Anthocyanin extracted and purified from perilla leaves and preparation method of anthocyanin
An anthocyanin and perilla leaf technology is applied in the field of extracting and purifying anthocyanin and its preparation, which can solve the problems of difficulty in industrialized production, high food safety risk and high production cost, and achieves less environmental pollution and selective adsorption capacity. Strong, high recovery effect
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specific Embodiment approach 1
[0031] An anthocyanin extracted and purified from perilla leaves, the main component of the anthocyanins extracted and purified from perilla leaves is cyanidin-3-O-glucoside, and the cyanidin-3-O-glucoside is The structural formula of 3-O-glucoside is C 21 h 21 ClO 11 .
specific Embodiment approach 2
[0032] The preparation method for extracting and purifying anthocyanins from perilla leaves according to specific embodiment 1 comprises the following steps:
[0033] Step 1, treatment of perilla leaves: Harvest perilla leaves at the mature stage of perilla, and dry the perilla leaves under natural conditions or dry them at low temperature and crush them for later use;
[0034] Step 2, the configuration of the extract: choose acetic acid with a concentration of 7% as the extraction reagent, choose absolute ethanol as the extraction reagent, and mix the acetic acid water and the ethanol evenly according to the volume ratio of 1:1 to obtain extract, for use;
[0035] Step 3. Extraction of anthocyanins: Weigh 1g of the perilla leaf sample treated in step 1, put it into a test tube, then add 30mL of the extract prepared in step 2 to the test tube, perform ultrasonic extraction, and control the extraction temperature to 35°C. The ultrasonic time is 30min, the ultrasonic frequency is...
specific Embodiment approach 3
[0051] The preparation method for extracting and purifying anthocyanins from perilla leaves according to specific embodiment 1 comprises the following steps:
[0052] Step 1, treatment of perilla leaves: Harvest perilla leaves at the mature stage of perilla, and dry the perilla leaves under natural conditions or dry them at low temperature and crush them for later use;
[0053] Step 2, the configuration of the extract: choose acetic acid with a concentration of 11% as the extraction reagent, choose absolute ethanol as the extraction reagent, mix the acetic acid water and the ethanol evenly according to the volume ratio of 1:1, and obtain extract, for use;
[0054] Step 3. Extraction of anthocyanins: Weigh 1g of the perilla leaf sample treated in step 1, put it into a test tube, then add 30mL of the extract prepared in step 2 to the test tube, perform ultrasonic extraction, and control the extraction temperature to 30°C. The ultrasonic time is 40min, the ultrasonic frequency i...
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