A method for simultaneously detecting the contents of three alkaloids in Lycoris plants
A technology of Lycoris plants and alkaloids, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large consumption of plant samples and reagents, long extraction time, cumbersome operation, etc., and achieve saving of plant materials and extraction reagents, To ensure the accuracy of qualitative and quantitative, fast and simple results of the extraction process
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Embodiment 1
[0067] Embodiment 1 A method for simultaneously detecting the content of galantamine, ricolamin and lycorine in Lycoris genus Lycoris
[0068] Including the following steps:
[0069] S1. Preparation of standard solution:
[0070] Accurately weigh an appropriate amount of galantamine standard substance 0.010g (Gal, Galanthamine, C 17 h 21 NO 3 , molecular weight 287.359), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of Lycoramine standard substance 0.010g (Lycm, Lycoramine, C 17 h 23 NO 3 , molecular weight 289.375), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of lycorine standard substance 0.010g (Lyc, Lycorine, C 16 h 17 NO 4 , molecular weight 287.315), dissolved with methanol and fixed to 100ml to obtain its standard stock ...
Embodiment 2
[0114] Example 2 A method for simultaneously detecting the contents of galantamine, ricolamine and lycorine in Amaryllis genus Amaryllis
[0115] Including the following steps:
[0116] S1. Preparation of standard solution:
[0117] Accurately weigh an appropriate amount of galantamine standard substance 0.010g (Gal, Galanthamine, C 17 h 21 NO 3 , molecular weight 287.359), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of Lycoramine standard substance 0.010g (Lycm, Lycoramine, C 17 h 23 NO 3 , molecular weight 289.375), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of lycorine standard substance 0.010g (Lyc, Lycorine, C 16 h 17 NO 4 , molecular weight 287.315), dissolved with methanol and fixed to 100ml to obtain its standard sto...
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