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Anthocyanin extracted and purified from perilla leaves and preparation method of anthocyanin

An anthocyanin and perilla leaf technology is applied in the field of extracting and purifying anthocyanin and its preparation, which can solve the problems of difficulty in industrialized production, high food safety risk and high production cost, and achieves less environmental pollution and selective adsorption capacity. Strong, high recovery effect

Inactive Publication Date: 2018-03-13
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, my country has carried out in-depth research on the extraction process of perilla anthocyanins. For example, a Chinese patent with publication number CN104069172A discloses a perilla leaf extract and its extraction method, and a Chinese patent with publication number CN105801391A discloses the The method for extracting perilla aldehyde and anthocyanin from perilla, the Chinese patent with publication number CN102964866A discloses the method for extracting perilla pigment from perilla leaves, the Chinese patent with publication number CN105614446A discloses the preparation method of perilla extract, but The process routes reported in the above literatures have cumbersome methods, complex processes, use of toxic chemical reagents, high production costs, and high food safety risks, which are difficult to meet the needs of industrial production

Method used

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  • Anthocyanin extracted and purified from perilla leaves and preparation method of anthocyanin
  • Anthocyanin extracted and purified from perilla leaves and preparation method of anthocyanin
  • Anthocyanin extracted and purified from perilla leaves and preparation method of anthocyanin

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specific Embodiment approach 1

[0031] An anthocyanin extracted and purified from perilla leaves, the main component of the anthocyanins extracted and purified from perilla leaves is cyanidin-3-O-glucoside, and the cyanidin-3-O-glucoside is The structural formula of 3-O-glucoside is C 21 h 21 ClO 11 .

specific Embodiment approach 2

[0032] The preparation method for extracting and purifying anthocyanins from perilla leaves according to specific embodiment 1 comprises the following steps:

[0033] Step 1, treatment of perilla leaves: Harvest perilla leaves at the mature stage of perilla, and dry the perilla leaves under natural conditions or dry them at low temperature and crush them for later use;

[0034] Step 2, the configuration of the extract: choose acetic acid with a concentration of 7% as the extraction reagent, choose absolute ethanol as the extraction reagent, and mix the acetic acid water and the ethanol evenly according to the volume ratio of 1:1 to obtain extract, for use;

[0035] Step 3. Extraction of anthocyanins: Weigh 1g of the perilla leaf sample treated in step 1, put it into a test tube, then add 30mL of the extract prepared in step 2 to the test tube, perform ultrasonic extraction, and control the extraction temperature to 35°C. The ultrasonic time is 30min, the ultrasonic frequency is...

specific Embodiment approach 3

[0051] The preparation method for extracting and purifying anthocyanins from perilla leaves according to specific embodiment 1 comprises the following steps:

[0052] Step 1, treatment of perilla leaves: Harvest perilla leaves at the mature stage of perilla, and dry the perilla leaves under natural conditions or dry them at low temperature and crush them for later use;

[0053] Step 2, the configuration of the extract: choose acetic acid with a concentration of 11% as the extraction reagent, choose absolute ethanol as the extraction reagent, mix the acetic acid water and the ethanol evenly according to the volume ratio of 1:1, and obtain extract, for use;

[0054] Step 3. Extraction of anthocyanins: Weigh 1g of the perilla leaf sample treated in step 1, put it into a test tube, then add 30mL of the extract prepared in step 2 to the test tube, perform ultrasonic extraction, and control the extraction temperature to 30°C. The ultrasonic time is 40min, the ultrasonic frequency i...

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Abstract

The invention discloses anthocyanin extracted and purified from perilla leaves and a preparation method of the anthocyanin, and belongs to the technical field of extraction and purification of plant components. Cornflower-3-O-glucoside is a main component in the anthocyanin extracted and purified from the perilla leaves. The preparation method comprises treatment of the perilla leaves, preparationof an extracting solution, extraction of the anthocyanin, pretreatment of adsorption resin, enhanced treatment of the adsorption resin, purification of the anthocyanin and drying of the anthocyanin.According to the preparation method of the anthocyanin, by use of food-grade reagents such as ethyl alcohol and acetic acid for extraction, macroporous adsorption resin for selective purification andan ethyl alcohol-water reagent for elution, the product recycling rate is high, and the anthocyanin content is high. The macroporous adsorption resin adopted in the preparation method is high in selectivity, and other impurities can be effectively removed; the adopted extraction reagent is safe, environmentally friendly, and applicable to the food industry. The obtained anthocyanin product has a high color value and high stability and can be applied to the field of food and medicines; the process method is simple, low in cost and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of plant components, and specifically relates to anthocyanins extracted and purified from perilla leaves and a preparation method thereof. Background technique [0002] Perilla, an annual herb of the Lamiaceae family, is widely planted in Asian countries and has been used in food and medicine for hundreds of years. Perilla is one of the first batch of homologous varieties of medicine and food approved and promulgated by the Ministry of Health, and has become a characteristic plant resource in my country. Usually, perilla can be divided into two chemical types: perilla and green perilla according to the color of the diameter and leaves. Perilla is mainly produced due to the chemical type of anti-oxidation. It has a large anthocyanin content and can be used as a natural food coloring. Additives are widely distributed in perilla planting in my country. Green Su is also rich in ch...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 高宝昌魏国江张旭袁志刚徐海军田媛石雨程薪宇崔宝玉
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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