A fluorescence sensitized detection method for ciprofloxacin by utilizing nitrogen-doped carbon quantum dots

A technology of carbon quantum dots and ciprofloxacin, which is applied in the field of nitrogen-doped carbon quantum dots for fluorescence sensitization detection of ciprofloxacin, can solve problems that have not yet been reported, and achieves high sensitivity, high fluorescence quantum yield, and method. simple effect

Active Publication Date: 2017-12-19
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, there have been many literature reports about nitrogen doped carbon quantum dots, and the fluorescence quantum yield of carbon quantum dots has been improved; the high sensitivity and high selectivity technical solutions provided by the present invention have not yet been reported

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  • A fluorescence sensitized detection method for ciprofloxacin by utilizing nitrogen-doped carbon quantum dots
  • A fluorescence sensitized detection method for ciprofloxacin by utilizing nitrogen-doped carbon quantum dots

Examples

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Embodiment 1

[0023] Embodiment 1: the determination of the content of ciprofloxacin in honey, the specific operation steps are as follows:

[0024] (1) Preparation of water-soluble nitrogen-doped fluorescent carbon quantum dots (CQDs): Weigh 2.0 g of soybean milk into 100 mL of ultrapure water, sonicate for 5 min to form a white suspension, and transfer it to a polytetrafluoroethylene reactor, Heating at 200°C for 12 hours, after natural cooling, first filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3000 Da for 24 hours to obtain water-soluble nitrogen-doped fluorescent carbon quantum dots;

[0025] (2) Drawing of the standard working curve: Take an appropriate amount of ciprofloxacin standard stock solution and add acetic acid-sodium acetate buffer solution with pH 5.0, and set the volume to 5 mL, and prepare the following concentrations of ciprofloxacin standard solution: 0.05, 0.1, 0.5,...

Embodiment 2

[0029] Embodiment 2: the content determination steps of ciprofloxacin in the chicken sample are:

[0030] (1) Preparation of water-soluble nitrogen-doped fluorescent carbon quantum dots: Weigh 3.0g soybean milk into 100mL ultrapure water, ultrasonicate for 5min to form a white suspension, transfer to a polytetrafluoroethylene reactor, and heat at 220°C 13h, after natural cooling, filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3200Da for 24 hours to obtain water-soluble nitrogen-doped fluorescent carbon quantum dots;

[0031] (2) drawing of standard working curve: with embodiment 1 step (2);

[0032](3) Chicken sample preparation: Weigh 4.00 g of chicken and put it in a 50 mL centrifuge tube, add 6 mL of 0.1 M phosphate buffer solution with pH=7, homogenize for 2 min, centrifuge at 5000 r / min for 8 min, and separate the supernatant in In another test tube, wash the blade of t...

Embodiment 3

[0034] Embodiment 3: the content determination steps of ciprofloxacin in the milk sample are:

[0035] (1) Preparation of water-soluble nitrogen-doped fluorescent carbon quantum dots: Weigh 4.5g of soybean milk into 100mL of ultrapure water, sonicate for 5 minutes to form a white suspension, transfer it to a polytetrafluoroethylene reactor, and heat at 210°C After natural cooling for 15 hours, first filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3500 Da for 24 hours to obtain water-soluble nitrogen-doped fluorescent carbon quantum dots;

[0036] (2) drawing of standard working curve: with embodiment 1 step (2);

[0037] (3) Determination of milk samples: Take 5 mL of milk, add 10 mL of methanol-water-formic acid (28:72:0.1, v / v) extract, 2 mL of 4% trichloroacetic acid in acetonitrile solution, vortex for 3 min , sonicate for 5 min, centrifuge at 10000r / min for 10 min, take ...

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Abstract

A fluorescence sensitized detection method for ciprofloxacin by utilizing nitrogen-doped carbon quantum dots is disclosed. Soymilk is adopted as a source of carbon and nitrogen, and the nitrogen-doped fluorescence carbon quantum dots are prepared through a one-step hydrothermal process in a high fluorescence quantum yield. The carbon quantum dots can enhance fluorescence of the ciprofloxacin, enhanced fluorescence signal intensity is in a certain linear relation with the ciprofloxacin concentration, and fluorospectrophotometry for detecting the ciprofloxacin is established according to the relation. The prepared carbon quantum dots have significant fluorescence sensibilization effects on the ciprofloxacin, are high in selectivity, and have characteristics of high sensitivity and specificity when the carbon quantum dots are used for fluorimetric determination of the ciprofloxacin.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for fluorescence-sensitized detection of ciprofloxacin by nitrogen-doped carbon quantum dots. Background technique [0002] Ciprofloxacin, also known as ciprofloxacin, is a chemically synthesized fluoroquinolone antibacterial drug. The drug has a wide antibacterial spectrum, strong bactericidal effect, and low toxicity. It has a strong and stable killing effect on Gram-negative bacteria and mycoplasma, and has a good antibacterial effect on Gram-positive bacteria, chlamydia and other pathogenic bacteria. It is used in the treatment of livestock and poultry chronic respiratory diseases, mycoplasma disease, colibacillosis, salmonellosis, pasteurellosis, etc. Ciprofloxacin is very popular for its rapid absorption by oral administration and injection, wide distribution in the body, high bioavailability, low toxicity, and no cross-resistance. The com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 杨亚玲胡枫杨德志
Owner KUNMING UNIV OF SCI & TECH
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