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C-reactive protein magnetic particle detection kit and use method thereof

A technology for detecting kits and reactive proteins, which is applied in biological testing, analysis through chemical reactions of materials, and material inspection products. It can solve the problems of storage stability impact and poor detection flexibility, and avoid stable storage The effect of improving sensitivity and improving flexibility

Pending Publication Date: 2018-02-02
SUZHOU SYM BIO LIFESCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reagents used in this method are all in large packages, and are added to the reaction tube by a pipette for reaction during detection. This method has the advantages of high sensitivity, good specificity, and wide detection range, but due to the limitation of the detection method of its packaging box, The flexibility of its detection is poor, and once the reagent is opened, its storage stability will be affected by the surrounding environment

Method used

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  • C-reactive protein magnetic particle detection kit and use method thereof
  • C-reactive protein magnetic particle detection kit and use method thereof
  • C-reactive protein magnetic particle detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] like figure 1 As shown, this embodiment provides a C-reactive protein detection kit, including a reagent strip composed of several wells arranged side by side, and the reagents contained in the reagent strip include CRP magnetic bead working solution, CRP acridinium ester labeling working solution liquid. The kit also contains sample diluent, calibrators, bead wash solution, and pre-challenge solution. The corresponding reagents are sealed and stored in the corresponding holes on the reagent strips using heat-sealing film technology, which effectively prevents the reagents from crossing holes and solves the transportation problem.

[0087] On the C-reactive protein detection kit of this embodiment, a magnetic bar cover for use with a special up-suction magnetic bead transfer gun is provided in a hole on the reagent strip, so that when the kit is used for C-reactive protein detection , using the up-suction magnetic bead transfer gun to transfer the magnetic beads, the ...

Embodiment 2

[0090] This embodiment provides a method for preparing a C-reactive protein detection kit (magnetic particle chemiluminescence method), which specifically includes the following steps:

[0091] One, the selection of instrument, raw material, reagent adopted in the present embodiment:

[0092] (1) Instruments and sources used in this example:

[0093] equipment name

Manufacturer

TDL-60B low speed desktop centrifuge

Haier refrigerator

Haier

Haier refrigerator

Haier

Six-station magnetic stirrer

Six-station magnetic stirrer

MX-S adjustable mixer

MX-S adjustable mixer

THZ-D desktop constant temperature oscillator

Computer UV detector

TDL-60B low speed desktop centrifuge

SW-TFG-15 fume hood

HD-5 Computer UV Detector

HL-2 constant flow pump

HPS-250 biochemical incubator

Reagent strip packaging equipment

Guang...

Embodiment 3

[0147] This embodiment provides a method for using the kit to detect C-reactive protein. During detection, the reagent strip 4 is placed in the incubation chamber 3 and the incubation chamber 3 is set on the conveyor belt 2, and the conveyor belt 2 is connected to the horizontal displacement The end of the motor 1 and the conveyor belt 2 is provided with an analysis reading module 13, and an up-suction magnetic bead transfer gun that can move up and down relative to the magnetic rod cover 5 is used to realize the extraction of the magnetic rod cover, magnetic beads, and magnetic beads from the reagent strip. Elution, magnetic bead mixing, and removal of the magnetic bar cover; specifically include the following steps:

[0148] Step 1, select the project: select the CRP project according to the sample, insert the reagent strip into the reaction channel of the instrument, and click start;

[0149] Step 2, scan the code: the instrument scans the QR code on the surface of the seal...

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Abstract

The invention discloses a C-reactive protein detection kit and a use method thereof. The kit comprises a reagent strip composed of a plurality of hole sites arranged side by side, and reagents in thereagent strip contain a CRP magnetic bead working solution and a CRP acridinium ester-labeled working solution; and the kit further comprises a calibrating substance, a pre-excitation solution, an excitation solution and a magnetic bead cleaning solution, and a magnetic rod sleeve is arranged in one of the hole sites on the reagent strip. The C-reactive protein detection kit and the use method thereof are based on a chemiluminescence immunoassay technique, and has the advantages of improvement of the sensitivity, the specificity and the precision of C-reactive protein detection, realization ofadaptation of detection to many sample types, and maximal elimination of the influences of sample differences on the detection.

Description

technical field [0001] The invention relates to the technical field of C-reactive protein detection, in particular to a C-reactive protein detection kit and a use method thereof. Background technique [0002] C-reactive protein (C-reactive protein, CRP) is synthesized by hepatocytes, produced during fetal life, and passed through the non-maternal placenta. Its production mechanism is: when the body is infected or the tissue is damaged, macrophages and other white blood cells are activated to produce interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor Cytokines such as TNF-a and other mediators, these cytokines and mediators reach the liver and stimulate hepatocytes and epithelial cells to synthesize CRP. Structurally, CRP contains 5 polypeptide chain subunits, which are non-covalently combined into a disc-shaped polymer with a molecular weight of 115,000 to 140,000. CRP is a typical acute phase protein. [0003] Routine CRP determination includes qualitative,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/76
CPCG01N21/76G01N33/53
Inventor 郝亚澎胡星海
Owner SUZHOU SYM BIO LIFESCI CO LTD
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