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Acremonium terricola culture and application thereof

The technology of Acremonium acremonium and its culture is applied in the culture of Acremonium acremonium and its application field, which can solve the problems that mRNA cannot be extended, mRNA cannot be matured, and there are no reports of visual fatigue drugs, etc., achieving low production cost and easy production. Effects obtained at scale

Active Publication Date: 2017-12-15
GUANGDONG RONGDA BIOENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Cordycepin has biological activities such as anti-tumor, anti-virus, anti-fungal, and immune regulation. The main mechanism of action is to replace adenosine and participate in cell metabolism, inhibit mRNA tailing, and cordycepin 5'triphosphate is connected to the 3' end of mRNA. Due to the lack of 3'-OH, the mRNA cannot be extended, and the mRNA cannot be matured, which ultimately inhibits the growth of tumor cells or pathogenic cells
[0005] At present, there is no report on the application of the Acremonium acremonium culture to prepare the medicine for relieving visual fatigue.

Method used

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  • Acremonium terricola culture and application thereof
  • Acremonium terricola culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 A kind of preparation method of Acremonium acremonium culture

[0031] The preparation method of described acremonium acremonium culture, comprises the steps:

[0032] S1. Acremonium acremonium was inoculated into the slant culture medium, and cultivated for 4 days at 25° C. to obtain the slant test tube strain; the slant medium used was potato 150 g / L, glucose 15 g / L, water 1000 mL, agar powder 20 g / L L, pH6;

[0033] S2. Shake flask culture with the slant test tube bacterial strain that step S1 makes, then move to seed tank and carry out seed culture, 25 ℃, 200rpm, cultivate 20h, obtain seed culture liquid; The seed culture medium used is: sucrose 30g / L , peptone 5g / L, yeast powder 5g / L, corn starch 10g / L, magnesium sulfate 0.1g / L, potassium dihydrogen phosphate 1g / L, pH 5;

[0034] S3. Inoculate the seed culture solution prepared in step S2 into a fermenter equipped with fermentation medium for fermentation according to the inoculum size of 20%. During...

Embodiment 2

[0037] Embodiment 2 A kind of preparation method of Acremonium acremonium culture

[0038] The preparation method of described acremonium acremonium culture, comprises the steps:

[0039] S1. Acremonium acremonium was inoculated into the slant culture medium, and cultivated for 5 days at 28° C. to obtain the slant test tube strain; the slant medium used was potato 200 g / L, glucose 25 g / L, water 1000 mL, agar powder 20 g / L L, the pH value is 7;

[0040] S2. Shake flask culture with the slant test tube strains prepared in step S1, then move to seed tank for seed culture, 35°C, 250rpm, cultivate for 50h, to obtain seed culture solution; used seed culture medium is: sucrose 50g / L , peptone 10g / L, yeast powder 10g / L, corn starch 25g / L, magnesium sulfate 1g / L, potassium dihydrogen phosphate 5g / L, pH value is 7;

[0041] S3. Inoculate the seed culture solution prepared in step S2 into a fermenter equipped with a fermentation medium for fermentation according to the inoculum size of...

Embodiment 3

[0044] Embodiment 3 A kind of preparation method of Acremonium acremonium culture

[0045] The preparation method of described acremonium acremonium culture, comprises the steps:

[0046] S1. Acremonium acremonium was inoculated into the slant medium, and cultivated for 5 days at 27°C to obtain the slant test tube strain; the slant medium used was potato 175g / L, glucose 20g / L, water 1000mL, agar powder 20g / L L, the pH value is 6.5;

[0047] S2. carry out shake flask culture with the slant test tube strain that step S1 makes, then move to seed tank and carry out seed culture, 30 ℃, 220rpm, cultivate 30h, obtain seed culture liquid; Used seed culture medium is: sucrose 45g / L , peptone 8g / L, yeast powder 8g / L, corn starch 15g / L, magnesium sulfate 0.7g / L, potassium dihydrogen phosphate 3.5g / L, pH 6.5;

[0048] S3. the seed culture solution that step S2 prepares is inoculated into the fermenter that fermentation medium is housed by inoculum amount 23% and fermented, and pH contro...

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Abstract

The invention belongs to the technical field of biological medicines and in particular relates to an acremonium terricola culture and application thereof. The acremonium terricola culture provided by the invention can effectively alleviate visual fatigue symptoms such as eye distension, sore eyes, photophobia, blurred vision, dry eyes and fading eyesight. The acremonium terricola culture provided by the invention is low in production cost, can be easily obtained in large scale and can be applied in preparation of medicines for relieving visual fatigue conveniently.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a culture of acremonium acremonium and its application. Background technique [0002] Visual fatigue is a common disease in ophthalmology at present. The symptoms of patients are various. The common ones are short-distance work cannot last long, pain in the eyes and around the eyes, blurred vision, dry eyes, tears, etc. In severe cases, headache, nausea, dizziness. It is not an independent disease, but a group of fatigue syndromes caused by various reasons. The reasons for its occurrence are also various, and the common ones are: (1) The reasons of the eye itself, such as myopia, hyperopia, astigmatism and other refractive errors, adjustment factors, eye muscle factors, conjunctivitis, keratitis, inappropriate glasses etc.; (2) Systemic factors, such as neurasthenia, physical fatigue, hysteria or menopausal women; (3) Environmental factors, such as insufficient ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/068A61P27/02A61P27/10C12R1/645
CPCA61K36/068C12N1/14
Inventor 蒋顺进方文棋黄炜乾郑雪媚唐谢芳张泳
Owner GUANGDONG RONGDA BIOENG CO LTD
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