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A lead-adsorbing and heavy metal-tolerant bacterium and its application

A heavy metal and tolerance technology, applied in the field of environmental biology, can solve the problems of high cost, incomplete treatment, secondary pollution, etc., and achieve the effect of high application value, high efficiency and high tolerance

Inactive Publication Date: 2020-03-10
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional treatment methods include chemical precipitation, redox, ion exchange, membrane treatment, and electrochemical methods, but there are high costs, incomplete treatment, poor repair effect, and easy to cause secondary pollution.

Method used

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  • A lead-adsorbing and heavy metal-tolerant bacterium and its application
  • A lead-adsorbing and heavy metal-tolerant bacterium and its application
  • A lead-adsorbing and heavy metal-tolerant bacterium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] [Example 1] Screening and tolerance test of lead-tolerant bacteria

[0029] The experimental soil samples were taken from the rhizosphere and non-rhizosphere soil around a radio equipment factory in Wuhan City, Hubei Province. After the soil samples were collected, they were returned to the laboratory and immediately stored at -20°C to kill insects and plant tissues in the soil as well as some Virus. After 10 hours of treatment, it was thawed at 4°C, and the excess soil samples were stored in a 4°C refrigerator. Take 10g soil sample, add 100mL sterile water and shake for 2h, take the supernatant and adopt conventional gradient dilution plate coating culture method (medium containing Pb 2+ The concentration is about 100mg / L), cultured in a constant temperature incubator at 28°C for 24-48h, and the strains with good growth potential were selected to be purified and re-screened on the selection medium with a concentration of 200, 300, and 400mg / L in turn. The basal mediu...

Embodiment 2

[0033] [Example 2] identification of bacterial strains

[0034] Inoculate the purified strain on solid LB medium and culture for 2 days to observe the morphological characteristics of the colony of the strain on the solid plate: the colony is round, creamy yellow to brownish yellow, opaque, the diameter of the mature colony is about 0.6cm, the colony is moist, sticky and easy to pick Pick. After the purified strains were inoculated in liquid LB medium, samples were taken regularly within a certain period of time to determine the OD 600 , to draw the strain growth curve. The logarithmic growth phase will be reached within 12 hours in liquid LB culture medium shaker shaker constant temperature 37 ℃, and the stable phase will be reached within 24 hours. , mostly round or oval, with smooth edges, the color of mature colonies turns brownish yellow, and the colonies may become wrinkled near the edges; the physical and chemical properties of the strains are mainly identified by ref...

Embodiment 3

[0040] [Example 3] bacterial strain removes Pb 2+ Characteristic research

[0041] Experiments on the influence of pH, adsorption time, and initial concentration of heavy metal ions on the adsorption effect. The bacterial strain was expanded and cultivated in LB culture medium at 37°C and 180rpm for 12h, and the obtained activated strain was centrifuged at 8000rpm for 10min. 2.3, about 20g / L) for later use. Use 1mol / L NaOH and HNO3 to adjust the pH of a solution containing a certain amount of Pb2+ to be 3, 4, 5, 6, 7; Pb 2+ The initial concentration is 25, 50, 75, 100, 150, 200 mg / L; inoculate 5% (v / v) prepared concentrated bacterial solution in a certain concentration of Pb-containing 2+ In the middle, take the supernatant regularly to measure Pb 2+ content.

[0042] (1) pH value. Depend on image 3 The pH of the medium and low initial pH experimental group changes with time, and it can be known that the bacteria has an alkali-producing effect; combined with the change...

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Abstract

The invention discloses a bacterium capable of adsorbing lead and enduring heavy metals and application of the bacterium. The bacterium is classified and named as bacillus megatherium Z-y3, and the preservation number of the bacterium is CCTCC NO.M2017312. The strain can grow well in a 1000mg / L solid culture medium. The strain disclosed by the invention has an alkali generation capability and hasa relatively good lead removal property at different pH values, and the adsorption amount of a cultured bacterium can be maximum, namely about 20g / L, within a short time. The lead-removal metabolite of the strain is non-toxic and is environmentally friendly. Therefore, the strain has very high application values in treatment on lead heavy metal polluted water bodies, soil environment treatment andcomprehensive treatment on industrial field pesticide pollution.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a bacterium which has adsorption and removal effects on lead and is tolerant to some heavy metals. Background technique [0002] With the continuous development of industrial production, heavy metal pollution in water is becoming more and more serious. Due to the particularity of the chemical behavior of heavy metals, they cannot be decomposed, can accumulate through the food chain, cause chronic poisoning, and can also combine with other organic substances in the environment to form more toxic organic toxins, causing great harm to organisms and the environment. Traditional treatment methods include chemical precipitation, oxidation-reduction, ion exchange, membrane treatment, and electrochemical methods, but there are problems such as high cost, incomplete treatment, poor repair effect, and easy to cause secondary pollution. The bioremediation method estab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/11C02F101/20C02F101/22
Inventor 陈兰洲赵瑞雪武艳芳梁亚楠田江柯檀刘思瑶
Owner WUHAN UNIV
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