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Bacillus coagulans and fermenting method thereof, preparation method for bacillus coagulans preparation and pig feed

A Bacillus coagulans, fermentation method technology, applied in animal feed, animal feed, microbial-based methods, etc., can solve the problems of performance impact, high temperature resistance, limited acid and bile salt resistance, weakening the effect of Bacillus coagulans, etc., To achieve the effect of improving high temperature resistance and application value

Active Publication Date: 2018-02-13
WUHAN POLYTECHNIC UNIVERSITY
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  • Claims
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Problems solved by technology

[0003] However, a large number of Bacillus coagulans currently on the market have limited resistance to high temperature, acid and bile salts, so that Bacillus coagulans can be used in the feed production process (feed production process is often accompanied by high t

Method used

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  • Bacillus coagulans and fermenting method thereof, preparation method for bacillus coagulans preparation and pig feed
  • Bacillus coagulans and fermenting method thereof, preparation method for bacillus coagulans preparation and pig feed
  • Bacillus coagulans and fermenting method thereof, preparation method for bacillus coagulans preparation and pig feed

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[0056] The present invention also provides a preparation method of Bacillus coagulans preparation, image 3 It is an example of the preparation method of the Bacillus coagulans preparation. See image 3 The preparation method of the Bacillus coagulans preparation includes the following steps:

[0057] Step S50, filtering the Bacillus coagulans fermentation liquid to form a concentrated liquid;

[0058] The Bacillus coagulans fermentation broth prepared by the fermentation method of Bacillus coagulans provided in the above steps S10 to S40 is taken out from the fermentor, filtered and concentrated until the supernatant does not flow out substantially, forming the concentrated solution. In the embodiment of the present invention, the specific implementation of the filtration can be performed by using an organic ceramic membrane filtration device, and the Bacillus coagulans fermentation broth is poured into the hopper of the organic ceramic membrane filtration device, and filtered unt...

Example Embodiment

[0064] Example 1 Strain culture

[0065] (1) Strain activation: Place Bacillus coagulans WHQG1 in a liquid medium and culture it at 37°C for 24 hours to obtain activated strains. The preparation method of the medium is as follows: tryptone 10g / L, beef The leaching powder is 3g / L and sodium chloride 5g / L, the pH is adjusted to 6.4-6.6, sterilized at 121°C for 20 minutes and then cooled to room temperature.

[0066] (2) Strain purification: The activated strain in step (1) was inoculated into a solid plate medium and cultured at 37°C for 24 hours to obtain a purified strain. The medium preparation method is as follows: tryptone 10g / L, Beef extract powder 3g / L, sodium chloride 5g / L, and agar 15g / L, adjust the pH to 6.4-6.6, sterilize at 121°C for 20 minutes and then cool to room temperature.

[0067] (3) Preparation of seed solution: select a single colony from the purified strain in step (2), and inoculate it into a liquid medium (same step (1)), the shaker speed is 140r / min, and the ...

Example Embodiment

[0068] Example 2 Fermentation culture

[0069] (1) Fermentation medium pretreatment: Mix the fermentation medium and water at a mass ratio of 1:3, boil for 30 minutes, and filter to obtain a concentrated fermentation medium. The preparation method of the fermentation medium is as follows: corn flour 5g / L , Wheat bran 10g / L, ammonium chloride 7.5g / L, soybean meal powder 7.5g / L, sodium chloride 5g / L, MnSO 4 ·H 2 O 0.3g / L and K 2 HPO 4 ·3H 2 O 1g / L; the pH of the fermentation medium is 6.4-6.6, sterilized at 121°C for 20 minutes and then cooled to room temperature.

[0070] (2) Shake flask fermentation culture: Divide 35 mL of the concentrated fermentation medium prepared in step (1) with 250 mL Erlenmeyer flasks, inoculate 1% of the Bacillus coagulans seed solution prepared in Example 1, and set the shaker speed At 140r / min, shaker fermentation at 40°C for 24h to obtain fermented seed liquid.

[0071] (3) Fermentation culture in a fermentor: Fill a 10L fermentor with 6L of the concent...

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Abstract

The invention discloses bacillus coagulans and a fermenting method thereof, a preparation method for a bacillus coagulans preparation and a pig feed. The bacillus coagulans is the bacillus coagulans WHQG1, and the preservation number thereof is CCTCC NO:M 2015321. According to the preparation method, the preserved bacillus coagulans WHQG1 is fermented and cultured, and the bacillus coagulans preparation is prepared after a fermentation product is adsorbed by using an adsorbent, so the high-temperature resistance, acid resistance and cholate resistance of the bacillus coagulans are improved, and the application value of the bacillus coagulans in the piglet feeding is improved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a bacillus coagulans and a fermentation method thereof, a preparation method of a bacillus coagulans preparation and pig feed. Background technique [0002] Probiotics, as a kind of microorganisms that have no health hazards to humans and animals, have been widely studied at home and abroad. Among them, the research on Bacillus and Lactobacillus is more extensive in my country, and Bacillus coagulans has been widely used because of its excellent biological characteristics. in animal husbandry. Feeding livestock and poultry (taking piglets as an example) with feed containing Bacillus coagulans has the following advantages: First, the metabolism of Bacillus coagulans in the intestinal tract consumes a large amount of oxygen and produces a large amount of lactic acid, which promotes the proliferation of probiotics and inhibits harmful bacteria The reproduction of Baci...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/30C12R1/07
CPCA23K10/18A23K50/30C12N1/20C12R2001/07C12N1/205Y02P60/87
Inventor 吴涛
Owner WUHAN POLYTECHNIC UNIVERSITY
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