Kit for detecting free semen elastinase and application thereof
An elastase and detection kit technology, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of unsuitable popularization and application, complicated operation steps, and many sample adding procedures, and achieves short detection time, Accurate and repeatable test results
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0037] Example 1
[0038] Semen free elastase detection kit, including the following components:
[0039] R1 reagent: dimethyl sulfoxide dissolved in Hepes buffer solution, 0.75g polyoxyethylene lauryl ether, 0.3mol sodium chloride and 0.03mol N-(2-hydroxyethyl)piperazine-N' per liter of Hepes buffer solution -2-ethanesulfonic acid, adjust its pH=8.0 to obtain Hepes buffer solution, add 19ml dimethyl sulfoxide to this buffer solution (1L) to obtain R1 reagent.
[0040] R2 reagent: After completely dissolving N-methoxysuccinyl-propionylamino-propionylamino-proylamide-valine-4-nitroanilide in dimethyl sulfoxide, add Hepes buffer solution ( Same as in R1) Dissolve to get R2 reagent. Each liter of R2 reagent contains 3.7mmol N-methoxysuccinyl-propionylamino-propionylamino-proylamine-valine-4-nitroanilide, 19ml dimethyl sulfoxide and 0.0245mol N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid.
[0041] The calibrator is an elastase solution, in which the elastase activity is 7.5 U / L,...
Example Embodiment
[0044] Example 2
[0045] Semen free elastase detection kit, including the following components:
[0046] R1 reagent: dimethyl sulfoxide dissolved in Hepes buffer solution, each liter of Hepes buffer solution contains 1.2g polyoxyethylene lauryl ether, 0.1mol sodium chloride and 0.05mol N-(2-hydroxyethyl)piperazine-N '-2-ethanesulfonic acid, adjust its pH=9.0 to obtain Hepes buffer solution, add 30ml dimethyl sulfoxide to this buffer solution (1L) to obtain R1 reagent.
[0047] R2 reagent: After completely dissolving N-methoxysuccinyl-propionylamino-propionylamino-proylamide-valine-4-nitroanilide in dimethyl sulfoxide, add Hepes buffer solution ( Same as in R1) Dissolve to get R2 reagent, each liter of R2 reagent contains 5.0mmol N-methoxysuccinyl-propionylamino-propionylamino-proylamine-valine-4-nitroanilide, 30ml dimethyl sulfoxide, 0.0485mol N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid.
[0048] The calibrator is an elastase solution, in which the elastase activity is 10...
Example Embodiment
[0051] Example 3
[0052] Semen free elastase detection kit, including the following components:
[0053] R1 reagent: dimethyl sulfoxide dissolved in Hepes buffer solution, each liter of Hepes buffer solution contains 0.3g polyoxyethylene lauryl ether, 0.5mol sodium chloride and 0.01mol N-(2-hydroxyethyl)piperazine-N '-2-ethanesulfonic acid, adjust its pH=7.0 to obtain Hepes buffer solution, add 8ml dimethyl sulfoxide to this buffer solution (1L) to obtain R1 reagent.
[0054] R2 reagent: After completely dissolving N-methoxysuccinyl-propionylamino-propionylamino-proylamide-valine-4-nitroanilide in dimethyl sulfoxide, add Hepes buffer solution ( Same as in R1) Dissolve to get R2 reagent. Each liter of R2 reagent contains 2.4mmol N-methoxysuccinyl-propionylamino-propionylamino-proylline-valine-4-nitroanilide, 8ml dimethyl sulfoxide, 0.0097mol N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid.
[0055] The calibrator is an elastase solution, in which the elastase activity is 5U / L,...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap