Method for real-time monitoring experiment of living cells based on terahertz time-domain attenuated total reflection spectrums

A terahertz time domain, attenuated total reflection technology, applied in terahertz spectroscopy technology and application fields, to achieve the effects of reducing the influence of environmental factors, low absorption, and high refractive index

Pending Publication Date: 2017-12-01
INST OF FLUID PHYSICS CHINA ACAD OF ENG PHYSICS
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Problems solved by technology

[0004] The purpose of the present invention is based on the physical characteristics of terahertz light, to propose an analysis and measurement device based on terahertz time-domai...

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  • Method for real-time monitoring experiment of living cells based on terahertz time-domain attenuated total reflection spectrums
  • Method for real-time monitoring experiment of living cells based on terahertz time-domain attenuated total reflection spectrums

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Embodiment Construction

[0037] All features disclosed in this specification, or steps in all methods or processes disclosed, may be combined in any manner, except for mutually exclusive features and / or steps.

[0038] Such as figure 1As shown, in this scheme, the terahertz transmitting head generates picosecond terahertz pulse radiation through the polarization-maintaining fiber pigtail and the cable under the pumping and bias source loading of the femtosecond laser pulse; the terahertz probe passes through the polarization-maintaining fiber Pigtails and cables are pumped by external femtosecond laser pulses, combined with lock-in amplifier detection and fiber delay line scanning, to realize the time-domain electric field waveform detection of picosecond terahertz pulses; among them, femtosecond laser and bias source , lock-in amplifier and fiber delay line are all integrated in the mainframe of the all-fiber terahertz time-domain spectroscopy system. Terahertz module.

[0039] The 90° off-axis par...

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Abstract

The invention discloses a method and device for real-time monitoring experiment of living cells based on terahertz time-domain attenuated total reflection spectrums. The method and device are characterized in that by utilization of the characteristic of matching between the penetration depth of terahertz time-domain attenuated total reflection evanescent waves and the cell-layer thickness, terahertz time-domain attenuated total reflection measurement is carried out by using double ATR (Attenuated Total Reflection) prisms, the time-domain electric field and the complex dielectric constant (complex refraction index) of cells at the terahertz band can be directly obtained by a terahertz time-domain spectrum technology, so that real-time monitoring for the whole process of the internal physiological change of the cells can be realized, the limitation that the traditional transmission type or reflection type terahertz spectrums are easily influenced by high absorption rate of polar liquid is avoided, and the defect that the cells need to be dyed and invaded in the traditional cell-biology method is also avoided. The method and device disclosed by the invention have the advantages that the integration level of an optical system is high, the accuracy of a terahertz optical path is guaranteed, the adjustment for optical elements in the experiment is omitted, and convenience is brought for realizing carry and movement of the whole spectrum system, so that the method and device are very suitable for use in cell-biology laboratories.

Description

technical field [0001] The invention belongs to the field of terahertz spectroscopy technology and application, and specifically relates to a measurement and analysis device based on terahertz time-domain attenuated total reflection spectroscopy (THz TD-ATR spectroscopy). The invention is applicable to life processes such as physiology and pathology of living cells in vitro real-time monitoring. Background technique [0002] Cells are the basic unit of life structure and life activities. The growth and development of living organisms depends on cell proliferation, cell differentiation and cell apoptosis, and the pathogenesis of all diseases is also based on the study of cell pathology. Traditional cell biology methods include Western Blot, enzyme-linked immunohistochemistry (ELISA) and cell immunohistochemistry (IC), etc., which require complex sample pretreatment, such as cell fixation, component extraction, fluorescent labeling, etc. . These techniques have disadvantage...

Claims

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Application Information

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IPC IPC(8): G01N21/552G01N21/3586G01N21/01
CPCG01N21/01G01N21/3586G01N21/552G01N2021/3595G01N2201/063
Inventor 邹逸刘乔黄华川朱礼国李江翟召辉钟森城周平伟杜良辉王德田蒙建华彭其先李泽仁赵剑衡
Owner INST OF FLUID PHYSICS CHINA ACAD OF ENG PHYSICS
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