Kit for detecting free semen elastinase and application thereof
An elastase and detection kit technology, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of unsuitable popularization and application, complicated operation steps, and many sample adding procedures, and achieves short detection time, Accurate and repeatable test results
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Embodiment 1
[0038] Semen free elastase detection kit, including the following components:
[0039] R1 reagent: dimethyl sulfoxide dissolved in Hepes buffer solution, 0.75g polyoxyethylene lauryl ether, 0.3mol sodium chloride and 0.03mol N-(2-hydroxyethyl)piperazine-N' per liter of Hepes buffer solution - 2-ethanesulfonic acid, adjust its pH to 8.0 to obtain a Hepes buffer solution, add 19 ml of dimethyl sulfoxide to this buffer solution (1 L) to obtain R1 reagent.
[0040] R2 reagent: After completely dissolving N-methoxysuccinyl-propionamide-propionylamide-prolylamide-valine-4-nitroanilide in dimethyl sulfoxide, add Hepes buffer solution ( Same as in R1) dissolved to obtain R2 reagent, containing 3.7mmol N-methoxysuccinyl-propionylamide-propionylamide-prolineamide-valine-4-nitroanilide in every liter of R2 reagent, 19ml of dimethylsulfoxide and 0.0245mol of N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid.
[0041] The calibrator is an elastase solution, wherein the elastase activ...
Embodiment 2
[0045] Semen free elastase detection kit, including the following components:
[0046] R1 reagent: dimethyl sulfoxide dissolved in Hepes buffer solution, containing 1.2g polyoxyethylene lauryl ether, 0.1mol sodium chloride and 0.05mol N-(2-hydroxyethyl)piperazine-N per liter of Hepes buffer solution '-2-ethanesulfonic acid, adjust its pH=9.0 to obtain Hepes buffer solution, add 30ml dimethyl sulfoxide to this buffer solution (1L) to obtain R1 reagent.
[0047] R2 reagent: After completely dissolving N-methoxysuccinyl-propionamide-propionylamide-prolylamide-valine-4-nitroanilide in dimethyl sulfoxide, add Hepes buffer solution ( Same as in R1) dissolved to obtain R2 reagent, containing 5.0mmol N-methoxysuccinyl-propionylamide-propionylamide-proylamide-valine-4-nitroanilide in every liter of R2 reagent, 30ml dimethyl sulfoxide, 0.0485mol N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid.
[0048] The calibrator is an elastase solution, wherein the elastase activity is 10 U...
Embodiment 3
[0052] Semen free elastase detection kit, including the following components:
[0053] R1 reagent: dimethyl sulfoxide dissolved in Hepes buffer solution, containing 0.3g polyoxyethylene lauryl ether, 0.5mol sodium chloride and 0.01mol N-(2-hydroxyethyl)piperazine-N per liter of Hepes buffer solution '-2-ethanesulfonic acid, adjust its pH=7.0 to obtain Hepes buffer solution, add 8ml dimethyl sulfoxide to this buffer solution (1L) to obtain R1 reagent.
[0054] R2 reagent: After completely dissolving N-methoxysuccinyl-propionamide-propionylamide-prolylamide-valine-4-nitroanilide in dimethyl sulfoxide, add Hepes buffer solution ( Same as in R1) dissolving to obtain R2 reagent, containing 2.4mmol N-methoxysuccinyl-propionylamide-propionylamide-prolineamide-valine-4-nitroanilide in every liter of R2 reagent, 8ml dimethyl sulfoxide, 0.0097mol N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid.
[0055] The calibrator is elastase solution, wherein the activity of elastase is 5U / ...
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