A method for simultaneously detecting the contents of three alkaloids in Lycoris plants

A technology of Lycoris plants and alkaloids, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large consumption of plant samples and reagents, long extraction time, cumbersome operation, etc., and achieve saving of plant materials and extraction reagents, To ensure the accuracy of qualitative and quantitative, fast and simple results of the extraction process

Active Publication Date: 2020-02-14
SHANGHAI ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Li Mingkai (Establishment of Lycoris alkaloid analysis method and its biosynthesis research, Anhui Agricultural University, 2012: 1-69) realized the preliminary separation of alkaloids by using thin-layer chromatography separation technology, and found that two-way thin-layer chromatography Compared with one-way chromatography, it is more suitable for the separation of many different alkaloid substances. This method mainly realizes the Preliminary characterization of alkaloid species in a sample, but not for accurate structural and quantitative analysis
[0005] Quan et al. (Quan et al., Photosynthetic characteristics of Lycoris aurea and monthly dynamics of alkaloid contents in its bulbs, African Journal of Biotechnology, 11(15): 3686-3691, 2012) utilized high-performance liquid chromatography detection technology, through samples and known The comparison of the retention time and the peak area of ​​the alkaloid standard substance under the same conditions carried out the quantitative research of galantamine and lycorine in Lycoris hudixiao, and its single extraction of Lycoris bulb material needs fresh sample 20g ( water content of about 90%), equivalent to 2g of Lycoris bulb dry sample, need to consume 110ml of methanol, 10ml of hydrochloric acid, 15ml of chloroform, which consumes a lot of plant samples and reagents, and at the same time, it only adopts the method of HPLC, according to the retention time of the substance. Content detection cannot be accurately qualitative for the substance to be tested, so the quantitative result of the substance is unreliable, and the structure, qualitative and precise quantitative analysis cannot be carried out
[0006] Sahar et al. (Sahar et al., 4'-O-Methylnorbelladine feeding enhances galanthamine and lycorine production by Leucojum aestivum L.shoot cultures, Engineering in Life Sciences, 00: 1-6, 2015) adopted the method of HPLC-ESI-MS, for Quantitative analysis of lycorine and galantamine in the lotus buds of Xia Xuepian, the elution time of lycorine is 25.5min, the elution time of galantamine is 32.2min, the HPLC analysis time is relatively long, about 36 minutes, the content detection is carried out according to the retention time and precursor ion information. Compared with the method of Quan et al., the accuracy of this method is improved, but the obvious defect is that there may be many kinds of material components with the same elution time and precursor ion size , so there are still defects in qualitative and quantitative accuracy
[0007] Moreover, in the prior art, there are also problems of long extraction time and cumbersome operation. For example, in the method of Quan et al., the main extraction steps are drying, filtration, methanol extraction for 3 hours, filtration, evaporation, pH adjustment, and chloroform extraction for 3 times. , evaporation, methanol reconstitution, filtration
In the method of Sahar et al. (Sahar et al., 2015), the main extraction steps are: methanol extraction for 24 hours, concentration, sulfuric acid acidification, chloroform extraction, evaporation, reconstitution, and filtration. These cumbersome steps are all alkaloid substances. Quick Quantitative Analysis Brings Inconvenience

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for simultaneously detecting the contents of three alkaloids in Lycoris plants
  • A method for simultaneously detecting the contents of three alkaloids in Lycoris plants
  • A method for simultaneously detecting the contents of three alkaloids in Lycoris plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1 A method for simultaneously detecting the content of galantamine, ricolamin and lycorine in Lycoris genus Lycoris

[0068] Including the following steps:

[0069] S1. Preparation of standard solution:

[0070] Accurately weigh an appropriate amount of galantamine standard substance 0.010g (Gal, Galanthamine, C 17 h 21 NO 3 , molecular weight 287.359), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of Lycoramine standard substance 0.010g (Lycm, Lycoramine, C 17 h 23 NO 3 , molecular weight 289.375), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of lycorine standard substance 0.010g (Lyc, Lycorine, C 16 h 17 NO 4 , molecular weight 287.315), dissolved with methanol and fixed to 100ml to obtain its standard stock ...

Embodiment 2

[0114] Example 2 A method for simultaneously detecting the contents of galantamine, ricolamine and lycorine in Amaryllis genus Amaryllis

[0115] Including the following steps:

[0116] S1. Preparation of standard solution:

[0117] Accurately weigh an appropriate amount of galantamine standard substance 0.010g (Gal, Galanthamine, C 17 h 21 NO 3 , molecular weight 287.359), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of Lycoramine standard substance 0.010g (Lycm, Lycoramine, C 17 h 23 NO 3 , molecular weight 289.375), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the stock solution concentration is 100 μg / mL; accurately weigh an appropriate amount of lycorine standard substance 0.010g (Lyc, Lycorine, C 16 h 17 NO 4 , molecular weight 287.315), dissolved with methanol and fixed to 100ml to obtain its standard sto...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for simultaneously detecting the contents of three alkaloids in a lycoris plant, which is capable of quickly extracting and qualitatively and quantitatively analyzingalkaloid matters of galanthamine, lycoramine and lycorine in the lycoris plant. According to the method disclosed by the invention, gradient eluting is carried out by taking an LC (Liquid Chromatography)-MS (Mass Spectrometry) technology as the basis, analysis is carried out in an MRM (Multiple Reaction Monitoring) mode under positive ion scanning by connecting LC with tandem MS, the three alkaloid matters in the lycoris plant can be quickly extracted and accurately, qualitatively and quantitatively analyzed, the analysis time can be shortened to a large extent, the analysis accuracy is increased, a detection result can be obtained within a program time which is not more than 6.0 minutes, the retention time of the three alkaloids is within 3 minutes, the adding standard recovery rate is 97.2 to 100.2 percent, the RSD (Relative Standard Deviation) is 0.8 to 4.3 percent, and the analysis speed and the analysis accuracy are greatly increased; an effective and accurate analysis method is provided for researching an accumulation law and constructive metabolism regulation of the three alkaloids in the lycoris plant.

Description

technical field [0001] The invention belongs to the technical field of detection of alkaloid substances, and in particular relates to a method for simultaneously detecting the contents of three alkaloids in Lycoris plants. Background technique [0002] Lycoris spp. is a bulbous herb of Amaryllidaceae (Amarylllidaceae), many of which are unique ornamental and medicinal flowers in China, rich in a variety of unique alkaloid components of Lycoris spp. So far, more than 500 alkaloid compounds with different structures have been isolated from Lycoris plants. Phytochemical studies have shown that these compounds have various pharmacological functions and have high medicinal value. Among them, galantamine and ricolamine can be used to treat Alzheimer's disease (Alzheimer's disease, early symptoms of Alzheimer's disease), and lycorine has antibacterial, antimalarial, tumor treatment, antiviral and cardiovascular protection effects. important value. Up to now, wild Lycoris resource...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/14G01N30/34
Inventor 蔡友铭张永春李青竹杨柳燕李心
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products