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A two-phase deep eutectic solvent for extracting ginkgo biloba active components and its preparation method and extraction method

A technology of co-melting solvents and active ingredients, applied in the field of utilization of agricultural and forestry biological resources, can solve the problems of increasing extraction steps, increasing extraction costs, and being volatile

Active Publication Date: 2021-05-18
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, water or organic solvents are usually used as extraction solvents for the extraction of active components of ginkgo biloba. Water as an extraction solvent has poor extraction effect, while traditional organic solvents are volatile and easy to remain, causing harm to the environment and human body, and high cost
In addition, since ginkgo flavonoids, terpene lactones, and proanthocyanidins are hydrophilic, and polyprenyl acetate is hydrophobic, the previous methods for extracting the active ingredients of ginkgo biloba were to extract these ingredients separately or to extract the hydrophilic ingredients. The active and hydrophobic components are extracted separately, which on the one hand causes waste of bioactive components, and on the other hand increases the extraction steps and increases the extraction cost

Method used

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  • A two-phase deep eutectic solvent for extracting ginkgo biloba active components and its preparation method and extraction method
  • A two-phase deep eutectic solvent for extracting ginkgo biloba active components and its preparation method and extraction method
  • A two-phase deep eutectic solvent for extracting ginkgo biloba active components and its preparation method and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Weigh choline chloride and levulinic acid in a molar ratio of 1:2 and mix them evenly, heat and stir at 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES1, mix DES1 and water Mix according to mass ratio 6:4 to obtain substance A;

[0049] (2) Weigh choline chloride and malonic acid in a molar ratio of 1:2 and mix them evenly, heat and stir between 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES2, and DES2 and water Mix according to mass ratio 4.5:5.5 to obtain substance B;

[0050] (3) Material A and material B are mixed by volume ratio 8.75:1.25 to obtain the hydrophilic phase of two-phase deep eutectic solvent;

[0051] (4) Weigh methyl trioctyl ammonium chloride, octanol and octanoic acid in a molar ratio of 1:2:3 and mix them evenly, heat between 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES3 , as the hydrophobic phase of the two-phase deep eutecti...

Embodiment 2

[0054] (1) Weigh choline chloride and levulinic acid in a molar ratio of 1:2.5 and mix them evenly, heat and stir between 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES1, and DES1 and water Mix according to mass ratio 6:4.5 to obtain substance A;

[0055] (2) Weigh choline chloride and malonic acid in a molar ratio of 1:2.5 and mix them evenly, heat and stir between 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES2, and DES2 and water Mix according to mass ratio 4.5:6 to obtain substance B;

[0056] (3) material A and material B are mixed by volume ratio 7.75:2.25 to obtain the hydrophilic phase of two-phase deep eutectic solvent;

[0057] (4) Weigh methyl trioctyl ammonium chloride, octanol and caprylic acid in a molar ratio of 1:2:3.5 and mix them evenly, heat between 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES3 , as the hydrophobic phase of the two-phase d...

Embodiment 3

[0060] (1) Weigh choline chloride and levulinic acid in a molar ratio of 1:1.5 and mix them evenly, heat and stir at 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES1, and DES1 and water Mix according to mass ratio 6:3.5 to obtain substance A;

[0061] (2) Weigh choline chloride and malonic acid at a molar ratio of 1:1.5 and mix them evenly, heat and stir between 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES2, and DES2 and water Mix according to mass ratio 4.5:5 to obtain substance B;

[0062] (3) Material A and material B are mixed by volume ratio 8.75:1.25 to obtain the hydrophilic phase of two-phase deep eutectic solvent;

[0063] (4) Weigh methyl trioctyl ammonium chloride, octanol and caprylic acid in a molar ratio of 1:2:2.5 and mix them evenly, heat at 80-110°C until a transparent liquid is formed, which is the deep eutectic solvent DES3 , as the hydrophobic phase of the two-phase deep eutect...

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Abstract

The invention discloses a two-phase deep eutectic solvent for extracting active components of ginkgo biloba leaves and its preparation method and extraction method. The two-phase deep eutectic solvent is composed of a hydrophilic phase and a hydrophobic phase of equal volume, and the hydrophilic phase consists of Composition of substance A and substance B, substance A is composed of DES1 and water, DES1 is prepared from choline chloride and levulinic acid; substance B is composed of DES2 and water, DES2 is prepared from choline chloride and malonic acid The hydrophobic phase was prepared from methyltrioctylammonium chloride, octanol, and octanoic acid. The two-phase deep eutectic solvent prepared by the invention is a green solvent, which is safe and pollution-free, has low viscosity, stable hydrogen bonds, and good fluidity; it can simultaneously extract the hydrophobic active ingredient polyprenyl acetate and hydrophilic The active ingredients ginkgo flavonoids, terpene lactones and proanthocyanidins have a high extraction rate, are safe to use, and will not form residues. The preparation method of the deep eutectic solvent of the present invention is simple and convenient, low in cost and high in biodegradability.

Description

technical field [0001] The invention relates to the utilization of agricultural and forestry biological resources, in particular to a two-phase deep eutectic solvent for extracting ginkgo biloba active components, a preparation method and an extraction method thereof. Background technique [0002] Ginkgo biloba (scientific name: Ginkgo biloba L.), also known as Gongsun tree and ginkgo, belongs to gymnosperm, Ginkgo family Ginkgo genus, single genus and single species. Ginkgo biloba, fruit, etc. have medicinal development value, and are known as living fossils full of treasures. Ginkgo is native to my country, and my country's ginkgo resources account for more than 70% of the world's total. [0003] The active ingredients of Ginkgo biloba mainly include ginkgo flavonoids, terpene lactones, proanthocyanidins, polyprenyl alcohol, etc. The content of flavonoids in Ginkgo biloba ranks first, accounting for about 2.5-5.9% of the total content. More than 40 kinds of flavonoids ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02A61K36/16A61K127/00
CPCA61K36/16A61K2236/333A61K2236/53
Inventor 苏二正曹君李默涵曹福亮陈璐瑶赵林果王耀松
Owner NANJING FORESTRY UNIV
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