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A kind of extraction method of tortoise shell collagen polypeptide

A technology of collagen polypeptide and extraction method, which is applied in the field of tortoise shell collagen polypeptide extraction, can solve the problems of disintegration of the triple helix structure of collagen polypeptide, high requirements for equipment materials, and bitter smell of the product, so as to achieve rich polypeptide content and increase the dissolution rate , the effect of improving quality and taste

Active Publication Date: 2021-03-12
GUANGDONG SHENG HE TANG HEALTH FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are four main methods for preparing collagen peptides: acid method, alkali method, pyrolysis method and enzymatic method. Among them, the acid method extraction can better maintain the structural integrity of collagen peptides, but the quality is not high; the alkali method extraction is easy to cause The triple helix structure of collagen peptides disintegrates and requires high equipment materials; although the pyrolysis method is simple to operate, it takes a long time to hydrolyze and often needs to be operated under pressure; the cost of enzymatic hydrolysis is relatively high, and the product has a large bitter smell. At the same time, the color is dark yellow and the solubility is not good

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  • A kind of extraction method of tortoise shell collagen polypeptide

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Embodiment 1

[0028] A method for extracting tortoise shell collagen polypeptides, comprising the following steps:

[0029] (1) Pretreatment: Clean the tortoise shells, cook and degrease them at a temperature of 121°C and a pressure of 0.2MPa for 40 minutes, then dry them in a drying oven at a temperature of 70°C for 1 hour, and then pass them through a pulverizer for 100 minutes. Mesh sieve, obtain tortoise shell powder;

[0030] (2) Fermentation: Add water to the tortoise shell powder prepared in step (1) in the ratio of 1:25 to make tortoise shell powder solution, then add 2.0% glucose to adjust the pH of the solution to 6.0, and then inoculate 2.5% composite bacteria (Bacillus subtilis: Lactobacillus bulgaricus = 1:1), cultured on a shaking table at 40°C and 140r / min for 48h; after completion, sterilized at 121°C for 30min to obtain a fermentation broth;

[0031] (3) centrifugation: centrifuge the fermented liquid prepared in step (2) for 10 min at a rotating speed of 5000 r / min, and t...

Embodiment 2

[0034] A method for extracting tortoise shell collagen polypeptides, comprising the following steps:

[0035] (1) Pretreatment: Clean the tortoise shells, cook and degrease them at a temperature of 121°C and a pressure of 0.2MPa for 40 minutes, then dry them in a drying oven at a temperature of 70°C for 1 hour, and then pass them through a pulverizer for 100 minutes. Mesh sieve, obtain tortoise shell powder;

[0036] (2) Fermentation: adding water to the tortoise shell powder obtained in step (1) in a ratio of 1:28 to make tortoise shell powder solution, then adding 2.5% glucose, adjusting the pH of the solution to be 6.5, and then inoculating 3.0% compound bacteria (Bacillus subtilis: Lactobacillus bulgaricus = 1:1), cultured on a shaking table at 40°C and 140r / min for 48h; after completion, sterilized at 121°C for 30min to obtain a fermentation broth;

[0037] (3) centrifugation: centrifuge the fermented liquid prepared in step (2) for 10 min at a rotating speed of 5000 r / m...

Embodiment 3

[0040] A method for extracting tortoise shell collagen polypeptides, comprising the following steps:

[0041] (1) Pretreatment: Clean the tortoise shells, cook and degrease them at a temperature of 121°C and a pressure of 0.2MPa for 40 minutes, then dry them in a drying oven at a temperature of 70°C for 1 hour, and then pass them through a pulverizer for 100 minutes. Mesh sieve, obtain tortoise shell powder;

[0042] (2) Fermentation: Add water to the tortoise shell powder obtained in step (1) in a ratio of 1:30 to make tortoise shell powder solution, then add 3.0% glucose, adjust the pH of the solution to be 7.0, and then inoculate 3.5% composite bacteria (Bacillus subtilis: Lactobacillus bulgaricus = 1:1), cultured on a shaking table at 40°C and 140r / min for 48h; after completion, sterilized at 121°C for 30min to obtain a fermentation broth;

[0043] (3) centrifugation: centrifuge the fermented liquid prepared in step (2) for 10 min at a rotating speed of 5000 r / min, and ta...

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Abstract

The invention provides a method for extracting tortoise shell collagen polypeptide, comprising the following steps: (1) cleaning the tortoise shell, performing cooking and degreasing treatment, then drying, pulverizing, and passing through a 100-mesh sieve to obtain tortoise shell powder; (2) according to the quality of the feed liquid Add water to the tortoise shell powder at a ratio of 1:25-1:30 to make a tortoise shell powder substrate solution, add 2.0%-3.0% glucose at the same time, adjust the pH of the solution to 6.0-7.0, and then inoculate 2.5%-3.5 % of the composite bacteria are fermented and cultured, and then sterilized to obtain a fermented liquid; (3) the fermented liquid is subjected to centrifugation; (4) the supernatant is vacuum freeze-dried to obtain a tortoise shell collagen polypeptide product; the present invention The microbial fermentation method is used to save costs, which not only increases the yield of tortoise shell collagen polypeptide, but also has a special fragrance, very low bitter peptide content, rich polypeptide content, high solubility, and is easy to be digested by the human body.

Description

technical field [0001] The invention belongs to the technical field of biological separation and purification, and in particular relates to a method for extracting tortoise shell collagen polypeptide. Background technique [0002] Tortoise shell is the carapace and plastron of turtles. It is rich in tortoise shell glue (collagen), amino acids, fatty acids, minerals, polyphenols and steroids and other effective active ingredients. The effect of strengthening the kidneys, nourishing the heart and nourishing the blood is listed as the top grade in "Shen Nong's Materia Medica", and it is often used to treat symptoms such as yin deficiency hot flashes, bone steaming night sweats, dizziness, muscle flaccidity, weak heart and forgetfulness. [0003] Tortoise shell jelly is made from tortoise shells. It is a well-known nourishing medicine at home and abroad. It has excellent effects of nourishing yin, stopping bleeding and nourishing blood. The tortoise shell collagen polypeptide i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C12R1/125C12R1/225
CPCC12P21/00
Inventor 陆伟
Owner GUANGDONG SHENG HE TANG HEALTH FOOD CO LTD