Probiotic and high-efficiency toxin adsorbent and its production method and application
A technology of toxin adsorbent and probiotics, applied in the field of feed, can solve the problems of unsatisfactory adsorption effect of broad-spectrum mycotoxins, poor solubility, and small contact area of mycotoxins.
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[0051] Preparation of Yeast Cell Wall Extract
[0052] The yeast cells are then lysed. Lysis can be autolysis or hydrolysis, and any of the methods mentioned in the journal literature can be used to lyse yeast cells. In the present invention, autolysis of yeast cells is performed at a temperature of about 50°C. After lysis, the resulting cell walls were centrifuged several times to remove intracellular material and concentrate the cell walls.
[0053] Cook the obtained yeast cell wall in a cooking pot at a temperature of 80-98°C for 1-2 hours, then lower the temperature to 40-60°C, adjust the pH value to 7-8, and then add an appropriate proportion of biological enzymes, the enzymes used in the process Selected from alkaline protease, mannosidase and amylase or their mixtures, preferably alkaline protease. In a specific embodiment, 1-4wt‰ of alkaline protease and 1-4wt‰ of mannosidase are used for enzymatic hydrolysis at 40-60° C. for 15-16 hours. After centrifugation, th...
Embodiment 1
[0061] Embodiment 1 Mannan oligosaccharide preparation
[0062] 300 g of Saccharomyces cerevisiae (Dalian Xinghe Yeast Co., Ltd., China) were autolyzed at a temperature of about 50 ° C, and the resulting cell walls were centrifuged in a centrifuge at a speed of 6000 rpm to remove intracellular matter and concentrate the cell walls. Cook the obtained yeast cell wall in a cooking pot at a temperature of 80-98° C. for 1.5 hours, then lower the temperature to 40-60° C., and adjust the pH value to 7-8. Based on the quality of Saccharomyces cerevisiae, add 1-4‰ weight of alkaline protease and 1-4‰ weight of mannosidase and perform enzymatic hydrolysis at 43° C. for 15-16 hours. After centrifugation, the clear liquid is concentrated, and the obtained clear liquid is spray-dried to obtain hygroscopic water-soluble powder mannan oligosaccharides. After being determined by high performance liquid chromatography (CM5310 12A10-036, Hitachi, Japan), spray drying yielded dry powder 1# with...
Embodiment 2
[0063] Embodiment 2 Mannan oligosaccharide preparation
[0064] Autolyze 300 g of Saccharomyces cerevisiae (obtained by enlarged culture of the Saccharomyces cerevisiae strain M2016418 cultivated by the applicant company) at a temperature of about 50°C, and centrifuge the obtained cell walls in a centrifuge at a speed of 6000 rpm to remove intracellular matter and condense the cell wall. Cook the obtained yeast cell wall in a cooking pot at a temperature of 80-98° C. for 2 hours, then lower the temperature to 40-60° C., and adjust the pH value to 7-8. Based on the quality of Saccharomyces cerevisiae, 1-4‰ weight of alkaline protease and 1-4‰ weight of mannosidase were added for enzymolysis at 60° C. for 15-16 hours. After centrifugation, the clear liquid is concentrated, and the obtained clear liquid is spray-dried to obtain hygroscopic water-soluble powder mannan oligosaccharides. The dry powder 2# with mannan oligosaccharide content reaching 48wt% was obtained by spray dry...
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