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Application of ABI5 protein and coding gene in regulating oxidative stress resistance of plant seeds

A technology of oxidative stress and coding genes, applied in the application field of ABI5 protein and its coding genes in regulating the resistance of plant seeds to oxidative stress, to achieve the effect of promoting germination and large economic benefits

Active Publication Date: 2018-02-02
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these usual treatment methods and strategies are to use a variety of substances mixed according to a specific formula, and most of these methods have obvious effects on one or a few specific plant seeds, and there are obvious species limitations

Method used

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  • Application of ABI5 protein and coding gene in regulating oxidative stress resistance of plant seeds
  • Application of ABI5 protein and coding gene in regulating oxidative stress resistance of plant seeds
  • Application of ABI5 protein and coding gene in regulating oxidative stress resistance of plant seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the acquisition and identification of ABI5 transgenic plants

[0056] The ABI5 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, and sequence 1 consists of 2765 nucleotides, which is the The sequence of the ABI5 gene in the Arabidopsis genome, wherein the 2089-2169, 2242-2329 and 2360-2453 are intron sequences; the cDNA sequence of the ABI5 gene is shown in sequence 2 in the sequence table, Sequence 2 consists of 1720 nucleotides and is the cDNA sequence of the ABI5 gene, wherein the 176th-1504th position is the coding sequence (ORF); both sequence 1 and sequence 2 encode the protein shown in sequence 3 in the sequence listing, sequence 3 consists of 442 amino acid residues.

[0057] 1. Construction of recombinant expression vector pCAMBIA-1300-221-ABI5

[0058] The total RNA of Arabidopsis wild-type (Col-O ecotype) was extracted, and cDNA was obtai...

Embodiment 2

[0097] Example 2, ABI5 transgenic plants analysis test for tolerance to oxidative stress during seed germination

[0098] The plants to be tested are: Arabidopsis wild type (Col-O ecotype), transgenic Arabidopsis plants (ABI5OE) and mutant abi5-1 plants.

[0099] Plant seeds were sown on MS medium containing different concentrations of 3-AT (0 mM, 5 mM) (80-100 seeds per plant). After 3 days of low-temperature stratification at 4°C, they were transferred to a light incubator. Record the germination number of seeds at 24h, 48h and 60h after stratification, and make statistics on the results. In the experiment, the plants transformed with the pCAMBIA-1300-221 empty vector obtained in Example 1 were set as a control. The t-test was used to analyze the significance of the difference (**P<0.01). The experiment was repeated 3 times, and the results were averaged.

[0100] The result is as image 3 shown. The results showed that on the medium containing 0mM 3-AT, the seed germi...

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Abstract

The invention discloses application of ABI5 protein and its coding gene in regulating oxidative stress resistance of plant seeds. The application of the invention is any one of the following applications (a1)-(a6) of protein composed of amino acid sequences as shown in Sequence 3 in the sequence table: (a1) regulating oxidative stress resistance of plants; (a2) raising oxidative stress resistanceof plants; (a3) breeding plant varieties with enhanced oxidative stress resistance; (a4) regulating resistance of plants to herbicides; (a5) raising resistance of plants to herbicides; and (a6) breeding plant varieties with enhanced resistance to herbicides. The application of the invention is helpful for the research on molecular mechanism of oxidative stress resistance in the plant seed germination process, improvement of seed quality and stress-resistance traits and breeding of plants with oxidative stress resistance, meets requirements of agricultural production, brings greater economic benefits to crop production, and has important practical value and market prospect.

Description

technical field [0001] The invention relates to the application of an ABI5 protein and its coding gene in regulating the resistance to oxidative stress of plant seeds. Background technique [0002] The life of a plant begins with seeds, and seed germination is restricted by external environmental conditions, such as drought, low temperature and high salinity, and oxidative stress. When the plant is subjected to environmental stress conditions, it will immediately induce various related oxidative stress reactions in the plant, and the generation of active oxygen in the body will far exceed the scavenging ability of the antioxidant system, causing severe oxidative stress in the cell, and the stable state of intracellular active oxygen Severely damaged, a large amount of active oxygen is produced, the activity of antioxidant enzymes is reduced, cell damage is caused, and the metabolism of damaged cells is affected from different aspects. These destructive effects include: ① ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10
CPCC07K14/415C12N15/8271C12N15/8274
Inventor 王小芳毕超马宇张大鹏
Owner TSINGHUA UNIV
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