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A method for extraction and separation of tetracycline antibiotics in milk powder using metal-organic framework-molecularly imprinted composite materials

A metal-organic framework and molecular imprinting technology, applied in applications, milking devices, and other dairy products, can solve problems such as poor selectivity, complex process, and large consumption of organic solvents, and achieve low utilization rate and high selectivity , Solve the effect of impurity interference

Active Publication Date: 2021-03-26
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of complex process, poor selectivity and large consumption of organic solvents in the existing method for detecting tetracycline antibiotics in milk powder, and to provide a method for extracting and separating tetracycline in milk powder using metal organic framework-molecular imprinting composite materials antibiotic-like approach

Method used

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  • A method for extraction and separation of tetracycline antibiotics in milk powder using metal-organic framework-molecularly imprinted composite materials
  • A method for extraction and separation of tetracycline antibiotics in milk powder using metal-organic framework-molecularly imprinted composite materials
  • A method for extraction and separation of tetracycline antibiotics in milk powder using metal-organic framework-molecularly imprinted composite materials

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specific Embodiment approach 1

[0020] Specific implementation mode 1: This implementation mode is a method for extracting and separating tetracycline antibiotics in milk powder by using a metal organic framework-molecularly imprinted composite material, which is completed according to the following steps:

[0021] Firstly, the milk powder is dried to obtain dry milk powder, and then the dried milk powder, metal-organic framework-molecularly imprinted composite material and Na 2 Put EDTA into an agate mortar and grind for 5min to 15min to obtain the ground mixture, put the ground mixture into the extraction column for compaction, place a sieve plate at the upper and lower ends of the extraction column, and then use the volume fraction as 10%~ 30% acetonitrile aqueous solution is used as the eluent for elution, the elution rate is 1.0mL / min~2.0mL / min, and then acetic acid methanol solution is used as the eluent for elution, the elution rate is 1.0mL / min~2.0 mL / min, the acetic acid methanol solution is formed ...

specific Embodiment approach 2

[0026] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the preparation process of the metal-organic framework-molecularly imprinted composite material is as follows:

[0027] One, ①, ultrasonically dissolve terephthalic acid in N, N dimethyl formamide solution, obtain the solution of terephthalic acid, the quality of terephthalic acid described in step ① and N, N dimethyl formamide The volume ratio of the formamide solution is 1g:(50-60)mL; ②, ultrasonically dissolve aluminum trichloride in N, N dimethylformamide solution to obtain a solution of aluminum ions, step 1 and ② described in the third The volume ratio of the mass of aluminum chloride to the N,N dimethylformamide solution is 1g:(75-80)mL; ③, add the solution of terephthalic acid to the solution of aluminum ions drop by drop, and mix well, Obtain a colorless oily solution, transfer the colorless oily solution to a polytetrafluoroethylene reaction kettle, and then place it in a blast dryin...

specific Embodiment approach 3

[0034] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is: the aperture of the sieve plate placed at the upper end of the extraction column is 20 μm, and the aperture of the sieve plate placed at the lower end of the extraction column is 20 μm. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for extracting and separating tetracycline antibiotics in milk powder by utilizing a metal organic framework-molecular imprinting compound material, and relates to a method for extracting and separating antibiotic residues in the milk powder. The invention aims to solve the problems that existing methods for detecting tetracycline antibiotics in milk powder have complex processes, poor selectivity and large organic solvent consumption. The method of the invention comprises taking the metal organic framework-molecular imprinting compound material as an adsorbent, taking Na2EDTA as a masking agent, mixing the adsorbent, the masking agent and milk powder to prepare an extraction column, taking an acetonitrile aqueous solution as a leaching liquid, conducting leaching, utilizing an acetic acid methanol solution as an eluant, and conducting elution so as to finish the extraction separation of tetracycline antibiotics in the milk powder. The method has the advantages that the adsorption amount of molecular imprinting is improved. The selection coefficient of a detection target analyte is 2.5 to 3.5. The method is mainly used for extracting and separatingtetracycline antibiotics in milk powder.

Description

technical field [0001] The invention relates to a method for extracting and separating antibiotic residues in milk powder. Background technique [0002] Tetracycline antibiotics are broad-spectrum antibiotics, mainly including chlortetracycline, oxytetracycline and tetracycline. Because of their bactericidal effect at high concentrations, they are widely used in livestock and poultry farming and medicine. However, due to the increasing use of tetracycline antibiotics and their long-term retention in food (such as milk powder), coupled with their wide application, the problem of drug resistance in humans and animals caused by them is becoming more and more prominent. It is of great significance to detect the content of tetracycline antibiotics in food. [0003] Molecular imprinting originated from immunology. Because the recognition of molecularly imprinted polymers and template molecules is highly selective, just like the recognition of antibodies and antigens, molecular im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C7/04
CPCA23C7/043
Inventor 牛娜王尚书陈立钢
Owner NORTHEAST FORESTRY UNIVERSITY
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