. Background technique [0002] Corn is the most widely distributed food crop in the world and one of the most important crops in China. However, biotic or abiotic stresses such as pests, diseases and drought seriously affect maize production. Obtaining transgenic corn with traits such as insect resistance, herbicide resistance, and disease resistance through transgenic methods and applying them in actual production can reduce the loss of corn yield, reduce the use of pesticides and fertilizers, and bring huge economic, social and environmental benefits. . At present, there are many genetically modified corn varieties that are resistant to insects and herbicides. With the large-scale commercial planting of genetically modified corn, people are concerned about its potenti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N30/88G01N2030/027G01N2030/062G01N2030/8809
Inventor 温洪涛张晓磊关海涛张瑞英李亮张爱红刘刚黄莹盈杜英秋陈国峰
Owner 黑龙江省农业科学院农产品质量安全研究所
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