Applications of Isovitexin in preparation of monoamine oxidase (MAO) inhibitors
A technology for monoamine oxidase and inhibitors, which is applied in the application field of preparing monoamine oxidase MAO inhibitors, can solve problems such as limited applications, and achieve the effect of strong inhibitory activity and outstanding substantive characteristics
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Embodiment 1
[0015] Embodiment 1: the preparation of compound Isovitexin tablet involved in the present invention:
[0016] Take 5 grams of compound Isovitexin and add 195 grams of dextrin, mix well, and make 1000 tablets with a conventional tablet machine.
Embodiment 2
[0017] Embodiment 2: the preparation of compound Isovitexin capsules involved in the present invention:
[0018] Take 5 grams of compound Isovitexin, add 195 grams of starch, mix well, and pack into capsules to make 1000 capsules.
[0019] The following pharmacodynamic experiments will further illustrate its drug activity.
[0020] Compound Isovitexin detects the inhibitory activity to MAO-A and MAO-B with the method of fluorescent probe, and described method is carried out according to the following steps:
[0021] Dissolve the sample to be tested in DMSO to prepare a series of samples with concentration gradient. Take 4ul of MAO (10mg / ml), boric acid buffer (PH=8.4), 50ul of BSA (50mg / ml), add the sample solution to be tested, mix well, react in a water bath at 30-38°C for 3h, then add probe 7 -(3-Aminopropoxy)coumarin 2ul (50mmol / ml), the final concentration of the inhibitor is 0~10-2mmol / l, and the mixture is reacted in a water bath at 30±5°C for 12h, and then tested wit...
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