Liver cancer marker lncRNA ENST00000620463.1 and application thereof

A technology of DNA molecules and uses, applied in the field of biomedicine, can solve the problem that the molecular biology mechanism of liver cancer has not yet been clearly studied.

Active Publication Date: 2018-01-16
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular biological mechanism of the occurrence, ...

Method used

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  • Liver cancer marker lncRNA ENST00000620463.1 and application thereof
  • Liver cancer marker lncRNA ENST00000620463.1 and application thereof
  • Liver cancer marker lncRNA ENST00000620463.1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1, screening of liver cancer markers

[0074] Next-generation sequencing analysis was performed on the cancer tissues and paired paracancerous tissues of 20 patients with liver cancer (the patients all gave informed consent, and all the above-mentioned samples were obtained with the consent of the organization ethics committee), and a liver cancer marker was found, which was a kind of LncRNA, named It is ENST00000620463.1, as shown in sequence 1 of the sequence listing. The corresponding DNA of ENST00000620463.1 is shown in sequence 2 of the sequence listing. Compared with adjacent tissues, the expression level of ENST00000620463.1 in HCC tissues was significantly higher than that in adjacent tissues.

Embodiment 2

[0075] Example 2, qPCR sequencing to verify the differential expression of ENST00000620463.1

[0076] 1. The cancer tissues and paracancerous tissues of 102 liver cancer patients were collected. The patients all gave informed consent, and all the above specimens were obtained with the approval of the organizational ethics committee.

[0077] 2. Extract tissue RNA from each sample in step 1, and reverse transcribe it into cDNA.

[0078] 3. Using the cDNA of each sample obtained in step 2 as a template, perform qPCR to detect the expression of ENST00000620463.1 (using GAPDH as an internal reference gene). Primers F1 and R1 were used to detect the expression of ENST00000620463.1, and primers F2 and R2 were used to detect the expression of GAPDH gene.

[0079] F1: 5'-GGTGTAAGCGGTGGTTA-3' (sequence 3 of the sequence listing);

[0080] R1: 5'-CCAGTTCTGGGCTGGGATGT-3' (sequence 4 of the sequence listing);

[0081] F2: 5'-GGTGGTCTCCTCTGACTTCAACA-3';

[0082] R2: 5'-GTTGCTGTAGCCAAAT...

Embodiment 3

[0084] Example 3, ROC Curve Analysis of ENST00000620463.1 in Patient Cancer Tissue and Paracancer Tissue

[0085] The receiver operating characteristics of ENST00000620463.1 were analyzed using the pROC package in R language, the binomial exact confidence space was calculated, and the ROC curve was drawn.

[0086] The result is as figure 2 shown. The AUC value of ENST00000620463.1 is 0.85, and has good specificity and sensitivity, indicating that ENST00000620463.1 has high accuracy in the diagnosis of liver cancer.

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Abstract

The invention discloses a liver cancer marker lncRNA ENST00000620463.1 and application thereof. The invention provides a tumor marker which is LncRNA; the tumor marker is named as ENST00000620463.1; the ENST00000620463.1 is RNA expressed as a sequence 1 in a sequence table; DNA corresponding to ENST00000620463.1 is DNA expressed as a sequence 2 in the sequence table. The invention firstly discovers that the ENST00000620463.1 in the liver cancer is greatly increased; an experiment proves that shRNA interference silencing ENST00000620463.1 is capable of effectively inhibiting proliferation, migration and invasion of the liver cancer cells; a novel method is provided for the individualized treatment of the liver cancer.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to a liver cancer marker lncRNA ENST00000620463.1 and its application. Background technique [0002] Primary hepatocellular carcinoma (hepatocellular carcinoma, HCC) (hereinafter referred to as liver cancer) is a common malignant tumor in my country, with the second highest mortality rate among cancers, with an incidence of about 350,000 people every year. The morbidity and mortality of liver cancer have always been high. Because of its high malignancy and rapid disease progression, most patients are already in the middle or late stage when clinically discovered, with high mortality and recurrence rates and poor prognosis. The current conventional treatments mainly include surgical resection, liver transplantation, local ablation, transarterial chemoembolization, radiotherapy, molecular targeted therapy, comprehensive therapy, etc. Unsatisfactory. [0003] The occurrence and dev...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/6886A61K45/00A61P35/00
Inventor 张绍庚杨鹏辉程思杰王兆海张培瑞余灵祥洪智贤
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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