Method for preparing sulfydryl-rich keratin and promoting rapid gelating of silk fibroin and application

A technology for silk fibroin and keratin, which is applied to skin care preparations, pharmaceutical formulations, cosmetic preparations and other directions, can solve the problems of unfavorable silk fibroin biocompatibility, slow gelation speed and the like, and achieves a simple and convenient preparation method. , the effect of good applicability

Inactive Publication Date: 2018-01-30
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is: the gelation speed of pure silk fibroin solution is slow without cross-linking agent and external stimulation, and cross-linking agent is not conducive to the biocompatibility of silk fibroin

Method used

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  • Method for preparing sulfydryl-rich keratin and promoting rapid gelating of silk fibroin and application
  • Method for preparing sulfydryl-rich keratin and promoting rapid gelating of silk fibroin and application
  • Method for preparing sulfydryl-rich keratin and promoting rapid gelating of silk fibroin and application

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A kind of mercapto-rich keratin and its method for promoting rapid gelation of silk fibroin:

[0031] Weigh 5g of washed and shredded wool and add it into 50ml of a mixed solution containing 0.1M sodium sulfide, 8M urea and 0.2M sodium lauryl sulfate and stir at a speed of 60 rpm to dissolve 7 hours, after the wool reaction is completely filtered, dialyzed, concentrated and diluted to prepare a wool keratin aqueous solution with a concentration of 50 mg / mL carrying a large number of mercapto groups. Weigh 10 g of silkworm cocoons cut into pieces and put them into 2 L of 0.5% sodium bicarbonate solution, boil for 30 min and repeat twice, remove silk sericin and wash with deionized water 4 times. Weigh 5g of dried degummed silk and put it into 30mL lithium bromide solution with a concentration of 9.3M, place it at 50°C for 4 hours, take it out, dialyze it in a dialysis bag with a molecular weight of 3500D, dilute and filter to obtain Pure silk fibroin solution with a mas...

Embodiment 2

[0034] A kind of mercapto-rich keratin and its method for promoting rapid gelation of silk fibroin:

[0035] Weigh 4g of washed and shredded wool, add 60ml of mercaptoethanol with a concentration of 0.2M, urea with a concentration of 8M and sodium lauryl sulfate with a concentration of 0.3M and stir to dissolve at a speed of 50 rpm. Hours, after the wool reaction is completely filtered, dialyzed, concentrated and diluted, a wool keratin aqueous solution with a concentration of 40 mg / mL is prepared. Weigh 6g of dried degummed silk and put it into 30mL lithium bromide solution with a concentration of 9.3M, place it at 60°C and dissolve it for 6 hours, take it out, dialyze it with a dialysis bag with a molecular weight of 3500D, dilute and filter to obtain Pure silk fibroin solution with a mass concentration of 40mg / mL.

[0036] Measure 400 μL and 600 μL of the keratin solution and silk fibroin solution with a concentration of 50 mg / mL, respectively, with a pipette, mix well and...

Embodiment 3

[0038]The washed discarded human hair was stirred and dissolved with 4M sodium hydroxide and 0.2M mercaptoethanol, and after filtration, dialysis, concentration and dilution, an aqueous solution of human hair keratin with a concentration of 50 mg / mL was obtained. Weigh 10 g of silkworm cocoons cut into pieces and put them into 2 L of 0.5% sodium bicarbonate solution, boil for 30 min and repeat twice, remove silk sericin and wash with deionized water 4 times. Weigh 5g of dried degummed silk and put it into 30mL lithium bromide solution with a concentration of 9.3M, place it at 50°C for 4 hours, take it out, dialyze it in a dialysis bag with a molecular weight of 3500D, dilute and filter to obtain Pure silk fibroin solution with a mass concentration of 50mg / mL.

[0039] Measure 600 μL and 400 μL of keratin solution and silk fibroin solution with a concentration of 50 mg / mL, respectively, with a pipette, mix well and move them into a columnar mold. Let it stand for an hour, take...

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Abstract

The invention discloses a method for preparing sulfydryl-rich keratin and promoting rapid gelating of silk fibroin and application to the skin care products as well as repairing and reconstructing ofartificial skin, dressing or injured tissues. The preparation method comprises extracting a sulfydryl-rich keratin solution, extracting a natural silk fibroin solution, preparing a keratin/silk fibroin hydrogel and the like. The keratin carrying a large quantity of sulfydryls is used to promote the rapid gelating of the silk fibroin solution, the keratin and silk fibroin solutions are mixed and then promote a beta-microcrystal to form a three-dimensional net structure under the actions of the macromolecular chain of the amino acid and the hydrogen bond together, so that the formation of the hydrogel is accelerated, and the obtained hydrogel has an excellent mechanical property.

Description

technical field [0001] The present invention relates to a kind of keratin rich in sulfhydryl groups and its promotion of rapid gelation of silk fibroin, a preparation method and its application, in particular to a kind of keratin rich in sulfhydryl groups obtained by using a reducing agent to break the disulfide bonds in keratin macromolecules. The keratin solution is mixed with the silk fibroin solution to quickly form a gel, the preparation method and its application. Background technique [0002] Hydrogels formed by natural polymers have attracted the attention of scholars due to their high water content, good biocompatibility and rich sources, especially hydrogels with silk fibroin as the main component are considered to have potential. of biological materials. [0003] The β-sheet structure rich in silk fibroin itself can form a three-dimensional network structure hydrogel, but the gelation process is long, and usually needs to be regulated by physical methods such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00C08J3/075C08L89/00A61K8/65A61K8/64A61K8/04A61Q19/00A61L15/32A61L15/42A61L27/26A61L27/50A61L27/52A61L27/60
Inventor 涂凰于伟东刘向阳
Owner DONGHUA UNIV
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