Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of genistein in preparation of heavy ion radiation-sensitizing drug

A technology of genistein and heavy ion radiation, applied in the field of biomedicine, to achieve the effect of reducing the number of treatments, reducing the cost of treatment, and improving the killing efficiency

Inactive Publication Date: 2018-02-16
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with X-rays, heavy ions mainly induce DNA double-strand breaks and DNA cluster damage. The main reason why tumor cells have strong radiation resistance is that tumor cells have a relatively complete DNA repair system. If It can effectively inhibit the repair of DNA double-strand breaks, kill tumor cells to a greater extent, and achieve twice the result with half the effort. Therefore, it is urgent to solve the problem of improving the therapeutic effect of heavy ions on tumor cells with strong radiation resistance and improving the utilization of heavy ion beams. rate problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of genistein in preparation of heavy ion radiation-sensitizing drug
  • Application of genistein in preparation of heavy ion radiation-sensitizing drug
  • Application of genistein in preparation of heavy ion radiation-sensitizing drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. Cells and Reagents

[0033] M059K and U87 are radiation-resistant human glioma cells, originally purchased from the ATCC cell bank in the United States, and then cultured and stored in our laboratory; DMEM / F12 and DMEM medium were purchased from Gibco; fetal bovine serum (FBS) was purchased from Corning; Genistein and Nu7026 were purchased from Sigma; and biochemical reagents such as dimethyl sulfoxide (DMSO) and Triton X-100 were purchased from Gansu Pengcheng Biotechnology Development Co., Ltd. Using the high energy of the Institute of Modern Physics, Chinese Academy of Sciences 12 C 6+ The heavy ion beam is used as the radiation source to irradiate the experimental materials.

[0034] experimental method

[0035] 2.1 Cell culture and drug treatment

[0036] Human glioma M059K and U87 cells were cultured in a complete medium containing 10% FBS in DMEM / F12 and DMEM, respectively, in a 5% carbon dioxide, 37°C saturated humidity incubator, and the logarithmic growth phase ce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of genistein in the preparation of a heavy ion radiation-sensitizing drug. Effective concentration of the genistein as a radiation-sensitizing drug in the preparation of a heavy ion radiation-sensitizing drug for inhibiting tumor proliferation of patients is 5-20 microns. The drug can remarkably inhibit heavy ion radiation induced DNA double-strand break repairand promote tumor cell apoptosis so as to further raise tumor cell killing efficiency of heavy ions, has potential application value in the preparation of a radiosensitivity drug, and is helpful forenhancing the effect of single irradiation in heavy ion treatment, reducing the number of treatment, shortening treatment course and lowering treatment cost.

Description

Technical field [0001] The present invention relates to the technical field of biomedicine, in particular to the application of genistein in the preparation of heavy ion radiation sensitizing drugs. Background technique [0002] Cancer is one of the leading causes of death in the world’s population. The World Health Organization’s 2014 Cancer Report pointed out that there were approximately 14 million new cancer cases and 8.2 million cancer-related deaths in 2012. In the next 20 years, it is estimated that the annual cancer cases will rise from 14 million in 2012 to 22 million. In addition, China ranks first in the world in terms of new and death cases. There were 4.29 million new cancer cases and 2.81 million cancer deaths in China in 2015 [Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ, He J. Cancer statistics in China. CA Cancer JClin. 2016, 66,115-32.]. [0003] Radiation has been used in tumor treatment for many years. Radiation therapy is used for 70% t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352
Inventor 刘雄雄李强陈卫强李萍金晓东张鹏程赵婷郑小刚戴中颖
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com