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Method for preparing glycoprotein imprinting fluorescent nanoparticles based on macromolecule self-assembling

A technology of fluorescent nanometer and glycoprotein, which is applied in the direction of fluorescence/phosphorescence, analytical materials, measuring devices, etc., can solve the problems of poor selectivity and achieve good selectivity, fast adsorption speed, and environmental friendliness

Active Publication Date: 2018-04-06
郎溪品旭科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecularly imprinted polymers prepared by these methods are only suitable for organic solvent systems. In aqueous systems, due to the influence of water polarity, the selectivity is poor, and the detection of glycoproteins is usually carried out in aqueous phase, so the development The application of aqueous phase-recognizing molecularly imprinted polymers in glycoprotein sensing has important research significance

Method used

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  • Method for preparing glycoprotein imprinting fluorescent nanoparticles based on macromolecule self-assembling

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Experimental program
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Effect test

Embodiment 1

[0020] The first step: the monomer acrylic acid (0.3603g, 5mmol), vinyl carbazole (0.9663g, 5mmol), vinyl phenylboronic acid (0.0015g, 0.01mmol) azobisisobutylcyanide (0.0328g, 2% mt %) into a 50ml round bottom flask, add solvent N,N-dimethylformamide (DMF) 15mL, pass N 2 After removing the oxygen in the solution for 20min, under stirring, the temperature was kept at 80°C and reacted in an oil bath for 24h. The monomer ethylene glycol methacrylate (0.711g, 5mmol), triphenylphosphine (0.0262g, 0.1mmol) and hydroquinone (0.0028g, 0.025mmol) were dissolved in 5mL of DMF, and were added dropwise to the above polymer solution through a constant pressure dropping funnel. Under stirring, the temperature was maintained at 95°C in oil React in the bath for 12 hours, precipitate with petroleum ether three times, and dry in a vacuum oven at 40°C overnight to obtain a photocrosslinkable amphiphilic random copolymer containing phenylboronic acid;

[0021] Step 2: Dissolve 10 mg of the ab...

Embodiment 2

[0024] The first step: the monomer acrylic acid (0.3603g, 5mmol), vinyl carbazole (0.9663g, 5mmol), vinyl phenylboronic acid (0.0015g, 0.01mmol) azobisisobutylcyanide (0.0328g, 2% mt %) into a 50ml round bottom flask, add solvent N,N-dimethylformamide (DMF) 15mL, pass N 2 After removing the oxygen in the solution for 20min, under stirring, the temperature was kept at 80°C and reacted in an oil bath for 24h. The monomer ethylene glycol methacrylate (0.355g, 2.5mmol), triphenylphosphine (0.0262g , 0.1mmol) and hydroquinone (0.0028g, 0.025mmol) were dissolved in 5mL DMF, and were added dropwise into the above polymer solution through a constant pressure dropping funnel, and the temperature was maintained at 95°C under stirring. React in an oil bath for 12 hours, precipitate with petroleum ether three times, and dry in a vacuum oven at 40°C overnight to obtain a photocrosslinkable amphiphilic random copolymer containing phenylboronic acid;

[0025] Step 2: Dissolve 10 mg of the ...

Embodiment 3

[0028] The first step: the monomer acrylic acid (0.3603g, 5mmol), vinyl carbazole (0.9663g, 5mmol), vinyl phenylboronic acid (0.0015g, 0.01mmol) azobisisobutylcyanide (0.0328g, 2% mt %) into a 50ml round bottom flask, add solvent N,N-dimethylformamide (DMF) 15mL, pass N 2 After removing the oxygen in the solution for 20min, under stirring, the temperature was kept at 80°C and reacted in an oil bath for 24h. The monomer ethylene glycol methacrylate (0.711g, 5mmol), triphenylphosphine (0.0262g, 0.1mmol) and hydroquinone (0.0028g, 0.025mmol) were dissolved in 5mL of DMF, and were added dropwise to the above polymer solution through a constant pressure dropping funnel. Under stirring, the temperature was maintained at 95°C in oil React in the bath for 12 hours, precipitate with petroleum ether three times, and dry in a vacuum oven at 40°C overnight to obtain a photocrosslinkable amphiphilic random copolymer containing phenylboronic acid;

[0029] Step 2: Dissolve 8 mg of the abo...

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Abstract

The invention discloses a method for preparing glycoprotein imprinting fluorescent nanoparticles based on macromolecule self-assembling, relating to the scientific and technical fields of high molecular material science, life health, self-assembling and the like. According to the method, photo-cross-linkable vinyl carbazole containing fluorescent units, hydrophilic monomers and a random amphophilic copolymer containing abornic acid identification unit are firstly prepared and are co-assembled with a photoinitiator and a cross-linking agent under the induction of water so as to form nanoparticles, glycoprotein is packaged in the nanoparticles through the covalent binding with boric acid in an assembling process, the structure of the glycoprotein is stabilized by virtue of a photo-crosslinking technique, the pH value is adjusted, the glycoprotein is eluted, and then the prepared nanoparticles can be used for specifically reconogizing and detecting glycoprotein in a water phase. By integrating the advantages that a nano-material is large in specific surface area, a molecular imprinting material is good in selectivity, the sensitivities of the boric acid-glycoprotein covalent binding and a fluorescence detection technique are high, and the prepared nanoparticles can be applied to high-selectivity and high-sensitivity rapid detection of low-abundance glycoprotein in an actual sampleand particularly have important significance to the trace detection of the glycoprotein in clinical diagnosis and curative effect evaluation.

Description

technical field [0001] The present invention relates to scientific and technological fields such as polymer material science, life and health, and self-assembly, and in particular to a method for preparing glycoprotein imprinted fluorescent nanoparticles based on macromolecular self-assembly Background technique [0002] Glycoproteins are macromolecules formed by the covalent bonding of peptide chains and sugar chains. They are widely present in living organisms and have a variety of biological activities. The well-documented functions include lubrication, transportation, recognition, and protection. Glycoprotein is related to the occurrence and development of many diseases such as infection, tumor, cardiovascular disease, liver disease, kidney disease, diabetes and some genetic diseases. Glycoproteins and sugar esters on the cell surface can "shed" into the surrounding environment or enter the blood circulation, and their detection can provide information for clinical diagn...

Claims

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Application Information

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IPC IPC(8): C08F285/00C08F212/36C08F271/02C08F222/14C08F220/06C08F226/12C08F230/06C08F2/48G01N21/64
CPCC08F2/48C08F220/06C08F226/12C08F265/02C08F271/02C08F285/00G01N21/6428G01N21/6486G01N2021/6432C08F212/36C08F230/06C08F222/102
Inventor 罗静黄婧许升刘晓亚
Owner 郎溪品旭科技发展有限公司
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