Method for storing Rhinopithecus roxellana feces sample for DNA extraction

A preservation method, the technology of golden monkey, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problem that there is no general method for the preservation of feces samples, and achieve the effect of efficient DNA extraction and effective preservation

Inactive Publication Date: 2018-01-16
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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Problems solved by technology

Due to the environmental conditions of different research objects, the food characteristics of the research objects, individual differences, etc., there are still speci...

Method used

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Embodiment Construction

[0011] (1) Collection and storage methods of stool samples

[0012] The feces were collected in Shennongjia National Nature Reserve, Hubei, and the samples were preserved by drying and freezing. The dry-refrigerated storage method is to collect the collected samples in a 15ml collection tube, which contains about 2 / 3 of silica gel, lay sterile filter paper on the surface, and store it in a -20°C refrigerator.

[0013] (2) Extraction of DNA from stool samples

[0014] Fecal DNA extraction is performed using the QIAamp DNA Stool Kit (QIAGEN) kit. The specific steps and methods of DNA extraction are adjusted according to the actual situation. The specific operations are as follows:

[0015] 1) Take about 0.3g of feces surface sample into a new 2 ml centrifuge tube;

[0016] 2) Put 1.6ml of ASL lysis buffer into the centrifuge tube containing the feces sample, shake evenly, make the sample fully contact with the lysate, let it stand for more than 3 hours, and shake once in abo...

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Abstract

The invention relates to a method for storing a Rhinopithecus roxellana feces sample for DNA extraction. The method specifically comprises the following steps: (1) the feces sample is placed in a 15mlcollection tube, silica gel is contained in the collection tube, and sterile filter paper is spread on the surface of the silica gel; (2) the collection tube is stored in a refrigerator at the temperature of subzero 20 DEG C. The feces sample can be stored simply, accurately and effectively with the method, and guarantee is provided for research in genetic diversity, conservation genetics and thelike for Rhinopithecus roxellana.

Description

technical field [0001] The invention relates to a method for preserving feces samples of Sichuan snub-nosed monkeys for DNA extraction, in particular to a method for preserving feces samples based on genetic diversity research of Sichuan snub-nosed monkeys. Background technique [0002] The use of fecal samples to extract animal DNA has become a common non-invasive sampling method in wildlife conservation research. This method is very useful for studying large, rare and hard-to-see endangered animals (Smith et al. , 2000; Li Ming et al., 2001), and have solved problems in molecular ecology and population genetics such as individual identification, genetic diversity, genetic structure, sex ratio structure, kinship, and phylogenetic evolution of rare and endangered wild animals, and Combined with classical ecological methods to solve the problems related to the protection of endangered species such as species population survey, monitoring, and protection construction (Zhang Yu...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 张于光周芸芸
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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