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A kind of preparation method of traditional Chinese medicine extract for growth and development of allelopathic toona sinensis seeds

A growth and extraction technology, applied in the agricultural field, can solve the problems of less research on seed inhibition, weak root and stem inhibition effects, etc.

Active Publication Date: 2020-06-26
阜阳津科农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

in the treatment of inflammation [15 and cancer [16] It has a very good effect on the aspect, but there are few studies on the allelopathic effect of other plants. Luo Xiaoyong et al. have studied the herbicidal activity of different organs of peony. The research shows that the fruit, leaves and flowers of peony have the strongest inhibitory activity, while the roots and The inhibitory effect of the stem is relatively weak [14]
However, little research has been done on seed inhibition

Method used

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  • A kind of preparation method of traditional Chinese medicine extract for growth and development of allelopathic toona sinensis seeds
  • A kind of preparation method of traditional Chinese medicine extract for growth and development of allelopathic toona sinensis seeds
  • A kind of preparation method of traditional Chinese medicine extract for growth and development of allelopathic toona sinensis seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: The influence of Radix Paeoniae Alba extract on the germination rate of Toona sinensis

[0058] Determination of germination rate

[0059] The radicle length is equal to 1 / 2 of the seed length as the germination standard. When 1 seed germinated in 3 repetitions, this time was recorded as the initial period of germination of the seed, and the number of germinated seeds was recorded every day thereafter. When no seeds germinated for 4 consecutive days, it was recorded as the end of germination. Calculate the germination rate according to the "Seed Biology Research Guide". The indexes of the control at the end of germination were marked as 100%, and the relative values ​​of the indexes after paeoniflorin treatment were calculated. The germination rates of the clear water control group, 5% peony solution, and 10% peony solution were 81.1%, 76.7%, and 60.0%; the standard deviations were 9.62, 3.33, and 11.55; the standard errors were 5.55, 1.92, and 6.67.

...

Embodiment 2

[0062] Embodiment 2: The influence of Radix Paeoniae Alba extract on the root length of Toona sinensis

[0063] Soak 30 Toona sinensis seeds in ultrapure water, 5% peony solution, and 10% peony solution respectively, place them in a constant temperature water bath with a temperature of 30° C., and keep warm for 12 hours. Then place evenly in a Petri dish covered with 1 layer of filter paper pre-soaked with 10ml of ultrapure water (slight residual liquid oozes out). Place temperature then and be to cultivate in the light incubator of 25 ℃, after two weeks, measure the length of the root of each group of seed respectively and calculate mean value, concentration is that 5% and 10% peony aqueous extract all show that toon seed has Strong inhibitory effect (such as figure 2 Shown), clear water control group, 5% peony solution, 10% peony solution root length are respectively 64.4cm, 25.7cm, 15.8cm; standard deviation is 4.49,6.28,3.92; standard error is 2.59,3.62,2.266.

[0064] ...

Embodiment 3

[0065] Example 3: Effects of Radix Paeoniae Alba Extract on the Ratio of Radicle and Epicotyl of Toona sinensis Seeds

[0066] Soak 30 Toona sinensis seeds in ultrapure water, 5% peony solution, and 10% peony solution respectively, place them in a constant temperature water bath with a temperature of 30° C., and keep warm for 12 hours. Then place evenly in a Petri dish covered with 1 layer of filter paper pre-soaked with 10ml of ultrapure water (slight residual liquid oozes out). Then place them in a light incubator with a temperature of 25° C. for cultivation. After two weeks, measure the length of the radicle and epicotyl after the germination of Toona sinensis seeds, and perform corresponding calculations.

[0067] In the early stage of Chinese toon seed growth, the proportion of the control group is always higher than that of the experimental group, indicating that the aqueous extract of peony has a certain delay effect on the radicle and epicotyl ratio of the toon seed, a...

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Abstract

The invention discloses a preparation method of traditional Chinese medicine extract with alleopathic effect on growth and development of cedrela sinensis seeds. The preparation method comprises the following steps: pulverizing Chinese herbaceous peony, soaking with water, boiling, filtering, taking the filtrate as a Chinese herbaceous peony water extract solution to prepare Chinese herbaceous peony water extract solutions with different concentration (5 percent and 10 percent), performing manual treatment on germination of the cedrela sinensis seeds, observing the influence on the growth anddevelopment of the cedrela sinensis seeds by the Chinese herbaceous peony water extract, and detecting the MDA content of the cedrela sinensis root system by a thiobarbituric acid method so as to judge the peroxidation degree of cell membrane lipid and response of the plants on the adverse condition; and quantitatively measuring the vitality of the cedrela sinensis root system by a TTC method so as to discover that the Chinese herbaceous peony water extracts with different concentration have certain delay effects on the germination of the cedrela sinensis seeds, have delay effects on the development of the cedrela sinensis seedlings and can be used for preserving the cedrela sinensis seeds.

Description

technical field [0001] The invention belongs to the field of agriculture, and relates to the sensitizing effect of plant extracts, in particular to a preparation method of a traditional Chinese medicine extract used to sensitize the growth and development of Toona sinensis seeds. Background technique [0002] In recent years, with the development of society, Chinese toon has attracted widespread attention. Chinese toon is not only an edible and nutritious plant, but also its extract contains many medicinal values [1] , Hu Min [2] et al. studied the protective effect of the n-butanol extract of Toona sinensis on liver and lung injury after cerebral ischemia, and found that the n-butanol extract of Toona sinensis can inhibit the damage of multiple organs mediated by cerebral ischemia through its anti-oxidative stress effect. Dysfunctional syndrome plays a protective role. Fuxiong [3] et al. isolated and extracted polyphenols from Toona sinensis leaves, used the activity tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/32A01N65/08A01N3/00A01P21/00
CPCA01N3/00A01N65/00A01N65/08A01N65/32
Inventor 屈长青张秋妍刘晓露杨京霞隋娟娟
Owner 阜阳津科农业科技有限公司